Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   K6959_RS03760 Genome accession   NZ_CP082780
Coordinates   740901..744416 (+) Length   1171 a.a.
NCBI ID   WP_223087670.1    Uniprot ID   -
Organism   Bacillus aquiflavi strain 3H-10     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 735901..749416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6959_RS03750 (K6959_03750) ltrA 737223..738506 (+) 1284 WP_223087669.1 group II intron reverse transcriptase/maturase -
  K6959_RS03755 (K6959_03755) - 738843..740501 (+) 1659 WP_163243487.1 DASS family sodium-coupled anion symporter -
  K6959_RS03760 (K6959_03760) addB 740901..744416 (+) 3516 WP_223087670.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  K6959_RS03765 (K6959_03765) addA 744385..745656 (+) 1272 WP_163243489.1 UvrD-helicase domain-containing protein Machinery gene
  K6959_RS03770 (K6959_03770) addA 746347..748833 (+) 2487 Protein_741 helicase-exonuclease AddAB subunit AddA -
  K6959_RS03775 (K6959_03775) - 748974..749192 (-) 219 WP_163243505.1 spore germination protein -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 135711.17 Da        Isoelectric Point: 7.9327

>NTDB_id=602131 K6959_RS03760 WP_223087670.1 740901..744416(+) (addB) [Bacillus aquiflavi strain 3H-10]
MSVRFIIGRSGTGKTANCLNEIRTSLLRSPDGSPIIYLVPDQMTFLSEYNLMTTPHLGGMIRAQVYSFSRLAWRILQDTG
GGSRYHLNNIGVNMLIRKIIEEKKDELKLFQLAADKNGFIEQMEQILTEFKQYCIRPEELTDQHRLLSEENKGLQDKLHD
LELIYRYFEQALFGKYVDSEDYFRLMAEKIAQSDYLKNAEIFIDGFHSFTPQEYMVITQLMKHCQRVSIALTVDQPYIHF
GPDELSLFRMTGETFQTLYNIAQSEGIKIEKNNLLYEQKRWRDPSLIHLESYFDSRPAIPFQKKARIHLYEAANRRAEIE
GVARKINKLIRSKGYRYRDIAILMRNGNDYFDIIETIFNDYGIPIFIDQKRTMLNHPLIELIRSTLDIINSNWSYESVFR
AVKTELLFPKNENNQQIREKFDRLENYVLAYGIQGEKWTKRDRWIYRRFYGIEFENNVQTDQEKLVELELNELRLLITAP
ILRLARRLKRAKNGRLLSEAIFLYLEELDIPEKLEKWKLEAEQAGKLVKSREHDQAWNGMIELLDQFVELFGNEKLSLKQ
FSVIFEAGIESLRFSLVPPAIDQVLVADLEKSRLSDVKAAFIIGLNEGVLPAKMIDEGIVADDDREILQQKGLTIAPTSK
TKLLDESFIAYKAFTTPSDELYLTYPIANEEGKALLPSFYIKRINDLFPAVEKGLFAIDPAPLPELDQLEYAANENMTLS
YLTAQLQLKKRNYPIYDFWWDVYNYYLKSNSWSQLAKKVLASLFYENKSKQKQLSSETTRQLYGETIEASVSRMELFHSC
PFSHFVKHGLKLRKRQVYRLEAPDIGELFHAALKYIADTIMKQNVSWSSLTKQQCRALARQAIDNIAPKLQNEILLSSNR
HHYIKRKLENVLSRTSLVLSEHAKASGFSPVGVEVGFGPKGPLPPLEFRLKNDTKMNLVGRIDRIDKAEAQTGVFLRIVD
YKSSIKDLNLTEVYYGLALQMLTYLDIIVTHANTLIGMDAMPAGVLYFHVHNPMIQAKKMLTIDELEQEIYKQFKMKGLM
LADQNVIRLMDKSLDSGESKIVSAGIKKDGTLSKRSKVANSEQLNHLRHYVRNSYLKTGNEIMAGKIDISPYKLKNKTPC
TFCSFKTVCHFDQTIEQNDYRILVPQKDEDIIEFIRREVAENERNEHTAEA

