Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   K7H19_RS06970 Genome accession   NZ_CP082323
Coordinates   1319399..1320022 (+) Length   207 a.a.
NCBI ID   WP_011228637.1    Uniprot ID   A0A3P4AP50
Organism   Thermus thermophilus strain N-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1314399..1325022
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K7H19_RS06950 (K7H19_06950) - 1315130..1316536 (+) 1407 WP_011228635.1 HD-GYP domain-containing protein -
  K7H19_RS06955 (K7H19_06955) - 1316587..1317078 (+) 492 WP_011173430.1 DUF5317 domain-containing protein -
  K7H19_RS06960 (K7H19_06960) - 1317133..1318137 (+) 1005 WP_011173431.1 homoisocitrate dehydrogenase -
  K7H19_RS06965 (K7H19_06965) pilM 1318273..1319406 (+) 1134 WP_011228636.1 type IV pilus assembly protein PilM Machinery gene
  K7H19_RS06970 (K7H19_06970) pilN 1319399..1320022 (+) 624 WP_011228637.1 competence protein Machinery gene
  K7H19_RS06975 (K7H19_06975) pilO 1320012..1320593 (+) 582 WP_011173434.1 type 4a pilus biogenesis protein PilO Machinery gene
  K7H19_RS06980 (K7H19_06980) pilW 1320590..1321468 (+) 879 WP_011228638.1 competence protein Machinery gene
  K7H19_RS06985 (K7H19_06985) pilQ 1321465..1323738 (+) 2274 WP_011228639.1 secretin N-terminal domain-containing protein Machinery gene
  K7H19_RS06990 (K7H19_06990) aroC 1323797..1324948 (+) 1152 WP_011228640.1 chorismate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 23063.56 Da        Isoelectric Point: 9.7530

>NTDB_id=601476 K7H19_RS06970 WP_011228637.1 1319399..1320022(+) (pilN) [Thermus thermophilus strain N-1]
MIRLNLLPKNLRRRVEPGWWRLLALLFALVVLGVLGLVHYTAYTELSLAKAERDQLQAEVEALRPFIAELGRLQEERKAL
EALLAIREGLEKNAVPWSQYLAAFINQIPRAGGRLEVALRSVSARALSEEEAARLAQEGTYDGKRIRVEFALQGEALSRE
ALVRFIRAFETSPRFGIEFQGASLDEGRGLYTFSARVGVTGGESGAR

Nucleotide


Download         Length: 624 bp        

>NTDB_id=601476 K7H19_RS06970 WP_011228637.1 1319399..1320022(+) (pilN) [Thermus thermophilus strain N-1]
TTGATTAGGCTGAACCTTCTCCCCAAAAACCTCCGGCGCCGGGTGGAGCCGGGCTGGTGGCGCCTCCTCGCCCTCCTCTT
CGCCCTCGTGGTCCTCGGGGTCTTGGGGCTCGTCCACTACACCGCCTACACCGAGCTCTCCCTGGCCAAGGCGGAGCGGG
ACCAGCTCCAGGCGGAGGTGGAGGCCTTGAGGCCCTTCATCGCCGAGCTCGGCCGCCTGCAGGAGGAGCGTAAGGCCCTC
GAGGCCCTCCTCGCCATCCGGGAAGGCCTGGAGAAAAACGCCGTGCCCTGGTCCCAGTACCTGGCCGCCTTCATCAACCA
GATCCCCAGGGCGGGGGGGCGCCTCGAGGTGGCCCTCCGCTCGGTGAGCGCCCGCGCCCTCTCGGAAGAGGAGGCCGCCC
GCCTGGCCCAGGAAGGCACCTACGACGGCAAGCGGATCCGGGTGGAGTTCGCCCTCCAGGGGGAGGCCCTGAGCCGGGAG
GCCCTGGTCCGGTTCATCCGGGCCTTTGAGACCTCCCCCCGGTTCGGCATTGAGTTCCAGGGGGCCTCCCTGGACGAGGG
CCGGGGGCTTTACACCTTCAGCGCCCGCGTGGGCGTGACGGGGGGTGAAAGCGGTGCTCGCTAG

Domains

Predicted by InterproScan.

PilN biogenesis protein dimerization domain (PilN_bio_d)

(97-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3P4AP50

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Thermus thermophilus HB27

99.517

100

0.995