Detailed information NTG0603632

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: NI384_RS13610
• Genome accession: NZ_CP099928
• Coordinates: 2944972..2947176 (+)
• Length: 734 a.a.
• NCBI ID: WP_308385304.1
• Uniprot ID: -
• Organism: Vibrio parahaemolyticus strain BM24B
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2939972..2952176

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NI384_RS13570 (NI384_13665)	rplV	2940641..2940973 (-)	333	WP_005383164.1	50S ribosomal protein L22	-
NI384_RS13575 (NI384_13670)	rpsS	2940984..2941262 (-)	279	WP_004394525.1	30S ribosomal protein S19	-
NI384_RS13580 (NI384_13675)	rplB	2941284..2942108 (-)	825	WP_005489461.1	50S ribosomal protein L2	-
NI384_RS13585 (NI384_13680)	rplW	2942124..2942426 (-)	303	WP_004398471.1	50S ribosomal protein L23	-
NI384_RS13590 (NI384_13685)	rplD	2942423..2943025 (-)	603	WP_005379556.1	50S ribosomal protein L4	-
NI384_RS13595 (NI384_13690)	rplC	2943043..2943672 (-)	630	WP_005456132.1	50S ribosomal protein L3	-
NI384_RS13600 (NI384_13695)	rpsJ	2943687..2943998 (-)	312	WP_004410492.1	30S ribosomal protein S10	-
NI384_RS13605 (NI384_13700)	rpmE	2944456..2944677 (-)	222	WP_005457203.1	50S ribosomal protein L31	-
NI384_RS13610 (NI384_13705)	priA	2944972..2947176 (+)	2205	WP_308385304.1	primosomal protein N'	Machinery gene
NI384_RS13615 (NI384_13710)	cytR	2947509..2948516 (+)	1008	WP_005481416.1	DNA-binding transcriptional regulator CytR	Regulator
NI384_RS13620 (NI384_13715)	ftsN	2948692..2949237 (+)	546	WP_005481405.1	cell division protein FtsN	-
NI384_RS13625 (NI384_13720)	hslV	2949401..2949952 (+)	552	WP_005489705.1	ATP-dependent protease subunit HslV	-
NI384_RS13630 (NI384_13725)	hslU	2949976..2951307 (+)	1332	WP_005457198.1	HslU--HslV peptidase ATPase subunit	-

Sequence

Protein

• Length: 734 a.a.
• Molecular weight: 82354.97 Da
• Isoelectric Point: 8.5723

>NTDB_id=603632 NI384_RS13610 WP_308385304.1 2944972..2947176(+) (priA) [Vibrio parahaemolyticus strain BM24B]
MRPSIARVALPVPLDKQFDYAIPGHLFPIIGGRVSVPFGRQTLVGIVTAMVNHSDFPKDQLKPIKAVLDFQPIWPEKLYS
LLTWCSQFYQYPLGDTLHNAMPAALRKGKPADFATLQEWQITESGKDKLMQGLDRRAVKQQKVLQMLVNGALPHQEFVDQ
EIASTVLKSLEEKGWIERIEKKPAITQWGQHVECDVEKPKLNHEQALAIASVNSQTGFACYLLEGVTGSGKTEVYLNLIK
PVLEKGKQALVLVPEIGLTPQTINRFKRRFNVPVDVIHSGLNETERLNAWLSARDKAAGIIIGTRSALLAPFADLGIIIV
DEEHDTSYKQQDSLRYHARDVAVMRAHKEQVPIVLGSATPALETLHNALSGKYHHLTLTQRAGSAVPTTNKVLDVKGQYL
ESGLSAPLIAEMRKHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTFHQYSNEIRCHHCGSQHPVIHQCQGC
GSTQLVTVGVGTEQLEQQLVQLFPEYKAIRIDRDSTRRKGSLEDALDSIRKGEYQILIGTQMLAKGHHFPNVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLEKDYRHFAMTALEERKLAQLPPYSFLTLF
KAEANQSEIVEDFLRQVRFTLESHPLFDDTCMVLGPTPSPLAKRAGKYRWQLLLQTQHRSLMQKLLTSAKPAIELLPNAK
KVRWNLDIEPQDLS

