Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   K7T73_RS03955 Genome accession   NZ_CP082363
Coordinates   762177..765881 (+) Length   1234 a.a.
NCBI ID   WP_223662842.1    Uniprot ID   -
Organism   Bacillus badius strain NBPM-293     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 757177..770881
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K7T73_RS03940 (K7T73_03935) - 757494..757727 (-) 234 WP_041100226.1 IDEAL domain-containing protein -
  K7T73_RS03945 (K7T73_03940) - 757938..758555 (+) 618 WP_041099171.1 TVP38/TMEM64 family protein -
  K7T73_RS03950 (K7T73_03945) addB 758694..762173 (+) 3480 WP_223662840.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  K7T73_RS03955 (K7T73_03950) addA 762177..765881 (+) 3705 WP_223662842.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  K7T73_RS03960 (K7T73_03955) - 766077..767270 (+) 1194 WP_063383911.1 DUF418 domain-containing protein -
  K7T73_RS03965 (K7T73_03960) - 767275..768177 (+) 903 WP_223662844.1 fumarylacetoacetate hydrolase family protein -
  K7T73_RS03970 (K7T73_03965) - 768253..768615 (+) 363 WP_041099181.1 YisL family protein -
  K7T73_RS03975 (K7T73_03970) - 768732..768914 (+) 183 WP_223662846.1 hypothetical protein -
  K7T73_RS03980 (K7T73_03975) - 769219..770727 (+) 1509 WP_223662848.1 alpha-amylase family glycosyl hydrolase -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 140913.52 Da        Isoelectric Point: 4.9802

>NTDB_id=601808 K7T73_RS03955 WP_223662842.1 762177..765881(+) (addA) [Bacillus badius strain NBPM-293]
MKELIIPDKPNDVSWTDDQWKAITAEGRDILVAAAAGSGKTAVLVERIIRKITDKEAPINIDELLIVTFTNAAAAEMRHR
IGTALEAEMEKDPSSNHLRRQLTLLNKAPISTLHSFCLEVIRKYYYLIDVDPAFRIADEGELALLREEVLDDFMEEEYGR
EGNEGFYQLVDTFSSDRSDEELQKMIQTLYDFAQSHPRPEEWLEGLSQMYDESGSGGIDQLPFMESLKFYIGLQLEAAEE
MLAECLQMAQMPGGPAPREEMFTVERQTILRLQEALPSWEKLYEEMQKVEFKTLKSYKGEAYDEELKEQSKQLRTEAKKI
VEKLKEEYFSHQPASYISSMKEMKELIAALARLVGRFAGKFQAVKREKGIVDFSDLEHLALAILTGEEGGPSEAAQAYQE
QFKEIFLDEYQDFNIVQESIIQLVKTGDEENGNVFMVGDVKQSIYRFRQAEPNLFLSKYRRFSTDGQSSGLRIDLSQNFR
SRAEVLAGTNFLFKQIMDVKIGEIEYDRQAELVKGAAYPEEKPFPVEVTIIDQEGAGQSEDPDMKDAAQSELEARWIAGK
IKDLVESRHPVYDPKTKSERPVEYRDIVILLRSMTWAGVIKEEFKRAGIPLHAKVAGGYFQTMEIAVMLSLLKVIDNPYQ
DIPLASVLRSPIMRCTENDLAVIRLASKSGSYYEAAKAFAASRPEVRYEELHEKVSKFLEQLAEWRSLSRYQALSALVWQ
LYHDTHFYDFVGGMPGGKQRQANLRALYDRVRQYEETSFRGLFRFLRFVERMQERGEDLGEAGALSEQEDVVRLMTIHAS
KGLEFPVVFVAGLNRSFNMTDLNKSYLLDKELGIGTKYVNAEKRISYPSLPQLALKRKKRFELLAEEMRVLYVALTRAKE
KLFLLGSVKSAAKEAAKWERAARHPDWLLGSAARVSAKSYLDWVGPALVRHLDYTKAEQTRPAVFVDHPSRWSIQTVDAE
ALQPASLEQAEASGGLLERVQAFEPVPASSTFKEEITARMNWAYEYEAATHLRSKQSVSDLKRMNEIFNEGADNDLARHY
QRPVFRRPSFMQTKSMTPAEAGTALHTVMQHVPLQEAPDAAEVGQLLRTLVARELLTEEQAASVDPEKIAFFFRTDIGRE
LLQAEEIHRETPFNMAIDAGEIYPDWKGPEEAVLVQGVIDCLFEKDGKLYLLDYKTDQITGRFPGGMEQARPLLKKRYEV
QIQLYAQAVERIWKRKVDYKLLFFFDGGYLLPIE

