Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   K4042_RS02330 Genome accession   NZ_CP082269
Coordinates   478749..480182 (-) Length   477 a.a.
NCBI ID   WP_222889462.1    Uniprot ID   -
Organism   Enterobacter sp. C2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 473749..485182
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K4042_RS02300 mtgA 475206..475934 (+) 729 WP_222889460.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  K4042_RS02305 yrbL 475909..476505 (-) 597 WP_222889461.1 PhoP regulatory network protein YrbL -
  K4042_RS02310 npr 476696..476968 (-) 273 WP_042390070.1 PTS phosphocarrier protein NPr -
  K4042_RS02315 rapZ 476965..477819 (-) 855 WP_042390071.1 RNase adapter RapZ -
  K4042_RS02320 ptsN 477865..478356 (-) 492 WP_144817462.1 PTS IIA-like nitrogen regulatory protein PtsN -
  K4042_RS02325 hpf 478439..478726 (-) 288 WP_042390073.1 ribosome hibernation promoting factor -
  K4042_RS02330 rpoN/rpoN1 478749..480182 (-) 1434 WP_222889462.1 RNA polymerase factor sigma-54 Machinery gene
  K4042_RS02335 lptB 480230..480955 (-) 726 WP_042390075.1 LPS export ABC transporter ATP-binding protein -
  K4042_RS02340 lptA 480962..481516 (-) 555 WP_222889463.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  K4042_RS02345 lptC 481485..482060 (-) 576 WP_144817465.1 LPS export ABC transporter periplasmic protein LptC -
  K4042_RS02350 kdsC 482057..482623 (-) 567 WP_144817466.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  K4042_RS02355 kdsD 482637..483623 (-) 987 WP_144817467.1 arabinose-5-phosphate isomerase KdsD -
  K4042_RS02360 - 483637..484602 (-) 966 WP_222889464.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53640.44 Da        Isoelectric Point: 4.4036

>NTDB_id=600640 K4042_RS02330 WP_222889462.1 478749..480182(-) (rpoN/rpoN1) [Enterobacter sp. C2]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEIDAQQTQDTETLDSADALEQKDMPE
ELPLDASWDEIYTAGTPSGNGVDYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVSLED
ILDSMGDSNVGLDEVEAVLKRVQRFDPVGVAARDLRDCLLIQLSQFASDTPWQAEAKLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGRWMVELNGDSIPRLQINQHYAAMCSGTRNDADSQFIR
SNLQEAKWLIKSLESRNDTLLRVSRCIVEQQQAFFDKGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTLLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=600640 K4042_RS02330 WP_222889462.1 478749..480182(-) (rpoN/rpoN1) [Enterobacter sp. C2]
ATGAAGCAAGGTTTGCAACTGCGGCTAAGCCAACAGCTCGCCATGACGCCCCAACTGCAACAGGCTATTCGTCTGTTGCA
GCTCTCGACGCTGGAACTTCAGCAAGAACTTCAGCAGGCGCTGGATAGCAACCCGCTGCTGGAGCAAACCGATCTTCACG
ATGAGATAGACGCCCAGCAGACCCAGGACACCGAAACATTAGACTCCGCCGACGCCCTTGAGCAGAAAGATATGCCCGAG
GAGCTGCCGCTGGATGCCAGCTGGGATGAGATCTACACCGCCGGTACGCCGTCGGGCAACGGCGTAGACTACCAGGACGA
CGAGCTTCCCGTCTACCAGGGCGAGACTACCCAGTCACTCCAGGACTACCTGATGTGGCAGGTGGAGCTGACCCCCTTCT
CTGATACCGATCGCGCTATTGCCACCTCCATTGTCGACGCCGTGGATGAGACGGGCTACCTGACCGTCTCGCTGGAGGAT
ATCCTCGACAGTATGGGCGATAGCAACGTTGGGCTGGACGAGGTCGAAGCGGTGCTGAAACGCGTCCAGCGTTTCGATCC
GGTGGGCGTAGCGGCAAGAGACCTGCGCGACTGCCTGCTGATTCAGCTCTCCCAGTTCGCCAGCGATACGCCGTGGCAGG
CCGAAGCCAAACTGATCATCAGCGATCACCTCGATCTGCTGGCGAACCACGATTTCCGCACCCTGATGCGCGTGACCCGC
CTGAAGGAAGAGGTGCTGAAAGAGGCGGTGAACCTGATCCAGTCGCTGGATCCGCGCCCTGGCCAGTCGATCCAGACCGG
CGAGCCGGAGTATGTGATCCCCGACGTGCTGGTGCGCAAGCATAACGGCCGCTGGATGGTCGAGCTGAACGGTGACAGCA
TTCCGCGTTTGCAGATCAACCAGCACTACGCCGCCATGTGCAGCGGCACGCGTAACGATGCCGATAGCCAGTTTATCCGC
AGCAATCTTCAGGAAGCCAAATGGCTGATTAAGAGCCTGGAGAGCCGCAACGATACCCTGCTGCGCGTCAGCCGCTGCAT
CGTTGAACAGCAGCAGGCCTTTTTCGACAAAGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCACGAATCCACCATTTCACGCGTAACAACCCAGAAGTACCTGCACAGCCCGAGAGGTATTTTTGAGCTTAAA
TACTTTTTCTCCAGCCACGTGAATACCGAAGGCGGCGGCGAAGCCTCGTCGACGGCTATTCGCGCGCTGGTGAAGAAGCT
AATAGCCGCAGAAAACCCTGCGAAACCACTGAGTGACAGTAAGTTGACCACCCTGCTGTCCGACCAGGGTATCATGGTGG
CTCGCCGTACCGTTGCGAAGTATCGAGAGTCTTTATCCATCCCGCCGTCTAACCAGCGCAAGCAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.545

100

0.482