Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   K8O95_RS00645 Genome accession   NZ_CP082887
Coordinates   131130..132569 (-) Length   479 a.a.
NCBI ID   WP_061476618.1    Uniprot ID   A0A175VGR9
Organism   Aeromonas enteropelogenes strain FDAARGOS_1459     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 126130..137569
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8O95_RS00615 (K8O95_00610) - 126470..127831 (+) 1362 WP_223379394.1 Do family serine endopeptidase -
  K8O95_RS00620 (K8O95_00615) degS 127922..129025 (+) 1104 WP_026456106.1 outer membrane-stress sensor serine endopeptidase DegS -
  K8O95_RS00625 (K8O95_00620) npr 129167..129439 (-) 273 WP_026456107.1 PTS phosphocarrier protein NPr -
  K8O95_RS00630 (K8O95_00625) rapZ 129460..130326 (-) 867 WP_042070881.1 RNase adapter RapZ -
  K8O95_RS00635 (K8O95_00630) ptsN 130372..130818 (-) 447 WP_005336322.1 PTS IIA-like nitrogen regulatory protein PtsN -
  K8O95_RS00640 (K8O95_00635) hpf 130821..131108 (-) 288 WP_005336323.1 ribosome hibernation promoting factor -
  K8O95_RS00645 (K8O95_00640) rpoN/rpoN1 131130..132569 (-) 1440 WP_061476618.1 RNA polymerase factor sigma-54 Machinery gene
  K8O95_RS00650 (K8O95_00645) lptB 132635..133360 (-) 726 WP_026456111.1 LPS export ABC transporter ATP-binding protein -
  K8O95_RS00655 (K8O95_00650) lptA 133364..133891 (-) 528 WP_026456112.1 lipopolysaccharide transport periplasmic protein LptA -
  K8O95_RS00660 (K8O95_00655) lptC 133878..134438 (-) 561 WP_026456113.1 LPS export ABC transporter periplasmic protein LptC -
  K8O95_RS00665 (K8O95_00660) kdsC 134435..134989 (-) 555 WP_124239993.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  K8O95_RS00670 (K8O95_00665) - 134989..135984 (-) 996 WP_201980594.1 KpsF/GutQ family sugar-phosphate isomerase -
  K8O95_RS00675 (K8O95_00670) mlaF 136192..136992 (+) 801 WP_026456358.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 54062.87 Da        Isoelectric Point: 4.3076

>NTDB_id=603357 K8O95_RS00645 WP_061476618.1 131130..132569(-) (rpoN/rpoN1) [Aeromonas enteropelogenes strain FDAARGOS_1459]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSSLELQQEIQQALENNPLLEQEESEVQETSPQDQVDASQLDSSDAMAQDQMP
DELPVDTTWDEVYSAGTAQSAGPIHDGDDTVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVSLED
IQAAVSSDEVEVELDEIEVVLKRVQHFDPVGIAARSVQECLLIQLEQYDPETPWIDEAKEVIQEHMELLGNRDYRTLARK
TRLKESDLKEVLDLIQLLEPRPGNGIIQSDSQYVIPDVSVVKRQNRWVVELNPDSSPRIRVNETYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLFIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=603357 K8O95_RS00645 WP_061476618.1 131130..132569(-) (rpoN/rpoN1) [Aeromonas enteropelogenes strain FDAARGOS_1459]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAATCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCTCTCTGGAACTTCAGCAGGAAATTCAGCAAGCGCTCGAAAACAATCCCCTGCTGGAGCAGGAAGAGAGCGAGG
TGCAGGAGACATCCCCGCAAGATCAGGTCGATGCCAGCCAGCTGGATTCCAGCGATGCCATGGCCCAGGATCAGATGCCG
GACGAGTTGCCGGTGGATACGACCTGGGACGAGGTCTATTCCGCGGGGACGGCCCAGAGTGCCGGACCCATCCATGATGG
GGATGACACCGTCTACCAGGGGGAGACCACCGAGAACCTGCAGGACTACCTGTTGTGGCAGATGCGCCTCACCCCGTTCA
GCGATGTGGATGCGGCCATCGCGCTTGCCATCATAGATGCCATCGACGAATCCGGTTATCTGACAGTGTCGCTGGAGGAT
ATCCAGGCGGCGGTGAGCAGCGATGAAGTGGAAGTCGAACTCGACGAAATCGAGGTCGTGCTCAAACGGGTGCAGCACTT
CGATCCCGTGGGGATCGCAGCTCGTTCGGTACAGGAGTGCCTGCTGATCCAGCTGGAACAGTACGACCCTGAGACCCCGT
GGATCGATGAGGCCAAGGAGGTGATCCAGGAGCATATGGAACTGCTTGGCAACCGCGATTACCGCACTCTGGCCCGCAAG
ACCCGGCTCAAGGAGAGCGACCTCAAGGAGGTGCTGGATCTGATCCAGCTGCTGGAGCCGCGGCCGGGCAACGGCATCAT
CCAGAGCGACTCCCAGTACGTGATCCCGGACGTCTCCGTAGTCAAACGGCAGAATCGCTGGGTGGTTGAACTCAATCCCG
ATTCATCCCCGCGCATTCGGGTCAACGAGACCTATGCCGCTATGGCACGTAATGCCCGTAGCAGCACGGATACCCAGTTT
ATCCGCTCCCACTTGCAGGAAGCCAAGTGGTTCATCAAGAGCCTTGAGAGCCGTAACGACACCCTGTTGAAAGTGGCCAG
CTGCATCGTCGAGCAGCAACAGGGTTTTTTCGAATATGGTGAAGAGGCGATGAAGCCGATGGTGCTCAACGATATCGCCG
AAGCGGTGGAGATGCACGAGTCGACCATCTCCCGTGTAACAACCCAGAAATACATGCATACCCCCCGCGGTATTTTCGAA
CTGAAGTTTTTTTTCTCCAGCCATGTGAATACAGAAGGAGGAGGAGAGTGCTCATCGACCGCAATCCGTGCGCTCATCAA
GAAGCTGGTGACGGCGGAGAACCCCGCCAAGCCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCTAGACGCACGATCGCTAAGTACCGCGAATCCCTGTTTATCCCACCTTCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A175VGR9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.184

100

0.493