Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   K8P07_RS02830 Genome accession   NZ_CP082860
Coordinates   585741..587174 (-) Length   477 a.a.
NCBI ID   WP_014171817.1    Uniprot ID   A0A839BTX8
Organism   Enterobacter ludwigii strain FDAARGOS_1475     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 580741..592174
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8P07_RS02800 (K8P07_02795) elbB 582274..582927 (+) 654 WP_032670144.1 isoprenoid biosynthesis glyoxalase ElbB -
  K8P07_RS02805 (K8P07_02800) mtgA 582924..583649 (+) 726 WP_073396304.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  K8P07_RS02810 (K8P07_02805) npr 583688..583960 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  K8P07_RS02815 (K8P07_02810) rapZ 583957..584811 (-) 855 WP_014171820.1 RNase adapter RapZ -
  K8P07_RS02820 (K8P07_02815) ptsN 584857..585348 (-) 492 WP_014171819.1 PTS IIA-like nitrogen regulatory protein PtsN -
  K8P07_RS02825 (K8P07_02820) hpf 585431..585718 (-) 288 WP_014171818.1 ribosome hibernation promoting factor -
  K8P07_RS02830 (K8P07_02825) rpoN/rpoN1 585741..587174 (-) 1434 WP_014171817.1 RNA polymerase factor sigma-54 Machinery gene
  K8P07_RS02835 (K8P07_02830) lptB 587222..587947 (-) 726 WP_014171816.1 LPS export ABC transporter ATP-binding protein -
  K8P07_RS02840 (K8P07_02835) lptA 587954..588508 (-) 555 WP_020883526.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  K8P07_RS02845 (K8P07_02840) lptC 588477..589052 (-) 576 WP_014171814.1 LPS export ABC transporter periplasmic protein LptC -
  K8P07_RS02850 (K8P07_02845) kdsC 589049..589615 (-) 567 WP_014171813.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  K8P07_RS02855 (K8P07_02850) kdsD 589630..590616 (-) 987 WP_020883527.1 arabinose-5-phosphate isomerase KdsD -
  K8P07_RS02860 (K8P07_02855) - 590630..591607 (-) 978 WP_223398702.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54206.00 Da        Isoelectric Point: 4.3224

>NTDB_id=603175 K8P07_RS02830 WP_014171817.1 585741..587174(-) (rpoN/rpoN1) [Enterobacter ludwigii strain FDAARGOS_1475]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQADLHEEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESMGDEEIELEEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSVRNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTILSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=603175 K8P07_RS02830 WP_014171817.1 585741..587174(-) (rpoN/rpoN1) [Enterobacter ludwigii strain FDAARGOS_1475]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCGATTCGCCTGTTGCA
ACTGTCCACCTTAGAACTCCAGCAGGAGCTCCAGCAAGCGCTGGATAGCAATCCGTTGCTGGAGCAAGCCGATCTTCATG
AAGAGGTAGATACCCAGCAGTCGCAGGATACTGAAGCACTCGACACCGCCGATGCGCTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGACGA
TGAGCTACCGGTTTATCAGGGTGAAACTACCCAGTCACTGCAGGATTACCTGATGTGGCAGGTGGAACTTACCCCCTTCT
CCGATACCGACCGCGCGATAGCGACATCCATTGTCGATGCCGTGGATGACACCGGTTATCTGACCGTCACGCTGGACGAA
ATTCTGGAAAGCATGGGTGACGAAGAGATTGAACTCGAGGAGATAGAAGCCGTTCTGAAGCGCATTCAGCGTTTCGATCC
GGTTGGGGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTTATCCAACTTTCTCAGTTCAGCAAAGAGACGCCATGGCTTG
ATGAAGCCCGCTTGATCATCAGCGACCATCTGGATCTTCTGGCCAATCACGATTTCCGCAGCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAAGAGGCGGTCAATCTGATTCAGTCCCTCGATCCGCGCCCCGGTCAGTCCATTCAGACCAG
CGAACCCGAGTACGTCATCCCGGATGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAACTCGGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGTGCGTAACGACTCCGATAATCAATACATTCGT
AGCAATTTGCAGGAAGCGCGATGGTTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTCCGCGTAAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGTGAAGAGTATATGAAACCGATGGTGTTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGTGAAGCGTCGTCAACGGCCATTCGCGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTGAGCGACAGTAAGTTAACCACCATACTGTCCGACCAGGGTATTATGGTGG
CACGCCGAACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A839BTX8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.941

100

0.486