Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   NEH83_RS04950 Genome accession   NZ_CP098783
Coordinates   1179095..1180180 (-) Length   361 a.a.
NCBI ID   WP_030085923.1    Uniprot ID   A0ABW2MNP9
Organism   Streptomyces sp. JUS-F4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1174095..1185180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NEH83_RS04930 (NEH83_04935) - 1174851..1175402 (-) 552 WP_030633279.1 adenine phosphoribosyltransferase -
  NEH83_RS04935 (NEH83_04940) secF 1175406..1176491 (-) 1086 WP_302212216.1 protein translocase subunit SecF -
  NEH83_RS04940 (NEH83_04945) secD 1176493..1178271 (-) 1779 WP_030085928.1 protein translocase subunit SecD -
  NEH83_RS04945 (NEH83_04950) yajC 1178422..1178928 (-) 507 WP_030085926.1 preprotein translocase subunit YajC -
  NEH83_RS04950 (NEH83_04955) ruvB 1179095..1180180 (-) 1086 WP_030085923.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  NEH83_RS04955 (NEH83_04960) ruvA 1180210..1180836 (-) 627 WP_030085921.1 Holliday junction branch migration protein RuvA -
  NEH83_RS04960 (NEH83_04965) ruvC 1180833..1181429 (-) 597 WP_030085919.1 crossover junction endodeoxyribonuclease RuvC -
  NEH83_RS04965 (NEH83_04970) - 1181585..1182337 (-) 753 WP_030085918.1 YebC/PmpR family DNA-binding transcriptional regulator -
  NEH83_RS04970 (NEH83_04975) pdxT 1182399..1182992 (-) 594 WP_086778118.1 pyridoxal 5'-phosphate synthase glutaminase subunit PdxT -
  NEH83_RS04975 (NEH83_04980) pdxS 1182999..1183919 (-) 921 WP_003970325.1 pyridoxal 5'-phosphate synthase lyase subunit PdxS -
  NEH83_RS04980 (NEH83_04985) - 1184089..1184637 (-) 549 WP_302212223.1 hypothetical protein -

Sequence

Protein


Download         Length: 361 a.a.        Molecular weight: 38564.06 Da        Isoelectric Point: 4.7885

>NTDB_id=601040 NEH83_RS04950 WP_030085923.1 1179095..1180180(-) (ruvB) [Streptomyces sp. JUS-F4]
MNWDETGPETDEPTGPVLDDRLVDADADGEDTAVEAALRPKDLEEFVGQEKVREQLDLVLKAARARGATADHVLLSGAPG
LGKTTLSMIIAAEMNAPIRITSGPAIQHAGDLAAILSSLQEGEVLFLDEIHRMSRPAEEMLYMAMEDFRVDVIVGKGPGA
TAIPLELPPFTLVGATTRAGLLPPPLRDRFGFTGHMEFYAPAELERVIHRSARLLDVAIDAEGAAEIAGRSRGTPRIANR
LLRRVRDYAQVKAEGAIDREIAAAALKVYEVDARGLDRLDRAVLGALLKLFGGGPVGLSTLAVAVGEERETVEEVAEPFL
VREGLLARTPRGRVATPAAWAHLGLVPPQHGAKGQQGLFGA

Nucleotide


Download         Length: 1086 bp        

>NTDB_id=601040 NEH83_RS04950 WP_030085923.1 1179095..1180180(-) (ruvB) [Streptomyces sp. JUS-F4]
ATGAACTGGGACGAGACCGGACCCGAGACCGACGAGCCCACCGGCCCGGTCCTCGACGACCGGCTGGTCGACGCCGACGC
GGACGGTGAGGACACCGCGGTCGAGGCGGCCCTGCGCCCCAAGGACCTGGAGGAGTTCGTCGGCCAGGAGAAGGTCCGCG
AACAGCTCGACCTGGTCCTCAAGGCCGCCCGCGCCCGCGGCGCCACCGCCGACCACGTGCTGCTCTCCGGCGCCCCCGGC
CTCGGCAAGACCACCCTCTCGATGATCATCGCCGCCGAGATGAACGCCCCGATCCGCATCACCTCGGGCCCCGCCATCCA
GCACGCCGGCGACCTCGCGGCGATCCTCTCCTCCCTCCAGGAGGGCGAGGTCCTCTTCCTCGACGAGATCCACCGCATGT
CCCGGCCCGCCGAGGAGATGCTCTACATGGCGATGGAGGACTTCCGCGTCGACGTCATCGTCGGCAAGGGCCCCGGCGCC
ACCGCGATCCCGCTGGAGCTGCCCCCCTTCACCCTGGTCGGGGCCACCACCCGGGCCGGACTGCTGCCGCCCCCGCTGCG
CGACCGCTTCGGCTTCACCGGGCACATGGAGTTCTACGCCCCCGCCGAGCTGGAGCGCGTCATCCACCGCTCCGCCCGCC
TCCTCGACGTCGCCATCGACGCGGAGGGCGCCGCCGAGATCGCCGGACGCTCCCGCGGCACCCCCCGTATCGCCAACCGC
CTGCTGCGCCGGGTCCGCGACTACGCCCAGGTCAAGGCGGAGGGAGCGATCGACCGGGAGATCGCCGCGGCCGCCCTCAA
GGTGTACGAGGTTGACGCCCGCGGACTCGATCGGCTGGACCGTGCCGTCCTCGGCGCGCTGCTGAAGCTCTTCGGCGGCG
GGCCGGTCGGCCTGTCCACCCTGGCGGTCGCCGTGGGGGAGGAGCGGGAGACCGTCGAGGAGGTCGCCGAGCCCTTCCTC
GTACGGGAAGGACTGCTGGCCAGGACGCCGCGCGGCCGGGTCGCGACCCCCGCTGCCTGGGCGCATCTGGGCCTGGTCCC
GCCGCAGCACGGAGCAAAGGGACAACAGGGTCTGTTCGGGGCGTGA

Domains

Predicted by InterProScan.

RuvB C-terminal winged helix domain (WHD_RuvB)

(274-343)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(38-196)

RuvB AAA lid domain (AAA_lid_4)

(199-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

52.553

92.244

0.485
  ruvB Streptococcus pneumoniae TIGR4

52.778

89.751

0.474
  ruvB Streptococcus pneumoniae R6

52.778

89.751

0.474
  ruvB Streptococcus pneumoniae D39

52.778

89.751

0.474
  ruvB Synechocystis sp. PCC 6803

53.503

86.981

0.465
  ruvB Helicobacter pylori 26695

50.625

88.643

0.449