Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   K8U58_RS13395 Genome accession   NZ_CP082924
Coordinates   2557572..2558609 (-) Length   345 a.a.
NCBI ID   WP_009967727.1    Uniprot ID   A0AAE2SM96
Organism   Bacillus subtilis strain FJAT-4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2552572..2563609
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8U58_RS13345 (K8U58_13310) tasA 2552670..2553455 (-) 786 WP_004398632.1 biofilm matrix protein TasA -
  K8U58_RS13350 (K8U58_13315) sipW 2553519..2554091 (-) 573 WP_003246088.1 signal peptidase I SipW -
  K8U58_RS13355 (K8U58_13320) tapA 2554075..2554836 (-) 762 WP_004399106.1 amyloid fiber anchoring/assembly protein TapA -
  K8U58_RS13360 (K8U58_13325) yqzG 2555108..2555434 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  K8U58_RS13365 (K8U58_13330) spoIITA 2555476..2555655 (-) 180 WP_003230176.1 YqzE family protein -
  K8U58_RS13370 (K8U58_13335) comGG 2555726..2556100 (-) 375 WP_003230170.1 ComG operon protein ComGG Machinery gene
  K8U58_RS13375 (K8U58_13340) comGF 2556101..2556484 (-) 384 WP_003230168.1 ComG operon protein ComGF Machinery gene
  K8U58_RS13380 (K8U58_13345) comGE 2556510..2556857 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  K8U58_RS13385 (K8U58_13350) comGD 2556841..2557272 (-) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  K8U58_RS13390 (K8U58_13355) comGC 2557262..2557558 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  K8U58_RS13395 (K8U58_13360) comGB 2557572..2558609 (-) 1038 WP_009967727.1 comG operon protein ComGB Machinery gene
  K8U58_RS13400 (K8U58_13365) comGA 2558596..2559666 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  K8U58_RS13405 (K8U58_13370) corA 2560078..2561031 (-) 954 WP_004399136.1 magnesium transporter CorA -
  K8U58_RS13410 (K8U58_13375) yqhB 2561174..2562502 (+) 1329 WP_009967730.1 hemolysin family protein -
  K8U58_RS13415 (K8U58_13380) rsbRD 2562555..2563391 (-) 837 WP_004398754.1 RsbT co-antagonist protein RsbRD -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39762.26 Da        Isoelectric Point: 9.6257

>NTDB_id=603592 K8U58_RS13395 WP_009967727.1 2557572..2558609(-) (comGB) [Bacillus subtilis strain FJAT-4]
MKKIRKVWLLKDQARLLKRLGEMTAGGYTLLDGLRLMELQMNKRQAADLTDSVTCLREGAPFYQVLKSLSFHKEAVGICY
FAETHGELPASMIQSGELLERKIAQADQLKRVLRYPLFLIFTVAVMFYMLQSIIIPQFSGIYQSMNMETSRSTDMLFAFF
QHIDLVIILLVLFTAGIGIYYWLVFKKKSPARQMLICIRIPLVGKLVKLFNSYFFSLQLSSLLKSGLSIYDSLNAFKHQT
FLPFYRCEAEQLIERLKAGESIESAICGSLFYETDLSKVISHGQLSGRLDRELFTYSQFILQRLEHKAQKWTGILQPMIY
GFVAAMILLVYLSMLVPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=603592 K8U58_RS13395 WP_009967727.1 2557572..2558609(-) (comGB) [Bacillus subtilis strain FJAT-4]
ATGAAAAAGATTAGAAAAGTCTGGTTGTTAAAGGATCAAGCCAGGTTATTAAAGAGGCTCGGTGAAATGACTGCGGGCGG
ATATACACTTCTGGATGGATTACGCCTGATGGAACTTCAGATGAATAAGAGGCAGGCGGCTGACTTGACTGATTCGGTCA
CTTGTTTGAGGGAAGGGGCTCCGTTTTATCAAGTACTAAAGAGTTTGTCATTTCATAAGGAAGCCGTAGGTATTTGTTAT
TTTGCTGAAACACATGGTGAACTGCCTGCTTCAATGATCCAGAGCGGAGAGCTGCTGGAACGAAAAATTGCACAGGCAGA
CCAGCTGAAAAGAGTGCTGCGCTATCCGCTTTTCCTCATCTTTACGGTCGCTGTCATGTTTTATATGTTACAGTCCATCA
TCATTCCTCAGTTTTCCGGTATCTATCAATCGATGAATATGGAAACCTCACGTTCAACCGATATGCTTTTTGCTTTTTTT
CAGCATATTGATCTTGTGATCATTTTGCTTGTTCTTTTTACAGCAGGTATCGGGATTTATTATTGGCTTGTGTTTAAGAA
AAAATCACCTGCCCGGCAAATGCTGATTTGTATCAGGATTCCTTTGGTTGGAAAGCTTGTAAAGCTGTTTAACAGCTACT
TTTTTTCTTTGCAGCTAAGCAGCCTTTTAAAATCAGGCCTCTCAATTTATGACAGCCTTAATGCATTTAAACATCAAACG
TTTCTCCCTTTCTACCGCTGCGAGGCTGAACAATTGATTGAACGGCTAAAAGCCGGTGAGTCAATTGAATCCGCTATTTG
TGGAAGCCTTTTTTATGAAACTGATTTATCAAAAGTCATATCTCACGGCCAGCTGAGCGGCCGATTGGATCGGGAGCTTT
TCACATACAGCCAATTCATATTACAGCGGCTGGAACACAAAGCGCAAAAATGGACAGGCATCCTTCAGCCAATGATTTAT
GGATTTGTTGCAGCGATGATCTTACTTGTGTATTTATCTATGCTTGTGCCTATGTATCAGATGATGAATCAAATGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(17-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

100

93.623

0.936