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=602131 K6959_RS03760 WP_223087670.1 740901..744416(+) (addB) [Bacillus aquiflavi strain 3H-10]
ATGTCTGTCCGTTTTATCATCGGACGTTCAGGGACAGGAAAGACCGCAAACTGTTTAAACGAAATAAGAACAAGCTTATT
GAGAAGTCCTGACGGATCACCAATCATATACTTAGTTCCAGATCAAATGACATTTTTATCAGAATACAACTTAATGACAA
CTCCTCATCTTGGTGGAATGATTAGAGCTCAAGTGTATAGCTTTTCACGGCTTGCTTGGCGAATTTTGCAAGATACTGGC
GGAGGAAGCAGATATCATTTAAATAATATCGGTGTCAATATGTTAATTAGGAAAATAATTGAGGAAAAAAAGGATGAGCT
CAAACTTTTTCAATTAGCTGCTGATAAAAACGGATTCATTGAACAGATGGAACAAATATTAACTGAGTTTAAGCAGTATT
GTATTCGTCCGGAAGAATTAACAGATCAGCATAGATTACTTTCTGAAGAAAATAAAGGCTTACAAGATAAACTTCATGAT
CTTGAATTAATATATCGTTATTTTGAACAAGCTCTTTTTGGAAAATATGTTGATTCTGAAGATTATTTCCGTCTTATGGC
AGAAAAAATAGCTCAATCTGACTATTTAAAAAATGCTGAAATATTTATTGATGGATTTCATAGTTTTACTCCTCAAGAAT
ATATGGTGATTACCCAATTAATGAAACATTGTCAGCGAGTATCGATTGCATTAACTGTCGATCAGCCTTACATTCATTTT
GGGCCAGATGAGCTAAGTTTATTTCGGATGACTGGGGAAACCTTCCAAACACTATATAATATCGCTCAATCAGAAGGAAT
TAAAATAGAAAAGAACAATCTGTTATATGAACAAAAGCGCTGGCGTGATCCTTCCCTCATCCATTTAGAATCATACTTTG
ATTCTCGTCCAGCAATTCCGTTTCAAAAAAAAGCAAGAATCCATTTATATGAAGCTGCTAATCGCAGGGCAGAAATTGAA
GGGGTTGCTAGAAAAATTAATAAACTCATACGTTCTAAAGGGTATCGTTATCGCGATATTGCAATCTTAATGAGGAACGG
GAACGATTATTTTGACATAATTGAAACGATTTTTAATGATTATGGAATTCCGATTTTTATTGACCAAAAAAGAACGATGC
TAAATCATCCGTTAATAGAACTTATCCGGTCTACACTAGATATCATTAATAGTAATTGGAGTTATGAATCAGTATTTCGC
GCAGTTAAAACTGAGTTGCTTTTTCCAAAAAATGAAAACAATCAGCAAATTCGTGAAAAGTTTGATAGACTTGAAAACTA
TGTATTAGCATACGGAATTCAAGGAGAAAAGTGGACGAAGCGAGATCGTTGGATTTATCGTCGATTTTACGGAATAGAAT
TTGAAAATAATGTTCAAACCGATCAAGAGAAACTAGTTGAATTAGAGTTAAACGAGCTCAGACTGCTTATCACTGCTCCT
ATTTTGCGATTAGCGCGCAGGCTAAAAAGAGCAAAGAACGGACGATTGCTTTCTGAAGCGATTTTTTTATATCTCGAAGA
ACTTGATATACCTGAGAAATTAGAAAAGTGGAAATTAGAGGCTGAACAAGCAGGTAAATTAGTAAAAAGTAGAGAGCATG
ACCAAGCGTGGAATGGGATGATTGAACTTCTTGATCAATTTGTAGAGTTATTCGGAAACGAGAAGTTATCTTTAAAGCAA
TTTTCAGTTATTTTCGAAGCAGGGATTGAATCTTTGCGATTTTCTCTTGTACCGCCAGCAATCGATCAAGTGCTCGTTGC
