Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   K8Z39_RS01120 Genome accession   NZ_CP083085
Coordinates   173256..174632 (-) Length   458 a.a.
NCBI ID   WP_001085202.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain B401     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 168256..179632
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8Z39_RS01100 (K8Z39_01095) ispF 169619..170095 (-) 477 WP_000488391.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  K8Z39_RS01105 (K8Z39_01100) ispD 170212..170892 (-) 681 WP_021728590.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  K8Z39_RS01110 (K8Z39_01105) - 170909..172018 (-) 1110 WP_000919677.1 PIN/TRAM domain-containing protein -
  K8Z39_RS01115 (K8Z39_01110) disA 172179..173252 (-) 1074 WP_000392158.1 DNA integrity scanning diadenylate cyclase DisA -
  K8Z39_RS01120 (K8Z39_01115) radA 173256..174632 (-) 1377 WP_001085202.1 DNA repair protein RadA Machinery gene
  K8Z39_RS01125 (K8Z39_01120) clpC 174728..177163 (-) 2436 WP_000971187.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  K8Z39_RS01130 (K8Z39_01125) - 177186..178250 (-) 1065 WP_000050842.1 protein arginine kinase -
  K8Z39_RS01135 (K8Z39_01130) - 178255..178803 (-) 549 WP_000128398.1 UvrB/UvrC motif-containing protein -
  K8Z39_RS01140 (K8Z39_01135) ctsR 178974..179435 (-) 462 WP_001244565.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49844.48 Da        Isoelectric Point: 7.1422

>NTDB_id=604557 K8Z39_RS01120 WP_001085202.1 173256..174632(-) (radA) [Bacillus thuringiensis strain B401]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNSLVEEMEPVVSSRRLNYANAIQTEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADASYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPEGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=604557 K8Z39_RS01120 WP_001085202.1 173256..174632(-) (radA) [Bacillus thuringiensis strain B401]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGTTATCAATCACCAAAATATATGGGTAAATGTCCAGGGTG
TGGTCAATGGAATTCGCTTGTTGAAGAGATGGAACCCGTTGTATCATCCAGACGCCTAAATTATGCAAATGCAATTCAAA
CAGAAGTAACAAAACCAAGACGTCTTACAGAAGTAGAAACAAAGTCTGAGGCACGTATTGAAACAAAATTTCAGGAGTTT
AACCGTGTACTAGGTGGCGGAATTGTAGATGGATCTTTAGTACTTATTGGTGGGGACCCTGGGATTGGAAAATCAACGTT
ATTATTACAGATTTCCTCGCAATTAGCAGATGCTTCATATGATGTATTGTATATATCAGGTGAGGAGTCAGCAAAGCAGA
TTAAACTTCGTGCAGATCGTTTACATGTAAATGGTAGTAATCTATTTGTTGTATCAGAGACAGATTTACAGCGTATTGCA
GCACATATTGAAGAGATGAATCCTGCTTTTGTTGTTATTGATTCTATTCAAACGATACATTTACCTGAGGTGACATCAGC
TCCTGGTAGCGTGGCGCAAGTACGTGAATGTACAGCAGAATTAATGAAACTTGCAAAAACAAAGGGAATCCCAATTTTCA
TCGTAGGACATGTGACAAAAGAAGGTGCAATTGCGGGGCCGCGTATGTTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGATCGACACCATACATATCGTATTTTACGAGCTGTAAAGAATCGTTTTGGTTCTACGAATGAAATGGGTATCTT
TGAAATGAAAGAACTTGGCCTTGCAGAAGTCTTAAATCCTTCTGAAATTTTCCTTGAGGAAAGACCAGTTGGAGTTGCAG
GTTCAACAGTAGTTGCCTCAATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAGGCATTAATTTCCCCTACTAGTTTT
GGAAATCCTCGAAGAATGGCGACAGGAATTGATCATAATCGTGTCTCGCTTATTATGGCGGTACTAGAGAAAAGAACAGG
TTTACTATTACAAAATCAAGATGCGTATTTAAAAGTAGCTGGTGGTTTGAAATTAGATGAACCGGCAATTGATTTAGCTG
TTGCCTTAAGTATAGCTTCAAGTTTTAGAGATAAATCTACGGCACCAACTGATGCGGTAATAGGAGAAGTTGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATCGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGTGCTATCATTCC
TAGAAAAAATTTAGGGGGGTGGACAATTCCAGAGGGGATTGAGGTTGTAGGTGTTTCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

AAA domain (AAA_25)

(60-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.728

100

0.749
  radA Streptococcus pneumoniae Rx1

64.018

98.908

0.633
  radA Streptococcus pneumoniae D39

64.018

98.908

0.633
  radA Streptococcus pneumoniae R6

64.018

98.908

0.633
  radA Streptococcus pneumoniae TIGR4

64.018

98.908

0.633
  radA Streptococcus mitis SK321

64.018

98.908

0.633
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631