Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   K7I17_RS05230 Genome accession   NZ_CP082279
Coordinates   1030400..1034104 (+) Length   1234 a.a.
NCBI ID   WP_014470010.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain Bam1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1025400..1039104
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K7I17_RS05215 (K7I17_05215) - 1025463..1025990 (-) 528 WP_013351758.1 ferritin-like domain-containing protein -
  K7I17_RS05220 (K7I17_05220) - 1026059..1026772 (-) 714 WP_013351759.1 DUF421 domain-containing protein -
  K7I17_RS05225 (K7I17_05225) addB 1026913..1030413 (+) 3501 WP_014470009.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  K7I17_RS05230 (K7I17_05230) addA 1030400..1034104 (+) 3705 WP_014470010.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  K7I17_RS05235 (K7I17_05235) sbcD 1034171..1035343 (+) 1173 WP_013351762.1 exonuclease subunit SbcD -
  K7I17_RS05240 (K7I17_05240) sbcC 1035340..1038732 (+) 3393 WP_014470011.1 exonuclease subunit SbcC -
  K7I17_RS05245 (K7I17_05245) - 1038748..1039047 (+) 300 WP_013351764.1 hypothetical protein -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141279.77 Da        Isoelectric Point: 5.1885

>NTDB_id=600917 K7I17_RS05230 WP_014470010.1 1030400..1034104(+) (addA) [Bacillus amyloliquefaciens strain Bam1]
MQIPKPKDSIWTDDQWSAIVSSGRDILVAAAAGSGKTAVLVERMIRKITAEEEPVDVDRLLVVTFTNASAAEMKHRIAEA
LEKELAKNPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYMIDLDPGFRMADQTEGELLGDEVLDELFEDEYAKGNQA
FFELVDRYTTDRHDLDLQDLVKRVYEYSRSHPDPEAWLKSFVRLYDVTEESKMEELPFYEYVREDAEMALFGAKQKLEKA
LELTKAPGGPAPRADNVLDDLQQIEELISCRHDFKALYERVPAVTFKRAKAVKGDEFDKALLDEATDLRNGAKKLIEKLK
TDYFTRSPQDHLKSLKDMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTARDEEGRRVPSEAAVYYQDQ
FHEVLVDEYQDTNLVQESILQLVKSGNEEAGNLFMVGDVKQSIYRFRLAEPLLFLGKYKRFTEDGEGAGQKIDLNQNFRS
RSDILDSTNFLFKQLMGGKIGEVDYDEQAALKLGASYPPNDAAKTELLLIDSADDADSPEDAEDFETVHWEAKAIAAEIR
KLVSSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQLMEELKNQGIPVYANLTSGYFEAVEVAAALSVLKVIDNPYQDI
PLASVLRSPIVGCDENELALIRLEKKKAPFYEALKAYIANGDRHDDLYQKLRTFYDSLQKWRSFSTNHSVSELIWEVYRD
TGYFDYAGGMPGGKQRQANLRVLYDRARSYEATAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRSFNMMDLNKSYLLDKELGFGTKYIHPELRISYPTLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKNREKQLAKWQAQADRADWLLSEFDRYQASSYLDFIGPALIRHSDMEAHRAPGLLAPEDIAHDPARFHIRMLQQSE
