Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   K8O84_RS10820 Genome accession   NZ_CP082862
Coordinates   2226610..2228091 (-) Length   493 a.a.
NCBI ID   WP_061957215.1    Uniprot ID   -
Organism   Cupriavidus pauculus strain FDAARGOS_1472     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2221610..2233091
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8O84_RS10790 (K8O84_10750) - 2221983..2222636 (-) 654 WP_150980147.1 LON peptidase substrate-binding domain-containing protein -
  K8O84_RS10795 (K8O84_10755) rapZ 2222695..2223570 (-) 876 WP_061957217.1 RNase adapter RapZ -
  K8O84_RS10800 (K8O84_10760) - 2223690..2224022 (-) 333 WP_061957216.1 PsiF family protein -
  K8O84_RS10805 (K8O84_10765) hprK 2224235..2225206 (-) 972 WP_008650955.1 HPr(Ser) kinase/phosphatase -
  K8O84_RS10810 (K8O84_10770) ptsN 2225268..2225723 (-) 456 WP_061957260.1 PTS IIA-like nitrogen regulatory protein PtsN -
  K8O84_RS10815 (K8O84_10775) hpf 2226206..2226559 (-) 354 WP_019449655.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  K8O84_RS10820 (K8O84_10780) rpoN/rpoN1 2226610..2228091 (-) 1482 WP_061957215.1 RNA polymerase factor sigma-54 Machinery gene
  K8O84_RS10825 (K8O84_10785) lptB 2228224..2229024 (-) 801 WP_150980146.1 LPS export ABC transporter ATP-binding protein -
  K8O84_RS10830 (K8O84_10790) lptA 2229044..2229691 (-) 648 WP_150980145.1 lipopolysaccharide transport periplasmic protein LptA -
  K8O84_RS10835 (K8O84_10795) lptC 2229757..2230383 (-) 627 WP_061957212.1 LPS export ABC transporter periplasmic protein LptC -
  K8O84_RS10840 (K8O84_10800) - 2230387..2231370 (-) 984 WP_061957211.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54976.33 Da        Isoelectric Point: 5.0432

>NTDB_id=603227 K8O84_RS10820 WP_061957215.1 2226610..2228091(-) (rpoN/rpoN1) [Cupriavidus pauculus strain FDAARGOS_1472]
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRVSADGSVNLQSAPAPAPSEPK
ANGDDRQDSQASGEDDSYGDSGGEDYGSDWSLDDFARRPQGDDDEKTPMQLREADPTLREFLMEQLTPLKISVRDKGLAI
FLIESLDDDGYLSASLEEICGELPEELEFEVDEVHAMLTLLQSFDPPGVGARNAAECLSLQLRRLKHPQRELALSIVNHH
LELLAVRDYTRLKKALQVDEIALKAAHELIRSLAPYPGHAYSRPEADFVVPDVFVRKSGNGWIAHLNPDVMPRLRINDMY
AQILRGAKGEAGAAGLQQKLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFSHGEIAMRPLVLREIADTLGLHESTISR
VTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDPRNPLSDSRIAELLGEQGFVVARRTVAKYRE
ALKIPAVNLRKSL

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=603227 K8O84_RS10820 WP_061957215.1 2226610..2228091(-) (rpoN/rpoN1) [Cupriavidus pauculus strain FDAARGOS_1472]
ATGAAACCTTCGCTTCAGCTTCGCCTTTCACAGCATCTTGCCCTGACACCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GCTTTCCACGCTGGAACTCCAGCAAGAGGTCGAGCAGGCCCTCACGGAAAACCCCCTGCTGGAACGCGAAAACGACTGGA
TCGAAAGCCCCTTGCGGGTGTCGGCCGATGGCTCCGTGAACCTGCAGAGCGCACCCGCGCCGGCTCCCTCGGAGCCCAAG
GCCAATGGCGACGATCGGCAGGACAGCCAGGCAAGTGGTGAGGACGACAGTTACGGCGATTCCGGCGGCGAAGACTACGG
CAGCGACTGGAGCCTCGACGATTTCGCGCGACGTCCGCAGGGTGACGATGACGAAAAAACGCCGATGCAATTGCGCGAGG
CCGATCCCACGCTGCGCGAATTCCTGATGGAGCAGCTGACGCCGCTGAAAATCTCCGTGCGCGACAAGGGCCTGGCTATC
TTCCTGATCGAATCGCTCGACGACGACGGCTACCTGAGCGCTTCGCTCGAGGAAATCTGCGGGGAACTCCCCGAGGAACT
CGAATTCGAAGTCGATGAAGTGCACGCCATGCTGACGTTGCTGCAGAGCTTTGATCCTCCCGGCGTGGGCGCGCGCAACG
CGGCTGAGTGTCTGAGTCTGCAGTTGCGGCGTTTGAAGCATCCGCAGCGCGAGTTGGCGTTGTCTATCGTCAATCATCAC
CTGGAACTGCTGGCCGTGCGCGACTACACGCGGCTCAAGAAAGCGCTCCAGGTTGACGAAATTGCATTAAAGGCCGCGCA
CGAGTTGATCCGGTCGCTTGCGCCGTATCCAGGGCATGCTTATAGCCGCCCGGAAGCGGATTTTGTGGTGCCGGACGTAT
TTGTGCGCAAAAGTGGAAACGGGTGGATTGCGCACCTGAATCCCGATGTCATGCCGCGTCTGCGCATCAACGACATGTAC
GCGCAAATCTTGCGCGGTGCAAAAGGCGAAGCGGGTGCGGCGGGACTGCAGCAGAAGCTCCAGGAGGCGCGGTGGCTCAT
CAAGAACATCCAGCAGCGTTTCGATACGATCCTGCGTGTGGCGCAGGCAATCGTAGAGCGTCAAAAGAACTTTTTCTCGC
ACGGGGAAATCGCAATGCGCCCCTTGGTTTTGCGGGAAATTGCCGATACACTCGGTTTACATGAGTCGACCATTTCCCGG
GTGACTACGAACAAATACATGGCCACACCAATGGGTACTTTCGAGTTGAAGTACTTCTTTGGCAGCCACGTCTCCACCGA
GACGGGCGGTGCGGCCTCCTCCACGGCGATCCGCGCGCTGATCAAGCAACTGATAGGAGCCGAAGACCCCAGGAATCCCC
TTTCCGACAGCCGCATCGCCGAATTGCTGGGCGAGCAGGGTTTCGTGGTTGCACGCCGCACTGTGGCCAAATACCGCGAA
GCGCTGAAGATCCCCGCAGTCAATCTCCGCAAGTCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(130-320)

Sigma-54, DNA binding domain (Sigma54_DBD)

(336-491)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

79.88

100

0.813