Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   K8Z23_RS20400 Genome accession   NZ_CP083129
Coordinates   3959919..3961745 (-) Length   608 a.a.
NCBI ID   WP_000003356.1    Uniprot ID   A0A9X1AZI2
Organism   Bacillus thuringiensis strain NB-176     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IS/Tn 3962262..3963698 3959919..3961745 flank 517


Gene organization within MGE regions


Location: 3959919..3963698
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8Z23_RS20400 (K8Z23_20370) pepF 3959919..3961745 (-) 1827 WP_000003356.1 oligoendopeptidase F Regulator
  K8Z23_RS20405 (K8Z23_20375) - 3961797..3962135 (-) 339 Protein_4061 competence protein -
  K8Z23_RS20410 (K8Z23_20380) - 3962262..3963698 (-) 1437 WP_001053969.1 IS4-like element IS231C family transposase -

Sequence

Protein


Download         Length: 608 a.a.        Molecular weight: 70176.44 Da        Isoelectric Point: 4.5990

>NTDB_id=604741 K8Z23_RS20400 WP_000003356.1 3959919..3961745(-) (pepF) [Bacillus thuringiensis strain NB-176]
MSEQNKAKTLPDRNEIEEASTWRLEDIFQTDAEWEKEFQAIKELLPKLTEFKGKLGDSADNLLEALQYEDEISMRLGKLY
TYAHMRYDQDTTNSVYQALNDRATNLYSQVSSSTAYIVPEILSVSEDTLQTFLKENRDLSVYEHALEEITRQRPHVLSEA
EEALLAEASEVMSSSSNTFGMLNNADLKFPSIKGEDGEEIEITHGRYIQFLESDDGRVREDAFKAVYETYGKFKNTFAST
LSGAVKRNNFNARVRKYDSARQAALSNNNIPEAVYDQLVETVNDNLHLLHRYIDIRKRALGLDELHMYDLYTPLVPEVKM
NVKYEEAQDLLLKSLNVLGDEYVDILKEAYENRWVDVYENKGKRSGAYSSGAYGTNPYILMNWHDNVNNLFTLAHEFGHS
VHSYYTRKTQPHVYGDYSIFVAEVASTCNEALLNDYLLKTTEDKKERLYLLNHYLEGFRGTVFRQTMFAEFEHIIHKKVQ
EGHAVTPDMLTEIYYDLNKKYFGDALVIDEEIGLEWSRIPHFYYNYYVYQYATGFSAATALSKQILEEGQPAVERYINEF
LKAGSSDYPIEVLKKAGVDMASPEPVKEALQVFEEKLNELEALLFEEK

Nucleotide


Download         Length: 1827 bp        

>NTDB_id=604741 K8Z23_RS20400 WP_000003356.1 3959919..3961745(-) (pepF) [Bacillus thuringiensis strain NB-176]
ATGTCTGAACAAAACAAAGCGAAAACATTACCAGATCGCAATGAGATTGAAGAAGCAAGTACGTGGCGATTAGAAGATAT
TTTCCAAACAGATGCAGAATGGGAAAAAGAATTCCAAGCTATTAAAGAGCTATTACCGAAGTTAACTGAATTTAAAGGGA
AACTTGGTGACTCTGCGGACAATTTACTTGAGGCATTGCAATATGAAGACGAAATTTCAATGCGATTAGGTAAGCTATAT
ACATATGCTCATATGCGTTACGATCAAGATACAACAAACTCTGTATATCAAGCATTAAATGATCGCGCAACAAATTTATA
TTCACAAGTATCTAGTAGCACAGCGTATATTGTACCTGAAATTTTGTCGGTTTCAGAAGATACACTGCAAACATTCTTGA
AGGAAAATAGAGATTTAAGTGTATATGAACATGCATTAGAAGAAATTACACGTCAACGCCCGCACGTATTATCAGAAGCT
GAGGAGGCTTTATTAGCGGAAGCATCTGAAGTAATGAGTTCATCAAGCAATACATTTGGTATGTTGAATAACGCGGATTT
AAAATTCCCATCTATTAAAGGTGAAGACGGAGAAGAAATAGAAATTACACATGGTCGTTACATTCAGTTTTTAGAAAGTG
ATGATGGCCGTGTTCGCGAAGATGCATTTAAAGCTGTATATGAAACGTACGGGAAATTTAAAAATACATTCGCAAGTACG
TTAAGCGGAGCAGTAAAACGTAATAATTTCAATGCGCGTGTTCGTAAATATGATTCTGCTCGCCAAGCTGCACTAAGCAA
TAATAATATTCCAGAAGCAGTATATGATCAACTTGTTGAAACGGTAAATGACAACTTACATTTATTACACCGTTACATCG
ATATTCGTAAGCGTGCATTAGGACTTGATGAGCTTCATATGTATGATTTATATACACCACTTGTACCAGAAGTGAAAATG
AATGTGAAATACGAAGAAGCGCAAGACCTTTTATTAAAATCTTTAAACGTACTTGGTGATGAATATGTTGATATTTTGAA
AGAAGCATATGAAAATCGCTGGGTAGATGTGTATGAGAATAAAGGAAAACGAAGCGGGGCATATTCATCTGGTGCATATG
GAACAAATCCGTATATTTTAATGAACTGGCATGATAATGTAAATAATTTATTTACACTTGCTCATGAATTTGGTCATTCG
GTGCATAGTTACTATACAAGAAAAACACAACCGCACGTATATGGTGATTATTCCATCTTCGTAGCAGAAGTGGCATCAAC
TTGTAATGAAGCGCTTCTAAATGATTATTTATTAAAAACGACAGAAGATAAGAAAGAGCGTCTATATTTATTAAATCATT
ATTTAGAAGGATTCCGTGGTACTGTATTCCGTCAAACGATGTTTGCAGAGTTTGAACATATTATTCATAAGAAAGTACAA
GAAGGACATGCTGTTACGCCAGACATGCTAACGGAAATCTACTACGATTTAAATAAGAAATATTTCGGTGACGCTTTAGT
AATCGACGAAGAGATTGGTTTAGAATGGTCTCGTATTCCGCACTTCTACTACAACTATTACGTATATCAATACGCAACAG
GATTTAGTGCAGCGACAGCTCTATCTAAACAGATTTTAGAAGAAGGACAACCAGCAGTAGAACGTTATATTAACGAGTTC
TTAAAAGCAGGAAGCTCTGATTATCCAATTGAAGTGCTGAAAAAAGCAGGAGTAGATATGGCATCTCCTGAACCAGTAAA
AGAGGCACTACAAGTATTTGAAGAGAAATTAAATGAATTAGAAGCATTATTATTTGAAGAAAAGTAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(211-591)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

49.916

97.533

0.487