Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   K7X51_RS01555 Genome accession   NZ_CP082359
Coordinates   344909..346282 (+) Length   457 a.a.
NCBI ID   WP_004562734.1    Uniprot ID   A0A806TIP1
Organism   Limosilactobacillus fermentum strain ACA-DC 179     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 323427..347448 344909..346282 within 0


Gene organization within MGE regions


Location: 323427..347448
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K7X51_RS01435 (K7X51_01435) - 323427..323957 (-) 531 WP_015639482.1 DapH/DapD/GlmU-related protein -
  K7X51_RS10915 - 324055..324369 (+) 315 Protein_281 (deoxy)nucleoside triphosphate pyrophosphohydrolase -
  K7X51_RS10920 - 324457..324885 (+) 429 WP_226557723.1 thioesterase family protein -
  K7X51_RS10925 - 324970..325074 (+) 105 WP_021350099.1 NAD(P)H-dependent oxidoreductase -
  K7X51_RS01445 (K7X51_01445) - 325031..325504 (+) 474 WP_100184382.1 flavodoxin family protein -
  K7X51_RS01450 (K7X51_01450) - 325808..327262 (+) 1455 WP_003681354.1 APC family permease -
  K7X51_RS01455 (K7X51_01455) - 327243..328595 (+) 1353 WP_223045515.1 aspartate aminotransferase family protein -
  K7X51_RS01460 (K7X51_01460) - 328941..330227 (+) 1287 WP_223045516.1 ISL3 family transposase -
  K7X51_RS01465 (K7X51_01465) - 330375..331174 (+) 800 WP_372487746.1 IS5 family transposase -
  K7X51_RS01470 (K7X51_01470) - 331185..331319 (-) 135 Protein_289 helix-turn-helix domain-containing protein -
  K7X51_RS01475 (K7X51_01475) - 331536..332723 (+) 1188 WP_045353779.1 MFS transporter -
  K7X51_RS01480 (K7X51_01480) nadC 332720..333559 (+) 840 WP_100184397.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K7X51_RS01485 (K7X51_01485) - 333863..335101 (-) 1239 Protein_292 ISL3-like element IS1165 family transposase -
  K7X51_RS01490 (K7X51_01490) - 335270..335548 (-) 279 WP_100184398.1 hypothetical protein -
  K7X51_RS01500 (K7X51_01500) - 336584..337468 (-) 885 WP_107760586.1 IS3 family transposase -
  K7X51_RS01505 (K7X51_01505) - 337456..338169 (-) 714 WP_003684319.1 helix-turn-helix domain-containing protein -
  K7X51_RS01510 (K7X51_01510) - 338352..339539 (+) 1188 WP_014562705.1 IS256 family transposase -
  K7X51_RS01515 (K7X51_01515) tnpA 339590..340003 (+) 414 Protein_298 IS200/IS605 family transposase -
  K7X51_RS01520 (K7X51_01520) - 340000..341163 (+) 1164 WP_003684331.1 RNA-guided endonuclease TnpB family protein -
  K7X51_RS01525 (K7X51_01525) - 341458..341706 (+) 249 WP_003681357.1 hypothetical protein -
  K7X51_RS01530 (K7X51_01530) - 341647..341847 (-) 201 Protein_301 AEC family transporter -
  K7X51_RS01535 (K7X51_01535) tnpA 341895..342347 (-) 453 WP_012390701.1 IS200/IS605 family transposase -
  K7X51_RS01540 (K7X51_01540) - 342426..343676 (+) 1251 WP_086325846.1 RNA-guided endonuclease TnpB family protein -
  K7X51_RS01545 (K7X51_01545) - 343831..344157 (-) 327 WP_035437471.1 GNAT family N-acetyltransferase -
  K7X51_RS01550 (K7X51_01550) - 344357..344896 (+) 540 WP_004563338.1 dUTP diphosphatase -
  K7X51_RS01555 (K7X51_01555) radA 344909..346282 (+) 1374 WP_004562734.1 DNA repair protein RadA Machinery gene
  K7X51_RS01560 (K7X51_01560) - 346315..347448 (+) 1134 WP_004562735.1 PIN/TRAM domain-containing protein -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49381.79 Da        Isoelectric Point: 8.7358

