Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   K8L98_RS00605 Genome accession   NZ_CP082944
Coordinates   113663..115045 (+) Length   460 a.a.
NCBI ID   WP_223438918.1    Uniprot ID   -
Organism   Metabacillus dongyingensis strain BY2G20     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 108663..120045
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8L98_RS00585 (K8L98_00585) - 108975..109436 (+) 462 WP_223438914.1 CtsR family transcriptional regulator -
  K8L98_RS00590 (K8L98_00590) - 109453..109998 (+) 546 WP_223438915.1 UvrB/UvrC motif-containing protein -
  K8L98_RS00595 (K8L98_00595) - 110000..111091 (+) 1092 WP_223438916.1 protein arginine kinase -
  K8L98_RS00600 (K8L98_00600) clpC 111088..113535 (+) 2448 WP_223438917.1 ATP-dependent protease ATP-binding subunit ClpC -
  K8L98_RS00605 (K8L98_00605) radA 113663..115045 (+) 1383 WP_223438918.1 DNA repair protein RadA Machinery gene
  K8L98_RS00610 (K8L98_00610) disA 115050..116132 (+) 1083 WP_223438919.1 DNA integrity scanning diadenylate cyclase DisA -
  K8L98_RS00615 (K8L98_00615) - 116326..117426 (+) 1101 WP_223438920.1 PIN/TRAM domain-containing protein -
  K8L98_RS00620 (K8L98_00620) ispD 117439..118125 (+) 687 WP_223438921.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  K8L98_RS00625 (K8L98_00625) ispF 118125..118601 (+) 477 WP_223438922.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49644.15 Da        Isoelectric Point: 7.3511

>NTDB_id=603727 K8L98_RS00605 WP_223438918.1 113663..115045(+) (radA) [Metabacillus dongyingensis strain BY2G20]
MAKTKSKFMCLTCGYESAKWMGKCPGCGAWNTMTEEIVKTGTANRRVAFAHSAPGQTANKPSPITNIETTQEPRIKTNLQ
EFNRVLGSGIVRGSLVLIGGDPGIGKSTLLLQVSSQLADNGNDVLYISGEESVKQTKMRADRLGVKSEKLYVLAETDLEF
ISKAIDETNPAFVIVDSIQTVFQSDITSAPGSVSQVRECTAELMRLAKTKGIAIFIVGHVTKEGSIAGPRILEHMVDTVL
YFEGERHHTYRILRAVKNRFGSTNEMGIFEMKESGLEEVLNPSEIFLEERSKGAAGSTVVASMEGTRPVLVEIQALISPT
SFGNPRRMATGIDHNRVPLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAVSIASSFKDVPSKATDIIIGEVG
LTGEVRRVSRIEQRVMEAAKLGFKRAIVPEANIGGWTVPGDIEVVGVKNVSEALQTTLGG

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=603727 K8L98_RS00605 WP_223438918.1 113663..115045(+) (radA) [Metabacillus dongyingensis strain BY2G20]
ATGGCTAAAACAAAATCGAAATTCATGTGTCTGACGTGCGGATATGAATCTGCCAAATGGATGGGGAAATGTCCAGGCTG
CGGCGCATGGAACACGATGACAGAGGAAATTGTCAAAACAGGAACTGCAAATAGAAGAGTTGCCTTTGCGCACTCAGCTC
CAGGTCAAACCGCCAATAAACCTTCACCCATTACAAACATTGAAACAACTCAAGAACCGCGCATTAAAACAAACCTGCAG
GAATTTAACCGTGTCCTTGGAAGCGGAATTGTAAGGGGCTCCTTAGTGTTAATAGGAGGAGATCCTGGTATAGGAAAATC
AACACTTCTGCTTCAAGTTTCTTCTCAGTTAGCCGACAATGGAAACGATGTCTTATATATTTCAGGCGAAGAATCGGTCA
AGCAAACGAAAATGAGGGCTGACAGACTTGGTGTTAAATCTGAAAAGCTCTATGTACTTGCTGAAACAGATCTTGAATTT
ATTTCAAAAGCGATTGATGAAACGAATCCTGCATTTGTAATCGTGGATTCGATCCAAACGGTTTTTCAAAGTGACATTAC
ATCTGCACCGGGGAGTGTTTCACAAGTGCGGGAATGTACTGCTGAACTGATGAGACTCGCGAAAACAAAAGGCATTGCGA
TCTTTATTGTGGGACATGTCACGAAGGAAGGGTCCATTGCAGGTCCGAGAATCCTAGAACACATGGTTGATACCGTGCTT
TATTTTGAAGGAGAACGTCATCATACGTACCGGATTTTAAGGGCGGTGAAAAACCGCTTTGGCTCTACAAATGAAATGGG
TATTTTTGAAATGAAAGAAAGCGGTCTTGAGGAGGTACTGAACCCTTCAGAGATTTTTCTTGAGGAACGTTCAAAAGGTG
CAGCTGGCTCAACGGTTGTTGCATCGATGGAAGGAACAAGGCCGGTTCTTGTGGAAATTCAGGCGTTAATCTCGCCTACA
AGCTTCGGGAATCCGCGAAGAATGGCAACCGGGATTGACCATAATCGTGTACCGCTGTTAATGGCTGTCCTTGAGAAAAG
AGTTGGCTTGCTTCTGCAAAACCAGGATGCCTATTTAAAAGTGGCAGGAGGAGTGAAATTAGATGAACCTGCTATTGATT
TAGCAATAGCAGTCAGCATAGCTTCAAGCTTTAAGGACGTACCATCAAAAGCGACGGATATCATTATTGGAGAAGTAGGC
TTAACAGGCGAAGTCAGACGCGTTTCAAGAATCGAACAGCGCGTCATGGAAGCAGCCAAGCTTGGGTTTAAGCGTGCAAT
CGTTCCAGAGGCCAACATTGGGGGATGGACGGTGCCGGGGGACATCGAGGTTGTGGGAGTAAAGAACGTCTCAGAGGCCC
TGCAGACTACATTAGGGGGGTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(66-224)

Subunit ChlI of Mg-chelatase (ChlI)

(367-432)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

81.087

100

0.811
  radA Streptococcus pneumoniae Rx1

60.879

98.913

0.602
  radA Streptococcus pneumoniae D39

60.879

98.913

0.602
  radA Streptococcus pneumoniae R6

60.879

98.913

0.602
  radA Streptococcus pneumoniae TIGR4

60.879

98.913

0.602
  radA Streptococcus mitis SK321

60.879

98.913

0.602
  radA Streptococcus mitis NCTC 12261

60.879

98.913

0.602