Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   K8L98_RS17560 Genome accession   NZ_CP082944
Coordinates   3542196..3543263 (-) Length   355 a.a.
NCBI ID   WP_223436682.1    Uniprot ID   -
Organism   Metabacillus dongyingensis strain BY2G20     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3537196..3548263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8L98_RS17505 (K8L98_17495) - 3537280..3537501 (-) 222 WP_223436664.1 hypothetical protein -
  K8L98_RS17510 (K8L98_17500) - 3537643..3537909 (+) 267 WP_420828874.1 DUF3889 domain-containing protein -
  K8L98_RS17515 (K8L98_17505) - 3538001..3538309 (-) 309 Protein_3446 tyrosine-protein phosphatase -
  K8L98_RS17520 (K8L98_17510) - 3538499..3538678 (-) 180 WP_223436668.1 YqzE family protein -
  K8L98_RS17525 (K8L98_17515) - 3538703..3539221 (-) 519 WP_223436670.1 shikimate kinase -
  K8L98_RS17530 (K8L98_17520) comGG 3539297..3539671 (-) 375 WP_223436671.1 competence type IV pilus minor pilin ComGG -
  K8L98_RS17535 (K8L98_17525) comGF 3539668..3540165 (-) 498 WP_223436673.1 competence type IV pilus minor pilin ComGF -
  K8L98_RS17540 (K8L98_17530) comGE 3540092..3540421 (-) 330 WP_223436674.1 competence type IV pilus minor pilin ComGE -
  K8L98_RS17545 (K8L98_17535) comGD 3540408..3540860 (-) 453 WP_223436676.1 competence type IV pilus minor pilin ComGD -
  K8L98_RS17550 (K8L98_17540) comGC 3540838..3541146 (-) 309 WP_223436678.1 competence type IV pilus major pilin ComGC Machinery gene
  K8L98_RS17555 (K8L98_17545) comGB 3541172..3542206 (-) 1035 WP_223436680.1 competence type IV pilus assembly protein ComGB -
  K8L98_RS17560 (K8L98_17550) comGA 3542196..3543263 (-) 1068 WP_223436682.1 competence type IV pilus ATPase ComGA Machinery gene
  K8L98_RS17570 (K8L98_17560) - 3543575..3543823 (-) 249 WP_223436684.1 hypothetical protein -
  K8L98_RS17575 (K8L98_17565) - 3544011..3544391 (-) 381 WP_223436686.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  K8L98_RS17580 (K8L98_17570) - 3544662..3544907 (+) 246 WP_070876617.1 DUF2626 domain-containing protein -
  K8L98_RS17585 (K8L98_17575) - 3544950..3546041 (-) 1092 WP_223436688.1 class I SAM-dependent methyltransferase -
  K8L98_RS17590 (K8L98_17580) - 3546031..3546663 (-) 633 WP_223436689.1 MBL fold metallo-hydrolase -
  K8L98_RS17595 (K8L98_17585) - 3546747..3547211 (+) 465 WP_223436691.1 hypothetical protein -
  K8L98_RS17600 (K8L98_17590) - 3547296..3547469 (+) 174 WP_223436693.1 DUF2759 domain-containing protein -
  K8L98_RS17605 (K8L98_17595) - 3547702..3548013 (-) 312 WP_223436695.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 40181.46 Da        Isoelectric Point: 7.0403

>NTDB_id=603754 K8L98_RS17560 WP_223436682.1 3542196..3543263(-) (comGA) [Metabacillus dongyingensis strain BY2G20]
MNSIELLSERIVEEACSLNASDIHFVPRERDAVIQYRIDDDLVENRTIRKEVCERIIAHFKFLASMDIGERRRPQNGALA
LSNETIDVQLRLSTLPTIFEESLVIRILPNEVIPSLAHLSLFPQSANKLLSFINHSHGLMVFTGPTGSGKTTTLYSLIHY
AKKNFNRSIITLEDPVETKSSEVLQVQVNEKAGITYAVGLKAILRHDPDIIMVGEIRDRETAQIAIRAALTGHLVLSTMH
TRDAKGAIYRMLEFDVSLSEIEQTFLAIVAQRLVAMKCPYCSNECSPHCKKMRKSRRLGVFEMLYGKNLTLALKEAQGEE
VDCDYITLKDVIKKGIALGYLSNQSMHKWIYQYED

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=603754 K8L98_RS17560 WP_223436682.1 3542196..3543263(-) (comGA) [Metabacillus dongyingensis strain BY2G20]
ATGAATTCAATCGAACTACTAAGTGAAAGAATTGTGGAAGAAGCGTGTTCATTAAATGCATCAGATATCCATTTTGTCCC
GAGAGAACGGGATGCGGTCATTCAATATCGTATAGATGATGACCTCGTGGAAAATCGAACCATTAGAAAAGAAGTATGTG
AACGGATCATCGCACATTTTAAATTTTTGGCATCAATGGATATCGGAGAAAGAAGGAGGCCGCAAAATGGAGCATTAGCC
CTTTCAAATGAAACAATTGATGTTCAGCTTCGCTTGTCTACCCTGCCAACAATCTTTGAAGAAAGTCTTGTTATCCGCAT
ACTTCCAAACGAAGTCATTCCGTCACTTGCGCACCTCTCCCTGTTTCCACAGTCAGCAAATAAGCTTCTCTCCTTTATCA
ACCACTCTCATGGTCTGATGGTATTTACCGGTCCGACCGGCTCAGGAAAAACGACCACCTTATATTCATTGATTCATTAT
GCGAAAAAAAATTTTAACCGCAGTATTATTACCCTTGAAGATCCTGTAGAAACGAAAAGCTCTGAAGTCCTGCAGGTACA
GGTAAATGAAAAGGCTGGAATTACTTATGCAGTGGGGTTAAAAGCCATTCTAAGGCATGATCCGGACATTATAATGGTAG
GTGAAATCAGGGACAGGGAAACTGCGCAAATTGCCATAAGAGCAGCTCTGACAGGACACTTGGTGTTATCAACTATGCAT
ACAAGAGATGCAAAGGGAGCAATTTACAGAATGCTTGAATTTGATGTCAGTCTTTCTGAAATTGAACAAACATTTTTAGC
TATTGTGGCACAAAGGCTTGTAGCAATGAAATGCCCTTATTGCTCAAATGAATGCTCTCCTCACTGCAAAAAGATGAGAA
AGTCCAGACGTCTTGGAGTTTTCGAGATGCTTTATGGAAAAAATCTGACCCTTGCCTTAAAAGAAGCCCAGGGAGAGGAA
GTGGATTGTGACTATATAACCTTAAAAGATGTTATTAAAAAAGGAATTGCTTTAGGCTATTTATCCAATCAGTCAATGCA
TAAATGGATTTATCAATATGAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

61.517

100

0.617