Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   K6958_RS02490 Genome accession   NZ_CP082904
Coordinates   527868..529301 (-) Length   477 a.a.
NCBI ID   WP_249893183.1    Uniprot ID   -
Organism   Mixta hanseatica isolate X22927     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 522868..534301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6958_RS02465 (K6958_02460) mtgA 524963..525679 (+) 717 WP_249893179.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  K6958_RS02470 (K6958_02465) npr 525686..525958 (-) 273 WP_249893180.1 PTS phosphocarrier protein NPr -
  K6958_RS02475 (K6958_02470) rapZ 525955..526809 (-) 855 WP_249893181.1 RNase adapter RapZ -
  K6958_RS02480 (K6958_02475) ptsN 526881..527363 (-) 483 WP_249894572.1 PTS IIA-like nitrogen regulatory protein PtsN -
  K6958_RS02485 (K6958_02480) hpf 527557..527844 (-) 288 WP_249893182.1 ribosome hibernation promoting factor -
  K6958_RS02490 (K6958_02485) rpoN/rpoN1 527868..529301 (-) 1434 WP_249893183.1 RNA polymerase factor sigma-54 Machinery gene
  K6958_RS02495 (K6958_02490) lptB 529350..530075 (-) 726 WP_249893184.1 LPS export ABC transporter ATP-binding protein -
  K6958_RS02500 (K6958_02495) lptA 530081..530632 (-) 552 WP_249893185.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  K6958_RS02505 (K6958_02500) lptC 530604..531179 (-) 576 WP_249893186.1 LPS export ABC transporter periplasmic protein LptC -
  K6958_RS02510 (K6958_02505) kdsC 531176..531742 (-) 567 WP_249893187.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  K6958_RS02515 (K6958_02510) kdsD 531760..532746 (-) 987 WP_249893188.1 arabinose-5-phosphate isomerase KdsD -
  K6958_RS02520 (K6958_02515) - 532761..533741 (-) 981 WP_249893189.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53859.70 Da        Isoelectric Point: 4.4165

>NTDB_id=603405 K6958_RS02490 WP_249893183.1 527868..529301(-) (rpoN/rpoN1) [Mixta hanseatica isolate X22927]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQEIQLALESNPLLEQTDIHEEVDSRDYVESEALDTREALEQKDMPE
ELPLDATWDEIYTAGTPSGTGNDYRDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAIDDTGYLTVTLDE
IRDSIGDEDVALDEVEAVLKRVQRFDPVGVGARNLRDCLLVQLSQFARDTPFLAEAQLIVDQYLDLLANHDFRSLMRVTR
LKEDVLKTALALIQSLDPRPGQSVNTREPEYVIPDVLVRKTANRWTVELNGDSVPRLQINQQYAALGSSARSDSDSQFIR
TNLQDAKWLIKSLESRNDTLLKVTRCIVEQQQAFFEQGEEFMRPMVLADIAQAVDMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLISAENPAKPLSDSKLTTLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=603405 K6958_RS02490 WP_249893183.1 527868..529301(-) (rpoN/rpoN1) [Mixta hanseatica isolate X22927]
ATGAAGCAAGGTTTGCAACTCAGGCTCAGTCAACAGCTTGCGATGACTCCGCAGCTACAGCAGGCAATTCGTTTGCTGCA
GCTTTCTACGCTGGAACTTCAACAGGAGATACAGCTGGCGCTGGAGAGTAATCCGTTGCTTGAGCAGACCGATATCCACG
AGGAAGTCGACTCACGCGACTATGTCGAAAGCGAAGCGCTCGACACACGTGAGGCGTTGGAACAAAAGGATATGCCGGAA
GAGTTGCCGCTTGATGCAACCTGGGATGAGATCTATACCGCCGGTACGCCTTCCGGCACCGGTAATGATTACCGCGATGA
CGAACTACCGGTTTATCAGGGCGAAACTACCCAGACCTTGCAGGATTACCTGATGTGGCAGGTAGAGCTAACGCCGTTTA
GCGATACCGACCGCGCCATCGCCACCTCTATCGTCGACGCCATTGATGACACCGGTTATCTTACCGTTACGCTTGATGAG
ATCCGCGATAGCATCGGCGATGAAGATGTGGCGCTGGATGAGGTCGAAGCAGTACTCAAACGAGTGCAACGTTTCGATCC
GGTTGGCGTTGGTGCCCGTAACCTGCGCGACTGCCTACTGGTCCAGCTTTCTCAATTCGCGCGTGATACGCCCTTTTTGG
CTGAAGCGCAGCTGATTGTCGATCAGTACCTCGATCTGCTGGCGAACCATGATTTCCGCAGCCTGATGCGGGTGACGCGC
CTGAAAGAGGATGTGTTAAAAACCGCGCTGGCGCTGATTCAGTCGCTCGATCCGCGGCCGGGCCAGTCGGTGAACACCCG
CGAGCCGGAATATGTCATTCCTGATGTGCTGGTGCGCAAAACCGCCAACCGCTGGACCGTGGAGTTAAACGGCGACAGCG
TGCCGCGCCTGCAGATTAATCAGCAATACGCCGCGCTGGGTAGCTCTGCGCGTAGCGACAGCGATAGCCAGTTTATTCGT
ACCAATTTACAGGATGCGAAGTGGCTGATTAAAAGCCTGGAAAGCCGCAACGATACGCTATTGAAAGTAACGCGCTGTAT
CGTTGAGCAGCAGCAGGCTTTTTTTGAGCAGGGCGAAGAATTTATGCGCCCGATGGTGCTGGCGGATATCGCTCAGGCCG
TTGATATGCATGAATCCACCATTTCCCGCGTTACCACCCAAAAATATCTGCACAGCCCACGCGGCATTTTCGAACTGAAA
TACTTTTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAGGCCTCCTCAACCGCTATTCGCGCGTTGGTGAAAAAGCT
GATCTCGGCGGAAAACCCCGCCAAACCACTGAGCGACAGTAAGCTCACTACCCTGTTGTCTGAACAAGGCATTATGGTGG
CACGCCGTACCGTCGCGAAGTATCGCGAGTCTTTATCCATCCCGCCATCAAATCAGCGTAAACAATTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.924

100

0.484