Detailed information NTG0603375

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: NI388_RS14065
• Genome accession: NZ_CP099919
• Coordinates: 2991109..2993313 (+)
• Length: 734 a.a.
• NCBI ID: WP_308390417.1
• Uniprot ID: -
• Organism: Vibrio parahaemolyticus strain Isc14B
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2986109..2998313

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NI388_RS14025 (NI388_14670)	rplV	2986778..2987110 (-)	333	WP_005383164.1	50S ribosomal protein L22	-
NI388_RS14030 (NI388_14675)	rpsS	2987121..2987399 (-)	279	WP_004394525.1	30S ribosomal protein S19	-
NI388_RS14035 (NI388_14680)	rplB	2987421..2988245 (-)	825	WP_005489461.1	50S ribosomal protein L2	-
NI388_RS14040 (NI388_14685)	rplW	2988261..2988563 (-)	303	WP_004398471.1	50S ribosomal protein L23	-
NI388_RS14045 (NI388_14690)	rplD	2988560..2989162 (-)	603	WP_005379556.1	50S ribosomal protein L4	-
NI388_RS14050 (NI388_14695)	rplC	2989180..2989809 (-)	630	WP_005456132.1	50S ribosomal protein L3	-
NI388_RS14055 (NI388_14700)	rpsJ	2989824..2990135 (-)	312	WP_004410492.1	30S ribosomal protein S10	-
NI388_RS14060 (NI388_14705)	rpmE	2990593..2990814 (-)	222	WP_005457203.1	50S ribosomal protein L31	-
NI388_RS14065 (NI388_14710)	priA	2991109..2993313 (+)	2205	WP_308390417.1	primosomal protein N'	Machinery gene
NI388_RS14070 (NI388_14715)	cytR	2993646..2994653 (+)	1008	WP_005481416.1	DNA-binding transcriptional regulator CytR	Regulator
NI388_RS14075 (NI388_14720)	ftsN	2994830..2995375 (+)	546	WP_005481405.1	cell division protein FtsN	-
NI388_RS14080 (NI388_14725)	hslV	2995539..2996090 (+)	552	WP_005489705.1	ATP-dependent protease subunit HslV	-
NI388_RS14085 (NI388_14730)	hslU	2996114..2997445 (+)	1332	WP_308390418.1	HslU--HslV peptidase ATPase subunit	-

Sequence

Protein

• Length: 734 a.a.
• Molecular weight: 82322.00 Da
• Isoelectric Point: 8.6765

>NTDB_id=603375 NI388_RS14065 WP_308390417.1 2991109..2993313(+) (priA) [Vibrio parahaemolyticus strain Isc14B]
MRPSIARVALPVPLDKQFDYAIPGHLFPIIGGRVSVPFGRQTLVGIVTAMVNHSDFPKDQLKPIKAVLDSQPVWPEKLYS
LLIWCSQFYQYPLGDTLHNAMPAALRKGKPADFATLQEWQITESGKDKLMQGLDRRAIKQQKVLQMLVNGALPHQEFVDQ
EIASTVLKSLEEKGWIERIEKKPVITKWGQHVECDVEKPKLNHEQALAIASVNSQTGFACYLLEGVTGSGKTEVYLNLIK
PVLEKGKQALVLVPEIGLTPQTINRFKRRFNVPVDVIHSGLNETERLNAWLSARDKAAGIIIGTRSALLAPFADLGIIIV
DEEHDTSYKQQDSLRYHARDVAVMRAHKEQVPIVLGSATPALETLHNALSGKYHHLTLTQRAGSAVPTTNKVLDVKGQYL
ESGLSAPLIAEMRKHLKVGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTFHQYSNEIRCHHCGSQQPVIHQCQGC
GSTQLVTVGVGTEQLEQQLAQLFPEYKAIRIDRDSTRRKGSLEDALESIRKGEYQILIGTQMLAKGHHFPNVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLEKDYRHFAMTALEERKLAQLPPYSFLTLF
KAEANQSEIVEDFLRQVRFTLESHPLFDDTCIVLGPTPSPLAKRAGKYRWQLLLQTQHRSLMQKLLTSAKPAIELLPNAK
KVRWNLDIEPQDLS