LLEDSPEERAEEKSKRLKAIQHGEPIPDSFSFDDQARRLLEWEYPYRKLTAIRTKQSVSELKRKQEYEDEYSGRSLIKPS
GDTLLYKRPGFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTAEEAERTVRMLYEKELLTEEQQDAIDIDEVIQFFGTEIGK
DLVGALRIDREVPFSMALPASEVYKDAETAGEPLLVQGIIDCLYETADGLYLLDYKTDRIEGKFRNGFEGAAPILQKRYE
TQIELYTKAVEQITKTNVKRRALYFFDGGHVLTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=600917 K7I17_RS05230 WP_014470010.1 1030400..1034104(+) (addA) [Bacillus amyloliquefaciens strain Bam1]
ATGCAAATTCCTAAACCGAAAGACAGCATATGGACGGATGACCAATGGAGCGCCATCGTTTCTTCCGGCCGCGATATTCT
GGTCGCTGCCGCAGCCGGATCAGGTAAAACGGCCGTGCTTGTAGAGCGGATGATCAGAAAGATAACCGCGGAAGAAGAGC
CGGTTGATGTTGACAGGCTCCTTGTGGTGACATTTACAAACGCCTCAGCAGCGGAAATGAAGCATCGGATCGCTGAAGCA
TTAGAAAAAGAACTCGCCAAAAATCCGGGGTCTCTGCATATCAGACGGCAGCTGTCTCTATTAAATCGGGCGAGCATTTC
GACGCTTCACTCTTTCTGTCTGCAGGTGCTTAAAAAATATTATTATATGATTGATCTTGACCCGGGTTTTCGGATGGCTG
ACCAGACGGAAGGCGAATTGCTCGGAGACGAAGTGCTTGATGAGCTGTTTGAGGATGAATACGCAAAAGGGAATCAGGCG
TTTTTTGAGCTTGTGGACAGATATACGACGGACAGGCATGATCTGGATCTTCAAGACCTCGTCAAGCGGGTGTATGAGTA
TTCCCGGTCGCATCCTGACCCGGAAGCGTGGCTGAAAAGCTTTGTCCGCCTATATGATGTGACAGAAGAAAGCAAAATGG
AAGAGCTGCCGTTTTACGAGTATGTGAGAGAAGATGCTGAAATGGCGCTCTTCGGGGCTAAACAAAAGCTAGAGAAGGCG
CTTGAACTGACAAAAGCGCCCGGCGGGCCGGCGCCGCGTGCTGATAATGTTCTGGATGACCTTCAGCAGATAGAAGAGCT
GATCAGCTGCCGGCATGACTTTAAGGCGCTGTATGAACGGGTTCCTGCGGTCACATTTAAACGGGCCAAGGCGGTAAAGG
GTGATGAATTTGACAAGGCGCTTTTGGACGAAGCGACTGATTTGCGAAACGGCGCGAAGAAACTGATCGAAAAGTTAAAA
ACCGATTACTTTACAAGAAGCCCGCAAGATCATTTAAAGAGTTTAAAAGACATGAAGCCTGTTATTGAAACCCTCGTTCA
GCTCGTAATATCATACGGAAAACGATTTGAAGCGGCGAAACAGGAAAAATCAATCATTGATTTCTCAGATTTGGAACATT
ACTGTCTGGCGATTTTAACTGCGAGGGATGAAGAAGGCCGGCGTGTGCCGAGTGAAGCGGCCGTTTATTATCAAGACCAA
TTCCATGAAGTTCTGGTTGATGAATATCAAGATACCAATCTTGTGCAGGAATCCATTTTGCAGCTTGTGAAAAGCGGGAA
TGAAGAGGCGGGAAATCTGTTTATGGTGGGGGACGTCAAGCAATCAATCTACCGTTTCAGACTTGCCGAGCCGCTGCTTT
TTCTCGGGAAATACAAAAGGTTTACGGAAGATGGGGAAGGCGCCGGGCAGAAAATCGACCTGAACCAAAACTTCCGAAGC
CGCTCCGATATTTTGGACAGTACGAATTTCTTGTTTAAGCAGCTGATGGGCGGCAAAATCGGCGAAGTGGACTACGATGA
ACAGGCGGCATTAAAGCTTGGCGCATCATATCCGCCGAATGACGCGGCGAAAACGGAACTGCTGCTGATTGACAGCGCTG
ATGACGCAGACAGCCCCGAGGATGCCGAAGACTTTGAAACCGTGCATTGGGAAGCGAAAGCGATAGCCGCGGAGATCCGG
