Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   K8O86_RS08475 Genome accession   NZ_CP082850
Coordinates   1912724..1913287 (+) Length   187 a.a.
NCBI ID   WP_003632473.1    Uniprot ID   D3HQQ0
Organism   Legionella longbeachae strain FDAARGOS_1481     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1907724..1918287
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8O86_RS08465 (K8O86_08475) - 1909988..1911418 (-) 1431 WP_080619813.1 M protein -
  K8O86_RS08470 (K8O86_08480) pilM 1911655..1912719 (+) 1065 WP_003632474.1 type IV pilus assembly protein PilM Machinery gene
  K8O86_RS08475 (K8O86_08485) pilN 1912724..1913287 (+) 564 WP_003632473.1 PilN domain-containing protein Machinery gene
  K8O86_RS08480 (K8O86_08490) pilO 1913284..1913883 (+) 600 WP_012978910.1 type 4a pilus biogenesis protein PilO Machinery gene
  K8O86_RS08485 (K8O86_08495) pilP 1913880..1914386 (+) 507 WP_003632471.1 pilus assembly protein PilP Machinery gene
  K8O86_RS08490 (K8O86_08500) pilQ 1914395..1916500 (+) 2106 WP_012978911.1 type IV pilus secretin PilQ Machinery gene
  K8O86_RS08495 (K8O86_08505) aroK 1916836..1917363 (+) 528 WP_003632469.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 187 a.a.        Molecular weight: 21920.72 Da        Isoelectric Point: 7.3178

>NTDB_id=603011 K8O86_RS08475 WP_003632473.1 1912724..1913287(+) (pilN) [Legionella longbeachae strain FDAARGOS_1481]
MTQINLLPWREQKREQEKKLFMVLFFIVIALSIFIVFLINTYASDLVSNQLVRNQMLQDEIAAMNSQLIEIKNLKKTREL
FISRMFIVQHLQSTRTLMVHLFDELINVMPSGIYLTKVEGKNDILSVTGYTESNTYVSILMKNIEINEWIHSPVLSEIKK
EDRQEPANNEFKLNFVLAPQFQLGIIR

Nucleotide


Download         Length: 564 bp        

>NTDB_id=603011 K8O86_RS08475 WP_003632473.1 1912724..1913287(+) (pilN) [Legionella longbeachae strain FDAARGOS_1481]
ATGACACAAATTAATCTTCTTCCTTGGCGAGAACAAAAGCGGGAGCAAGAAAAAAAGCTGTTCATGGTGTTATTTTTCAT
AGTTATAGCGCTTTCAATATTTATTGTGTTTTTAATTAATACTTATGCCTCTGATTTAGTAAGTAATCAACTTGTTCGAA
ATCAGATGCTGCAAGATGAAATAGCGGCTATGAATAGCCAGTTAATTGAAATTAAAAATTTGAAAAAAACTCGAGAATTA
TTCATTTCCAGAATGTTTATAGTTCAGCATTTGCAATCAACCAGAACCTTGATGGTTCATTTATTTGATGAATTAATTAA
TGTAATGCCTTCCGGTATTTATTTAACAAAGGTAGAAGGAAAAAATGATATTCTTTCAGTCACTGGTTATACTGAATCAA
ATACCTATGTTTCAATTTTAATGAAAAATATAGAAATTAATGAATGGATCCATTCTCCTGTTCTGAGCGAAATTAAGAAG
GAAGACCGTCAGGAACCTGCTAATAATGAATTTAAGTTAAATTTTGTTCTTGCCCCTCAATTTCAGTTGGGGATTATACG
ATGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-175)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D3HQQ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

62.295

97.861

0.61