Detailed information NTG0603621

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: K8U58_RS20200
• Genome accession: NZ_CP082924
• Coordinates: 3845621..3846766 (+)
• Length: 381 a.a.
• NCBI ID: WP_003227549.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain FJAT-4
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3840621..3851766

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K8U58_RS20180 (K8U58_20110)	albF	3840637..3841917 (+)	1281	WP_003242974.1	M16 family metallopeptidase	-
K8U58_RS20185 (K8U58_20115)	albG	3841914..3842615 (+)	702	WP_003243391.1	antilisterial bacteriocin subtilosin biosynthesis protein AlbG	-
K8U58_RS20190 (K8U58_20120)	ywhL	3842621..3843997 (-)	1377	WP_003244415.1	YncE family protein	-
K8U58_RS20195 (K8U58_20125)	ywhK	3844036..3845391 (-)	1356	WP_003227552.1	YncE family protein	-
K8U58_RS22700	-	3845388..3845618 (-)	231	WP_009968328.1	hypothetical protein	-
K8U58_RS20200 (K8U58_20130)	rapF	3845621..3846766 (+)	1146	WP_003227549.1	response regulator aspartate phosphatase RapF	Regulator
K8U58_RS20205 (K8U58_20135)	phrF	3846750..3846869 (+)	120	WP_009968329.1	phosphatase RapF inhibitor PhrF	Regulator
K8U58_RS20210 (K8U58_20140)	ywhH	3846968..3847441 (+)	474	WP_003227547.1	YbaK/EbsC family protein	-
K8U58_RS20215 (K8U58_20145)	speB	3847473..3848345 (-)	873	WP_003227545.1	agmatinase	-
K8U58_RS20220 (K8U58_20150)	speE	3848406..3849236 (-)	831	WP_003227543.1	spermidine synthase	-
K8U58_RS20225 (K8U58_20155)	pbpG	3849438..3851513 (+)	2076	WP_003227540.1	transglycosylase domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45609.09 Da
• Isoelectric Point: 5.1503

>NTDB_id=603621 K8U58_RS20200 WP_003227549.1 3845621..3846766(+) (rapF) [Bacillus subtilis strain FJAT-4]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKEHEAYNIRLLQCHSLFATNFLDLKQYEDAISHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AIPYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKIDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFAIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=603621 K8U58_RS20200 WP_003227549.1 3845621..3846766(+) (rapF) [Bacillus subtilis strain FJAT-4]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTAATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAACTTCTTCAG
AGGCATGTACGAGCTGGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAACATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCATCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGGAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATACCTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATAGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (223-290)

• Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3) (132-208)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	100	100	1
rapC	Bacillus subtilis subsp. subtilis str. 168	57.368	99.738	0.572