Nucleotide

• Length: 2205 bp

>NTDB_id=603632 NI384_RS13610 WP_308385304.1 2944972..2947176(+) (priA) [Vibrio parahaemolyticus strain BM24B]
ATGCGTCCATCCATTGCTAGAGTGGCACTGCCCGTACCTCTCGACAAACAATTTGACTATGCCATCCCAGGACACCTGTT
TCCTATCATTGGCGGGCGCGTATCCGTACCTTTTGGGCGTCAAACGTTAGTTGGCATCGTCACCGCGATGGTTAATCACT
CGGATTTCCCCAAAGATCAACTCAAGCCAATCAAAGCGGTTCTTGATTTTCAGCCGATATGGCCTGAAAAGCTTTATTCG
TTGCTGACTTGGTGCAGCCAATTCTACCAGTACCCACTTGGAGATACGCTACACAATGCGATGCCGGCAGCTCTGCGTAA
AGGAAAGCCTGCAGATTTTGCGACGCTACAAGAGTGGCAAATTACTGAGTCCGGTAAAGACAAGCTGATGCAAGGGCTTG
ATCGTCGAGCGGTCAAACAACAGAAGGTCTTGCAAATGTTGGTTAACGGCGCTTTGCCTCACCAGGAATTTGTCGACCAA
GAGATAGCCTCAACTGTACTAAAGTCTTTAGAAGAGAAAGGCTGGATTGAACGTATCGAGAAGAAGCCTGCAATCACCCA
GTGGGGACAGCACGTTGAATGCGACGTCGAGAAGCCAAAGCTCAACCACGAACAAGCGCTGGCTATTGCGAGTGTAAATA
GCCAAACAGGTTTTGCTTGCTATTTATTAGAAGGCGTCACGGGCTCAGGTAAGACAGAAGTGTACCTTAACCTGATCAAA
CCGGTCTTAGAGAAAGGTAAACAAGCCTTGGTTTTAGTACCAGAGATTGGCTTAACACCACAAACCATCAACCGCTTTAA
ACGCCGTTTTAATGTGCCAGTTGACGTTATCCACTCCGGGTTAAACGAGACGGAGCGTCTGAATGCATGGCTTTCTGCAC
GCGACAAAGCGGCCGGAATCATTATCGGCACACGCTCTGCCCTACTCGCGCCTTTTGCTGATCTCGGGATTATCATTGTG
GACGAGGAACATGACACCTCTTACAAACAGCAAGATAGCTTGCGTTATCATGCACGCGATGTTGCTGTAATGCGCGCTCA
TAAAGAACAAGTTCCGATTGTTCTGGGCTCAGCTACACCTGCACTGGAGACATTGCACAATGCCTTGTCAGGCAAGTATC
ACCATTTAACCCTGACCCAACGCGCAGGCTCTGCGGTTCCTACTACTAATAAAGTGCTCGACGTCAAAGGTCAGTATTTA
GAAAGTGGTTTATCTGCCCCATTGATTGCGGAAATGCGCAAACACCTTAAAGCAGGCAATCAGGTGATGCTGTTTCTTAA
CCGTCGTGGTTTCTCCCCTGCTCTCATGTGCCATGAATGTGGTTGGATTGCCGAATGCAAACGTTGTGATGCTTATTACA
CCTTCCACCAATACAGCAATGAGATTCGCTGCCACCATTGTGGTTCACAGCACCCAGTGATTCATCAGTGTCAGGGCTGT
GGTTCAACTCAACTGGTTACCGTTGGTGTAGGTACCGAACAGTTGGAACAACAATTGGTACAACTGTTCCCCGAATACAA
AGCCATCCGTATCGACAGAGATAGCACGCGCCGCAAAGGCAGTTTAGAAGATGCGCTGGACTCCATCCGCAAGGGTGAAT
ATCAAATTTTAATTGGCACTCAAATGTTAGCTAAGGGACACCATTTCCCCAATGTAACGCTCGTTGCCTTGCTAGATGTT
GATGGCTCTCTATATAGTAGCGACTTCCGCGCCTCCGAAAGATTAGCGCAGTTATTCATTCAAGTCGCAGGGCGAGCCGG
ACGTGCAAGTAAGCCGGGGGAAGTGGTTTTGCAGACTCACCATCCTGAGCACAGTTTGTTGCAAGCCTTATTGGAAAAGG
ACTACCGACACTTCGCTATGACGGCATTAGAGGAACGTAAACTGGCGCAGTTGCCACCGTACAGTTTCCTTACTCTATTT
AAAGCCGAAGCGAATCAGAGCGAGATCGTAGAAGATTTTCTGCGCCAAGTTCGCTTTACCTTAGAATCGCACCCTTTATT
TGACGACACCTGCATGGTGCTTGGTCCAACACCATCGCCTCTCGCCAAACGCGCAGGCAAATACCGTTGGCAACTCTTGC
TGCAAACCCAACACCGGTCATTAATGCAAAAGTTATTAACCAGTGCCAAACCTGCTATTGAGTTGTTACCTAATGCCAAG
AAAGTACGTTGGAATTTAGACATAGAACCACAAGATCTCAGCTAG

Domains

Predicted by InterProScan.

• DEAD/DEAH box helicase (DEAD) (220-365)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (446-472)

• Helicase conserved C-terminal domain (Helicase_C) (500-589)

• Primosomal protein N C-terminal domain (PriA_C) (633-730)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (7-102)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	43.784	100	0.441
priA	Bacillus subtilis subsp. subtilis str. 168	34.005	100	0.368