AAGCTTGTCTCATCTCCGTTTAAGGTATATGACGGCAAAACAAAAACACACCGGAATATCCAGTACCGGGATATCGTGAT
TTTGCTGCGCTCCATGCCATGGGCTCCGCAATTAATGGAAGAGCTGAAAAACCAGGGCATTCCGGTTTACGCCAATTTGA
CGTCAGGTTACTTTGAAGCCGTGGAGGTGGCTGCGGCGCTTTCAGTCTTAAAGGTGATTGATAATCCGTATCAGGATATT
CCGCTTGCTTCCGTGCTGCGCTCGCCCATCGTCGGCTGTGACGAAAATGAACTCGCGCTCATACGCCTTGAAAAGAAAAA
AGCGCCGTTTTATGAAGCGCTGAAAGCGTATATAGCGAACGGTGACAGACATGACGACCTGTATCAAAAACTGAGAACAT
TTTATGACAGTCTGCAGAAGTGGCGGTCATTCTCGACGAATCATTCAGTGTCTGAGCTGATTTGGGAAGTATACCGCGAC
ACCGGATATTTTGATTATGCAGGCGGCATGCCTGGCGGAAAGCAGCGTCAGGCCAATCTGCGCGTCTTGTATGACCGGGC
GCGTTCGTATGAAGCGACGGCATTCCGCGGGTTATTCCGCTTTTTGCGGTTCATTGAACGGATGCAGGAGCGCGGCGATG
ATTTGGGGACGGCGCGGGCGCTCAGCGAGCAGGAGGATGTCGTTCGGCTGATGACGATCCACAGCAGCAAAGGACTTGAA
TTCCCCGTCGTTTTCACCGCCGGACTCGGCAGAAGTTTTAATATGATGGATCTCAACAAATCATACCTGCTTGATAAAGA
ACTCGGTTTCGGCACGAAGTATATTCATCCGGAACTTAGAATCAGCTATCCGACTCTGCCGCTTGTGGCAATGAAGAAAA
AAATGCGCAGAGAGCTTTTATCAGAAGAATTGCGCGTATTGTATGTGGCGCTGACAAGGGCGAAGGAAAAGCTGTTTCTC
GTCGGTTCCTGCAAAAACCGGGAAAAACAGCTGGCCAAATGGCAGGCGCAAGCAGACCGGGCTGATTGGCTCCTTTCTGA
ATTCGACCGCTATCAGGCGTCATCTTATTTGGACTTTATCGGTCCAGCGCTCATCCGTCACAGTGACATGGAGGCGCACA
GGGCGCCGGGGCTTTTGGCGCCGGAGGATATCGCGCACGACCCGGCGCGTTTTCACATCAGGATGCTGCAGCAAAGTGAA
TTGCTGGAAGATAGTCCTGAAGAACGTGCTGAAGAAAAGAGCAAACGTTTAAAAGCGATACAGCATGGCGAGCCGATTCC
TGATTCGTTTTCATTTGATGATCAGGCGCGCCGTCTTCTTGAATGGGAATATCCGTATCGGAAGCTGACCGCCATCAGAA
CGAAGCAGTCTGTTTCTGAGCTGAAGAGAAAGCAAGAATACGAGGATGAGTACAGCGGACGCTCTCTCATCAAACCGTCA
GGGGACACTCTTTTATACAAGCGTCCCGGCTTTATGATGAAAAAAGGGCTGACAGCCGCCGAAAAAGGGACGGCGATGCA
TACGGTGATGCAGCACATACCGCTGACGCATGTTCCGACTGCCGAAGAAGCGGAGCGGACGGTCAGGATGCTTTACGAAA
AAGAGCTGCTGACAGAAGAACAGCAGGACGCAATTGATATTGATGAAGTGATTCAGTTTTTCGGCACTGAAATCGGAAAA
GACCTTGTTGGGGCGCTGCGGATTGATCGGGAGGTGCCGTTCAGTATGGCGCTTCCGGCCAGCGAGGTGTACAAGGATGC
CGAAACGGCCGGTGAACCGCTGCTCGTCCAGGGAATTATTGACTGCCTCTATGAAACTGCGGACGGACTCTATTTGCTGG
ACTATAAAACGGATCGAATTGAGGGGAAATTCCGGAACGGATTTGAAGGCGCGGCTCCGATCCTGCAAAAAAGGTATGAA
ACACAAATCGAATTGTATACAAAAGCGGTTGAACAAATTACAAAAACGAATGTGAAAAGGCGTGCGCTCTATTTCTTCGA
TGGAGGTCACGTTCTTACACTGTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1226)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-881)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

82.091

100

0.821