Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   K8B66_RS15645 Genome accession   NZ_CP082807
Coordinates   3380252..3381757 (+) Length   501 a.a.
NCBI ID   WP_039346023.1    Uniprot ID   A0A1E3FHP0
Organism   Burkholderia contaminans strain SCAID TST1-2021 (11/270)     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3375252..3386757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B66_RS15620 (K8B66_15620) kdsD 3376496..3377479 (+) 984 WP_039345999.1 arabinose 5-phosphate isomerase KdsD -
  K8B66_RS15625 (K8B66_15625) - 3377479..3378015 (+) 537 WP_039346001.1 HAD family hydrolase -
  K8B66_RS15630 (K8B66_15630) lptC 3378019..3378633 (+) 615 WP_039346004.1 LPS export ABC transporter periplasmic protein LptC -
  K8B66_RS15635 (K8B66_15635) lptA 3378654..3379319 (+) 666 WP_039346006.1 lipopolysaccharide transport periplasmic protein LptA -
  K8B66_RS15640 (K8B66_15640) lptB 3379316..3380092 (+) 777 WP_039346017.1 LPS export ABC transporter ATP-binding protein -
  K8B66_RS15645 (K8B66_15645) rpoN/rpoN1 3380252..3381757 (+) 1506 WP_039346023.1 RNA polymerase factor sigma-54 Machinery gene
  K8B66_RS15650 (K8B66_15650) hpf 3381916..3382275 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  K8B66_RS15655 (K8B66_15655) - 3382444..3382983 (+) 540 WP_011353226.1 PTS sugar transporter subunit IIA -
  K8B66_RS15660 (K8B66_15660) hprK 3383113..3384081 (+) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  K8B66_RS15665 (K8B66_15665) rapZ 3384157..3385065 (+) 909 WP_039346027.1 RNase adapter RapZ -
  K8B66_RS15670 (K8B66_15670) - 3385093..3385728 (+) 636 WP_039346030.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54755.86 Da        Isoelectric Point: 4.8740

>NTDB_id=602427 K8B66_RS15645 WP_039346023.1 3380252..3381757(+) (rpoN/rpoN1) [Burkholderia contaminans strain SCAID TST1-2021 (11/270)]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPTA
TNGSSSSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=602427 K8B66_RS15645 WP_039346023.1 3380252..3381757(+) (rpoN/rpoN1) [Burkholderia contaminans strain SCAID TST1-2021 (11/270)]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACACCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGTGTCGCCGCGGACGGATCGCTGATCGCGCAGACGCCTCCCGCGCAGAACACCGAGCCCACTGCG
ACAAACGGTAGCAGTTCGTCGAGCGATCGTGCCGAGCGCGACGAGCCGGCCGGCGCGGACGAATACGACGGTTACGGGTC
CGGCGACGGCAACGACGGCCCGCAGTGGAACCTCGACGAATTCGGCCGCTCGAGCGGCGCATCCGACGACGACGACCTCC
CGCCGCTGCAGGTACAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGATGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAGATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGATCCGG
CCGGGGTCGGTGCCCGTTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTCGATCCGTCCCCGACACGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCCCGCGACTTCACGCGGTTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGTTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTTTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGTTCGCTCAAGCAGCA
GCTCCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGTTTCGACACGATCCTGCGTGTCGCGCAGGCGATTGTCG
AGCGTCAAAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTTTCGACCGACACCGGTGGCGCCGCTTCGTCGACAGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCTAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAAGGATTCGTGGTCGCGCGC
CGCACGGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E3FHP0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.379

100

0.691