Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   K4A81_RS09030 Genome accession   NZ_CP082262
Coordinates   1856274..1856708 (+) Length   144 a.a.
NCBI ID   WP_021493650.1    Uniprot ID   -
Organism   Bacillus velezensis strain BIM B-454D     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1851274..1861708
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K4A81_RS09025 (K4A81_09025) - 1854774..1855985 (-) 1212 WP_025649439.1 cytochrome P450 -
  K4A81_RS09030 (K4A81_09030) nucA/comI 1856274..1856708 (+) 435 WP_021493650.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  K4A81_RS09035 (K4A81_09035) - 1856768..1857523 (+) 756 WP_095060096.1 YoaK family protein -
  K4A81_RS09040 (K4A81_09040) - 1857557..1857919 (-) 363 WP_007410383.1 hypothetical protein -
  K4A81_RS09045 (K4A81_09045) - 1858112..1859440 (-) 1329 WP_014417826.1 S8 family peptidase -
  K4A81_RS09050 (K4A81_09050) - 1859619..1859831 (+) 213 WP_103526887.1 hypothetical protein -
  K4A81_RS09055 (K4A81_09055) - 1860108..1860815 (+) 708 WP_014417828.1 poly-gamma-glutamate hydrolase family protein -
  K4A81_RS09060 (K4A81_09060) - 1860875..1861327 (+) 453 WP_014305039.1 OsmC family protein -
  K4A81_RS09065 (K4A81_09065) - 1861341..1861694 (-) 354 WP_007410379.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15480.42 Da        Isoelectric Point: 7.2418

>NTDB_id=600555 K4A81_RS09030 WP_021493650.1 1856274..1856708(+) (nucA/comI) [Bacillus velezensis strain BIM B-454D]
MNAFMKWAASLLLVISLQVGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=600555 K4A81_RS09030 WP_021493650.1 1856274..1856708(+) (nucA/comI) [Bacillus velezensis strain BIM B-454D]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGGTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATACT
CCGACGGCACGAGAGTACTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486