TGATCTTGAAAAATCGCGTTTGTCTGACGTAAAAGCAGCATTTATTATTGGACTAAACGAAGGAGTTCTCCCTGCAAAAA
TGATTGACGAGGGAATTGTAGCGGATGATGATCGGGAAATATTACAGCAAAAAGGTTTGACAATTGCTCCAACTAGTAAA
ACAAAATTACTTGATGAGTCATTTATTGCTTATAAAGCATTTACAACTCCTTCAGACGAGCTTTATTTAACATATCCGAT
CGCAAATGAAGAAGGAAAAGCATTGCTGCCTTCATTTTATATAAAAAGAATAAACGATTTGTTTCCGGCGGTGGAAAAAG
GTCTTTTTGCAATTGATCCTGCGCCATTACCAGAATTGGATCAGCTTGAATATGCTGCAAACGAAAACATGACACTGTCG
TATTTAACTGCCCAATTACAATTGAAAAAAAGAAATTATCCGATCTATGATTTTTGGTGGGATGTATACAACTATTACTT
AAAAAGTAATAGTTGGTCACAGTTAGCTAAAAAAGTATTAGCAAGTTTATTTTATGAAAATAAATCGAAACAGAAACAGC
TTTCAAGCGAAACAACTCGTCAATTATATGGAGAAACGATTGAAGCCAGTGTATCAAGAATGGAGCTATTTCATAGCTGT
CCTTTTTCTCATTTTGTTAAGCACGGACTAAAATTGCGGAAGCGACAAGTTTATCGTTTAGAAGCACCTGATATTGGAGA
GTTATTTCATGCCGCATTAAAATATATTGCAGATACAATAATGAAACAAAATGTTAGTTGGTCAAGCTTAACAAAACAAC
AGTGTCGGGCCTTGGCAAGACAAGCAATTGACAACATAGCGCCAAAATTACAAAATGAAATCTTACTTAGTTCCAATAGA
CACCATTACATAAAGCGAAAGCTTGAAAATGTGCTTAGCCGCACGTCTCTAGTCTTAAGTGAACACGCGAAAGCTAGCGG
TTTTTCTCCGGTTGGTGTTGAAGTAGGATTCGGACCAAAAGGACCGTTGCCTCCACTTGAATTTCGCTTAAAAAATGATA
CAAAAATGAATTTAGTCGGTAGAATCGATAGAATTGATAAAGCCGAAGCTCAGACAGGTGTCTTTTTAAGAATAGTTGAT
TATAAATCAAGTATAAAAGATTTAAATTTAACTGAAGTTTATTACGGTCTTGCATTACAAATGCTCACTTACTTAGACAT
TATTGTTACCCATGCAAACACATTAATCGGAATGGATGCAATGCCAGCAGGGGTGTTGTACTTTCATGTTCATAATCCAA
TGATTCAAGCGAAAAAGATGCTGACAATTGATGAGCTTGAACAAGAAATTTATAAACAGTTTAAAATGAAAGGATTGATG
CTAGCTGATCAAAATGTCATTCGTCTTATGGATAAAAGCCTTGATAGCGGCGAGTCGAAAATAGTTTCAGCAGGGATAAA
AAAAGACGGGACATTATCAAAACGATCAAAAGTTGCTAATAGTGAACAGTTGAATCACTTAAGGCATTATGTTCGAAATA
GTTATCTAAAAACAGGAAATGAGATTATGGCAGGAAAAATAGACATTTCCCCTTATAAACTGAAAAATAAAACTCCGTGT
ACTTTCTGTTCATTTAAAACAGTTTGTCACTTTGATCAAACGATTGAACAGAACGATTATCGGATATTAGTCCCGCAAAA
AGATGAAGATATCATAGAGTTTATTAGAAGGGAGGTTGCTGAAAATGAACGAAATGAACATACCGCCGAAGCCTAG

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(789-1130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

50.852

100

0.51