Nucleotide

• Length: 2205 bp

>NTDB_id=603375 NI388_RS14065 WP_308390417.1 2991109..2993313(+) (priA) [Vibrio parahaemolyticus strain Isc14B]
ATGCGTCCATCCATTGCTAGAGTGGCACTGCCCGTACCTCTCGACAAACAATTTGACTATGCCATCCCAGGACACCTGTT
TCCTATCATTGGCGGGCGCGTATCCGTACCTTTTGGACGTCAAACGTTAGTTGGCATCGTCACCGCGATGGTTAATCACT
CGGATTTCCCCAAAGATCAACTCAAGCCAATCAAAGCAGTTCTTGATTCTCAGCCGGTATGGCCTGAAAAGCTTTATTCG
TTGCTGATTTGGTGCAGCCAGTTCTACCAATACCCACTTGGAGATACGCTACACAATGCGATGCCAGCAGCTCTGCGTAA
AGGAAAGCCTGCAGATTTTGCGACGCTACAAGAGTGGCAAATTACCGAATCCGGTAAAGACAAGCTGATGCAAGGGCTTG
ATCGTCGAGCGATCAAACAACAGAAGGTCTTGCAAATGTTGGTTAACGGCGCTTTGCCTCACCAGGAATTTGTCGACCAA
GAGATAGCCTCAACTGTACTAAAGTCTTTAGAAGAGAAAGGCTGGATTGAACGTATCGAGAAGAAGCCTGTAATCACCAA
GTGGGGGCAGCACGTTGAATGCGACGTCGAGAAGCCAAAGCTCAACCACGAACAAGCGCTAGCTATTGCGAGCGTAAATA
GCCAAACAGGTTTTGCCTGCTATTTATTAGAAGGCGTCACGGGCTCAGGTAAGACAGAAGTGTACCTCAACCTGATCAAA
CCGGTCTTAGAGAAAGGTAAACAAGCTTTGGTTTTAGTACCAGAGATTGGCTTAACACCGCAAACCATCAACCGCTTTAA
ACGCCGTTTTAATGTGCCAGTTGACGTTATCCACTCCGGATTAAACGAGACGGAGCGTCTGAATGCGTGGCTTTCTGCAC
GCGACAAAGCGGCCGGAATCATTATCGGCACACGCTCTGCCCTACTCGCGCCTTTTGCTGATCTCGGGATTATCATTGTG
GACGAGGAACATGACACCTCTTACAAACAGCAAGATAGCTTGCGTTATCATGCACGCGATGTTGCTGTAATGCGCGCTCA
TAAAGAACAAGTTCCGATTGTTCTGGGCTCAGCCACACCTGCACTGGAGACATTGCACAATGCCTTGTCAGGCAAGTATC
ACCATTTAACCCTGACCCAACGCGCAGGCTCTGCGGTTCCTACTACTAATAAAGTTCTCGACGTCAAAGGTCAGTATTTA
GAAAGTGGTTTATCTGCCCCATTGATTGCGGAAATGCGCAAACACCTTAAAGTAGGCAATCAGGTGATGCTGTTTCTTAA
CCGTCGTGGTTTCTCCCCTGCTCTCATGTGCCATGAATGTGGTTGGATTGCCGAATGCAAACGTTGTGATGCTTATTACA
CCTTCCACCAATACAGCAATGAGATTCGCTGCCACCATTGTGGCTCGCAGCAACCAGTGATTCATCAGTGTCAGGGCTGT
GGTTCAACTCAACTGGTTACCGTTGGTGTCGGTACCGAACAGTTAGAACAACAATTGGCACAACTGTTCCCCGAATACAA
AGCCATCCGTATCGACAGGGATAGCACGCGCCGAAAAGGCAGCTTAGAAGACGCGTTGGAATCCATCCGTAAGGGTGAAT
ATCAAATTTTGATTGGCACTCAAATGTTGGCTAAGGGACACCATTTCCCCAATGTAACGCTCGTCGCCTTGCTAGATGTT
GATGGCTCTCTATATAGTAGCGACTTTCGCGCCTCCGAAAGATTAGCGCAGCTATTCATTCAGGTCGCAGGGCGAGCCGG
ACGTGCAAGTAAGCCGGGGGAAGTGGTTTTGCAGACTCACCATCCTGAGCACAGTTTGTTGCAAGCCTTATTGGAAAAGG
ACTACCGACACTTCGCTATGACGGCATTAGAGGAACGTAAACTGGCGCAGTTGCCACCGTACAGTTTCCTTACTCTATTT
AAAGCCGAAGCGAATCAGAGCGAGATCGTAGAAGATTTTCTGCGTCAAGTTCGCTTTACCTTAGAATCGCACCCTTTATT
TGACGACACCTGCATAGTGCTTGGTCCAACACCATCGCCTCTCGCCAAACGCGCAGGCAAATACCGTTGGCAACTGTTAC
TCCAAACCCAGCACCGTTCATTAATGCAAAAGTTATTAACCAGTGCCAAACCTGCTATTGAGTTGTTGCCTAATGCCAAA
AAAGTTCGCTGGAATTTAGACATAGAACCACAAGATCTCAGCTAG

Domains

Predicted by InterProScan.

• DEAD/DEAH box helicase (DEAD) (220-365)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (446-472)

• Helicase conserved C-terminal domain (Helicase_C) (500-589)

• Primosomal protein N C-terminal domain (PriA_C) (633-730)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (7-102)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	43.148	100	0.433