Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   K6959_RS10900 Genome accession   NZ_CP082780
Coordinates   2254233..2255267 (-) Length   344 a.a.
NCBI ID   WP_163239327.1    Uniprot ID   A0A6B3VWM2
Organism   Bacillus aquiflavi strain 3H-10     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2249233..2260267
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6959_RS10860 (K6959_10860) - 2250584..2250772 (-) 189 WP_163239302.1 YqzE family protein -
  K6959_RS10865 (K6959_10865) - 2250784..2251320 (-) 537 WP_163239305.1 shikimate kinase -
  K6959_RS10870 (K6959_10870) comGG 2251335..2251715 (-) 381 WP_163239309.1 competence type IV pilus minor pilin ComGG -
  K6959_RS10875 (K6959_10875) comGF 2251712..2252179 (-) 468 WP_163239312.1 competence type IV pilus minor pilin ComGF -
  K6959_RS10880 (K6959_10880) - 2252133..2252462 (-) 330 WP_163239315.1 hypothetical protein -
  K6959_RS10885 (K6959_10885) comGD 2252446..2252898 (-) 453 WP_223086495.1 competence type IV pilus minor pilin ComGD -
  K6959_RS10890 (K6959_10890) comGC 2252891..2253202 (-) 312 WP_163239321.1 competence type IV pilus major pilin ComGC -
  K6959_RS10895 (K6959_10895) comGB 2253215..2254246 (-) 1032 WP_223086497.1 competence type IV pilus assembly protein ComGB -
  K6959_RS10900 (K6959_10900) comGA 2254233..2255267 (-) 1035 WP_163239327.1 competence type IV pilus ATPase ComGA Machinery gene
  K6959_RS10910 (K6959_10910) - 2255705..2256406 (+) 702 WP_223086499.1 metalloregulator ArsR/SmtB family transcription factor -
  K6959_RS10915 (K6959_10915) - 2256481..2256708 (+) 228 WP_163239333.1 DUF2626 domain-containing protein -
  K6959_RS10920 (K6959_10920) - 2256761..2257855 (-) 1095 WP_163239336.1 class I SAM-dependent methyltransferase -
  K6959_RS10925 (K6959_10925) - 2258031..2258663 (-) 633 WP_223086501.1 MBL fold metallo-hydrolase -
  K6959_RS10930 (K6959_10930) - 2258781..2259287 (+) 507 WP_223086503.1 hypothetical protein -
  K6959_RS10935 (K6959_10935) - 2259349..2259519 (+) 171 WP_163239342.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39372.63 Da        Isoelectric Point: 9.0286

>NTDB_id=602145 K6959_RS10900 WP_163239327.1 2254233..2255267(-) (comGA) [Bacillus aquiflavi strain 3H-10]
MNWIEQLADKIITDAFKKETTDIHIIPRKKDTLIQFRVVNKLIPYLYLQREECDRLISHFKFQAAMDIGERRRPQSGAYT
NYVDGHLISMRLSTLPTAYNESLVIRLLPQSKKDPHHLSLFPQTTRKILALLKHAHGLIIFTGPTGSGKTTTLYSLLNES
SRIFNRNIITLEDPVEKNYDNVLQVQVNEKAGITYSTGLKAILRHDPDLIMVGEIRDAETAQIAVRASLTGHLVLSTMHT
RDAKGAIYRLLEFGVNWIEIEQTLVAVTAQRLVELACPLSETVKRAAVFEILYGRSLVEVLREAKGEKVDLHYEKLKDII
KKGIALGFIKESEYERWVYEYEKV

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=602145 K6959_RS10900 WP_163239327.1 2254233..2255267(-) (comGA) [Bacillus aquiflavi strain 3H-10]
TTGAACTGGATTGAACAATTAGCTGATAAAATTATAACGGATGCCTTCAAAAAGGAAACGACAGACATTCATATTATTCC
GCGAAAAAAAGACACGCTGATTCAATTTAGAGTAGTAAACAAATTAATTCCGTATCTTTATTTACAGCGAGAGGAATGTG
ATCGGCTCATTTCTCACTTTAAATTCCAAGCAGCAATGGACATTGGAGAACGAAGGCGGCCGCAAAGTGGCGCTTATACA
AATTATGTTGATGGTCATTTAATTAGCATGCGTCTTTCAACGCTTCCTACAGCTTATAATGAAAGCCTCGTCATTCGCCT
ATTGCCCCAGTCTAAAAAGGATCCACATCATCTTTCTTTGTTTCCTCAAACAACTCGGAAAATCCTAGCTCTTTTAAAAC
ATGCCCACGGTTTAATAATTTTTACAGGTCCAACAGGTAGCGGCAAAACGACAACACTTTATTCACTTCTGAATGAATCC
TCTCGTATTTTCAATCGCAATATCATTACACTAGAAGATCCAGTTGAAAAAAATTATGACAATGTTTTACAAGTTCAAGT
AAATGAGAAGGCTGGAATTACGTATTCCACAGGTTTAAAAGCAATTTTACGGCACGATCCTGATCTGATTATGGTTGGTG
AGATCCGTGATGCAGAAACGGCGCAAATTGCCGTACGAGCTTCACTTACAGGTCATTTAGTTCTCTCTACAATGCACACG
CGAGATGCAAAAGGGGCAATTTATCGATTACTTGAATTTGGTGTTAATTGGATTGAAATCGAACAAACTTTAGTAGCGGT
GACAGCACAACGATTAGTAGAGCTAGCTTGTCCGCTGAGCGAAACGGTGAAAAGAGCAGCTGTATTCGAGATTTTGTATG
GGAGGTCTCTAGTTGAAGTTTTACGGGAAGCGAAAGGGGAGAAGGTTGATCTTCATTATGAGAAGTTAAAGGATATTATT
AAAAAAGGAATCGCGCTCGGATTTATTAAAGAGTCAGAGTATGAACGTTGGGTGTACGAATATGAGAAGGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6B3VWM2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.102

100

0.584
  pilB Glaesserella parasuis strain SC1401

40.401

100

0.41
  pilB Haemophilus influenzae 86-028NP

37.059

98.837

0.366
  pilB Vibrio cholerae strain A1552

38.994

92.442

0.36