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=601808 K7T73_RS03955 WP_223662842.1 762177..765881(+) (addA) [Bacillus badius strain NBPM-293]
ATGAAGGAGCTGATCATACCTGATAAACCGAACGATGTATCCTGGACGGATGACCAGTGGAAGGCGATCACCGCAGAAGG
GCGCGATATCCTCGTTGCGGCTGCTGCCGGCTCAGGAAAGACAGCGGTTCTTGTTGAACGGATTATTCGCAAAATTACCG
ATAAAGAAGCACCGATAAATATCGACGAGCTGCTAATTGTCACATTTACCAATGCGGCTGCTGCTGAAATGCGCCACCGT
ATCGGCACAGCACTAGAAGCAGAAATGGAGAAAGATCCGTCATCTAATCATTTGCGCCGGCAGTTGACCTTATTAAATAA
GGCGCCTATTTCTACTCTCCACTCTTTCTGTCTGGAAGTCATCCGCAAATATTATTATTTAATTGATGTGGACCCTGCTT
TTAGAATTGCTGATGAAGGGGAGCTGGCATTGCTGCGGGAAGAAGTACTCGATGATTTTATGGAAGAAGAGTATGGCAGA
GAAGGCAATGAAGGGTTTTACCAGCTTGTTGATACATTCTCAAGTGACCGCAGCGATGAAGAGCTGCAAAAAATGATCCA
AACGCTGTATGACTTTGCGCAGTCCCATCCGAGACCTGAGGAGTGGCTGGAGGGGCTTTCACAAATGTATGACGAAAGCG
GCAGCGGGGGAATCGATCAGCTGCCGTTTATGGAGTCTTTAAAGTTTTATATTGGGCTTCAGCTTGAAGCTGCTGAAGAA
ATGCTCGCGGAGTGCCTGCAGATGGCCCAGATGCCTGGCGGCCCAGCTCCCCGTGAAGAAATGTTTACTGTCGAGCGGCA
AACCATCTTGAGGCTTCAAGAAGCGCTTCCTTCTTGGGAGAAGCTCTATGAAGAAATGCAAAAAGTAGAGTTTAAGACGC
TGAAAAGCTACAAGGGAGAAGCATATGACGAAGAATTGAAGGAGCAATCCAAGCAGCTGCGCACGGAAGCAAAAAAGATT
GTTGAAAAGCTGAAAGAAGAATATTTCTCGCATCAGCCGGCAAGCTATATTAGCAGCATGAAAGAGATGAAGGAACTGAT
TGCTGCGCTTGCTCGCTTAGTCGGAAGATTTGCTGGCAAGTTTCAGGCCGTGAAACGCGAAAAAGGAATTGTTGATTTTA
GTGATTTAGAGCATTTAGCACTAGCTATTTTAACTGGGGAGGAAGGCGGTCCTTCTGAAGCAGCTCAGGCTTATCAGGAG
CAATTTAAAGAGATTTTCCTCGATGAATACCAGGATTTTAATATTGTTCAAGAATCGATTATTCAGCTCGTAAAAACAGG
AGATGAAGAGAACGGCAATGTCTTTATGGTCGGGGACGTAAAGCAAAGTATTTACCGTTTCCGCCAAGCAGAGCCTAATT
TGTTTTTGAGCAAGTATCGGCGCTTTTCAACAGACGGCCAATCTAGCGGCTTGCGCATTGATTTATCGCAAAACTTCCGC
AGCCGTGCAGAAGTGCTGGCGGGCACAAACTTTCTGTTCAAGCAGATTATGGATGTGAAAATTGGCGAAATTGAATATGA
CCGTCAAGCTGAATTGGTGAAGGGAGCTGCTTATCCGGAAGAGAAGCCGTTTCCGGTGGAGGTGACGATTATCGACCAGG
AAGGGGCAGGGCAGTCCGAGGATCCTGATATGAAAGACGCGGCCCAGTCTGAACTTGAGGCGAGATGGATAGCCGGGAAA
ATCAAGGATCTTGTCGAGTCTAGACATCCTGTCTATGATCCGAAAACTAAAAGTGAGCGGCCGGTTGAATATCGCGATAT
CGTCATCTTGCTGCGTTCGATGACGTGGGCCGGTGTAATCAAGGAAGAATTTAAGCGTGCCGGCATTCCGCTTCACGCAA
AAGTTGCTGGCGGCTATTTCCAAACGATGGAGATTGCTGTCATGCTTTCTTTGCTGAAGGTCATCGACAATCCGTACCAA
