Detailed information NTG0601232

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NE421_RS05115
• Genome accession: NZ_CP098791
• Coordinates: 1047536..1048654 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A086HYC5
• Organism: Acinetobacter baumannii strain 280820
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1042536..1053654

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NE421_RS05085 (NE421_05085)	-	1043366..1044214 (+)	849	WP_000336551.1	class II glutamine amidotransferase	-
NE421_RS05090 (NE421_05090)	-	1044467..1044922 (+)	456	WP_000782976.1	bacteriohemerythrin	-
NE421_RS05095 (NE421_05095)	-	1045075..1046142 (+)	1068	WP_002084046.1	hypothetical protein	-
NE421_RS05100 (NE421_05100)	-	1046142..1046465 (+)	324	WP_000442585.1	RnfH family protein	-
NE421_RS05105 (NE421_05105)	bamE	1046520..1046918 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
NE421_RS05110 (NE421_05110)	fur	1047030..1047467 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
NE421_RS05115 (NE421_05115)	pilU	1047536..1048654 (-)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NE421_RS05120 (NE421_05120)	pilT	1048682..1049719 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
NE421_RS05125 (NE421_05125)	-	1049846..1050538 (+)	693	WP_031965550.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=601232 NE421_RS05115 WP_000347036.1 1047536..1048654(-) (pilU) [Acinetobacter baumannii strain 280820]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=601232 NE421_RS05115 WP_000347036.1 1047536..1048654(-) (pilU) [Acinetobacter baumannii strain 280820]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAGAAATCATCGGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCACCAGATGTCATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTTGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTACATGGATTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A086HYC5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392