Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   K6I41_RS16565 Genome accession   NZ_CP082274
Coordinates   3496373..3497173 (-) Length   266 a.a.
NCBI ID   WP_250374803.1    Uniprot ID   -
Organism   Acinetobacter sp. AS23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3491373..3502173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6I41_RS16545 (K6I41_16430) - 3492439..3493137 (-) 699 WP_004794712.1 tetratricopeptide repeat protein -
  K6I41_RS16550 (K6I41_16435) hisS 3493165..3494457 (-) 1293 WP_013198977.1 histidine--tRNA ligase -
  K6I41_RS16555 (K6I41_16440) ispG 3494454..3495569 (-) 1116 WP_004698760.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  K6I41_RS16560 (K6I41_16445) - 3495591..3496382 (-) 792 WP_005302244.1 helix-turn-helix domain-containing protein -
  K6I41_RS16565 (K6I41_16450) pilF 3496373..3497173 (-) 801 WP_250374803.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  K6I41_RS16570 (K6I41_16455) rlmN 3497189..3498424 (-) 1236 WP_004794719.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  K6I41_RS16575 (K6I41_16460) ndk 3498548..3498979 (-) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  K6I41_RS16580 (K6I41_16465) iscX 3499093..3499293 (-) 201 WP_003654869.1 Fe-S cluster assembly protein IscX -
  K6I41_RS16585 (K6I41_16470) - 3499393..3499944 (-) 552 WP_013198979.1 phosphatase PAP2 family protein -
  K6I41_RS16590 (K6I41_16475) - 3499969..3501258 (-) 1290 WP_250375804.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30097.30 Da        Isoelectric Point: 8.7553

>NTDB_id=600769 K6I41_RS16565 WP_250374803.1 3496373..3497173(-) (pilF) [Acinetobacter sp. AS23]
MSTPKLKTVLCMGVAVAFLVSGCQTTHVQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGTTLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVHTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=600769 K6I41_RS16565 WP_250374803.1 3496373..3497173(-) (pilF) [Acinetobacter sp. AS23]
TTGAGTACTCCAAAGTTAAAAACGGTATTATGTATGGGGGTTGCAGTTGCATTTCTGGTAAGTGGTTGCCAGACAACGCA
TGTGCAAAAGAAAGACCCAGAAAAAGCGGTAAAAGTACGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ATTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCAACGGCAAATATGATGATGGGTATTTTA
CTACAGCAAGAAGGTAGTAAGCCGAACTTAGAAAAAGCAGAACATTACTTTAAACGAGCAATTTCATCTGAACCAGATAA
TGCTCAAGCACATAATAACTATGGTACGTACTTGTATCAAATGGAACGCTATAATGATGCGGTCGAGCAATTTCGTATTG
CTGGTACAACATTGGGTTATGACCAACGCTATCAGGCGTTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCATACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-133)

Tetratricopeptide repeat (TPR_16)

(149-205)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.609

100

0.936
  pilF Acinetobacter baylyi ADP1

75

97.744

0.733