>NTDB_id=601756 K7X51_RS01555 WP_004562734.1 344909..346282(+) (radA) [Limosilactobacillus fermentum strain ACA-DC 179]
MAKAKTHFVCQNCGYNSPRYLGRCPNCGQWSTLVEEVEQASTPAAKNATATLTGIVARPQRIQEIDTKKTPRVKTRLNEL
NRVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSGQLSVEHHKVLYVSGEESASQIKLRAQRLDVSGEDFYIYPETNMEAIR
ATIDSIQPEFVIIDSVQTMQAADVTSAIGSVSQIRAVTAQLMQIAKGQNITVFVVGHVTKGGVLAGPKILEHMVDTVLYF
EGDLHHTYRILRSVKNRFGSTNELGIFEMNTRGLTEVANPSEIFLEERLKDATGSAVVVSLEGTRPILVEIQALITPTVF
GNAQRTATGLNRNRVSLIMAVLEKRANLLLQNQDAYLKAAGGVKLDEPAIDLAIAVAIASSYRDKGTRPTDAFVGEVGLT
GEIRRVSRIEQRVAEAAKLGFKRILVPKNNLAGWNPPQDIEVVGVTTLGEALKIALG

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=601756 K7X51_RS01555 WP_004562734.1 344909..346282(+) (radA) [Limosilactobacillus fermentum strain ACA-DC 179]
ATGGCAAAAGCCAAAACACACTTTGTCTGCCAAAACTGCGGTTACAATTCACCGCGCTATTTAGGGCGGTGTCCCAATTG
TGGCCAGTGGAGTACCTTAGTTGAAGAAGTCGAGCAGGCAAGTACCCCGGCGGCTAAAAACGCTACGGCAACCTTAACCG
GGATTGTGGCCCGGCCCCAGCGAATCCAAGAAATTGACACCAAAAAAACGCCCCGGGTTAAGACCCGCTTAAATGAGTTA
AACCGGGTGCTAGGTGGCGGAATTGTACCCGGCTCGTTGGTCTTAATTGGTGGGGATCCCGGGATCGGAAAGTCCACCCT
GCTCTTACAAGTGTCGGGGCAACTGAGCGTGGAACACCACAAGGTTCTTTACGTTTCCGGGGAAGAATCGGCCTCCCAAA
TTAAATTGCGGGCCCAACGCTTAGACGTTTCCGGCGAGGACTTTTATATCTACCCGGAAACCAACATGGAAGCCATCCGG
GCGACGATTGATTCCATTCAGCCCGAGTTTGTAATCATTGACTCGGTTCAGACGATGCAGGCGGCCGACGTGACCTCGGC
GATTGGGTCGGTCTCCCAAATCCGGGCGGTCACCGCCCAGTTGATGCAGATTGCCAAGGGGCAAAACATCACGGTCTTCG
TGGTTGGTCACGTCACCAAGGGTGGGGTCTTAGCCGGGCCCAAGATCTTGGAACACATGGTTGACACGGTGCTGTACTTT
GAAGGGGACCTCCACCACACCTACCGGATTTTACGGTCGGTCAAAAACCGGTTTGGGTCCACCAATGAGTTAGGGATCTT
CGAAATGAATACCCGCGGTTTGACCGAGGTCGCAAACCCCTCGGAAATCTTTTTGGAAGAGCGGCTTAAGGATGCCACCG
GGTCGGCCGTGGTCGTATCCCTGGAGGGAACCCGACCGATCCTGGTTGAAATCCAGGCCTTGATCACGCCAACCGTCTTT
GGAAACGCCCAAAGAACGGCGACAGGTTTAAACCGCAACCGGGTTTCCTTGATCATGGCGGTCCTAGAAAAACGGGCGAA
CCTGCTCTTACAAAACCAGGATGCCTACTTGAAGGCGGCCGGGGGCGTTAAGTTAGACGAGCCGGCCATCGACCTTGCGA
TCGCGGTGGCAATTGCTTCTTCTTACCGGGATAAGGGGACCCGGCCGACCGACGCCTTCGTCGGTGAAGTTGGCTTAACC
GGGGAGATCCGTCGGGTGAGCCGAATTGAACAGCGGGTGGCCGAAGCCGCTAAACTAGGCTTTAAGCGGATTTTAGTGCC
CAAAAACAATTTAGCGGGTTGGAACCCGCCGCAAGACATCGAAGTCGTGGGGGTAACGACCCTTGGCGAAGCACTTAAAA
TTGCCCTTGGATAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

AAA domain (AAA_25)

(64-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A806TIP1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

68.212

99.125

0.676
  radA Streptococcus mitis NCTC 12261

67.991

99.125

0.674
  radA Streptococcus pneumoniae Rx1

67.991

99.125

0.674
  radA Streptococcus pneumoniae D39

67.991

99.125

0.674
  radA Streptococcus pneumoniae R6

67.991

99.125

0.674
  radA Streptococcus pneumoniae TIGR4

67.991

99.125

0.674
  radA Bacillus subtilis subsp. subtilis str. 168

63.676

100

0.637