GATATCCCGCTCGCTTCCGTGCTGCGCTCTCCCATTATGCGCTGTACGGAAAACGATCTTGCTGTTATCCGGCTGGCCTC
CAAGTCAGGCTCTTACTATGAAGCAGCGAAGGCGTTTGCGGCATCCCGGCCGGAAGTGCGTTATGAGGAACTGCATGAGA
AGGTGAGCAAGTTTCTTGAACAGTTAGCCGAGTGGCGTTCGCTTAGCCGCTATCAAGCTTTGTCTGCTCTCGTATGGCAG
CTGTATCATGACACCCATTTTTATGATTTTGTGGGCGGCATGCCGGGCGGAAAGCAGCGCCAGGCAAACTTGCGCGCCTT
ATATGACCGGGTGCGGCAATACGAAGAAACTTCTTTTCGGGGATTATTCCGCTTTCTCCGCTTTGTGGAACGGATGCAGG
AACGCGGAGAAGACCTTGGGGAGGCCGGCGCTTTAAGTGAGCAGGAAGATGTCGTCCGCCTGATGACGATCCACGCTTCG
AAAGGGCTGGAGTTTCCTGTTGTTTTCGTCGCCGGCTTGAACCGGTCGTTTAATATGACGGATTTGAATAAGAGCTATTT
GCTTGATAAAGAGCTCGGCATTGGCACTAAATATGTGAATGCCGAAAAGAGAATCAGCTATCCGTCATTGCCGCAGCTGG
CACTAAAGCGTAAAAAACGATTTGAGCTGCTTGCTGAAGAAATGCGTGTTTTATACGTGGCGTTAACGAGGGCAAAGGAA
AAACTGTTTTTGCTCGGTTCAGTGAAAAGCGCAGCTAAAGAAGCGGCCAAGTGGGAAAGGGCTGCGCGGCATCCGGATTG
GCTTTTGGGCAGTGCAGCGAGAGTTTCAGCTAAATCTTATCTTGATTGGGTTGGTCCGGCGCTTGTCCGCCATCTTGATT
ATACAAAAGCAGAGCAAACTCGTCCTGCTGTGTTTGTGGATCATCCGTCAAGATGGTCCATTCAAACTGTCGACGCGGAG
GCATTGCAGCCGGCATCGCTTGAGCAGGCTGAGGCAAGCGGCGGTTTGCTTGAAAGGGTGCAAGCTTTTGAGCCCGTTCC
TGCTTCTTCAACCTTTAAAGAGGAAATAACAGCGCGGATGAATTGGGCCTATGAATATGAAGCTGCCACTCACTTGCGGT
CGAAGCAGTCTGTGTCCGATTTAAAACGGATGAATGAGATTTTCAATGAGGGAGCAGACAATGACCTTGCCCGCCATTAT
CAGCGGCCGGTTTTTCGCCGTCCGTCCTTTATGCAGACAAAATCGATGACACCGGCTGAGGCCGGAACAGCTCTTCACAC
GGTGATGCAGCATGTGCCGCTTCAGGAAGCTCCAGATGCGGCAGAGGTCGGGCAGCTGTTGCGCACGCTCGTTGCCCGCG
AATTGCTGACAGAAGAGCAAGCCGCGTCTGTTGACCCGGAAAAGATAGCTTTCTTTTTCCGTACAGATATTGGTCGGGAG
CTGCTGCAGGCGGAAGAGATTCACCGGGAAACACCATTTAATATGGCTATAGATGCCGGTGAGATTTATCCTGATTGGAA
AGGTCCTGAAGAAGCAGTTTTAGTTCAGGGTGTGATTGATTGTTTATTTGAAAAAGATGGAAAACTGTATTTGCTTGATT
ATAAAACCGATCAAATTACAGGCCGCTTTCCTGGAGGCATGGAACAAGCCCGTCCTCTGTTAAAGAAGCGTTATGAGGTG
CAAATTCAATTGTATGCCCAGGCTGTAGAACGCATCTGGAAGCGAAAAGTAGATTATAAGCTCCTGTTCTTTTTTGATGG
AGGTTATTTGCTTCCGATTGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1038-1216)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(15-462)

UvrD-like helicase C-terminal domain (UvrD_C)

(473-884)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

54.143

100

0.545