Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ABWED_RS12555 Genome accession   NZ_CP082143
Coordinates   2593648..2594448 (-) Length   266 a.a.
NCBI ID   WP_005103248.1    Uniprot ID   N9QCA1
Organism   Acinetobacter lwoffii strain ED23-35     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2588648..2599448
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABWED_RS12535 (ABWED_2603) - 2589693..2590391 (-) 699 WP_005103250.1 tetratricopeptide repeat protein -
  ABWED_RS12540 (ABWED_2604) hisS 2590422..2591717 (-) 1296 WP_374956022.1 histidine--tRNA ligase -
  ABWED_RS12545 (ABWED_2605) ispG 2591721..2592842 (-) 1122 WP_004278355.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ABWED_RS12550 (ABWED_2606) - 2592866..2593657 (-) 792 WP_004647463.1 DUF4115 domain-containing protein -
  ABWED_RS12555 (ABWED_2607) pilF 2593648..2594448 (-) 801 WP_005103248.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ABWED_RS12560 (ABWED_2608) rlmN 2594463..2595698 (-) 1236 WP_004278358.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  ABWED_RS12565 (ABWED_2609) ndk 2595821..2596252 (-) 432 WP_004647460.1 nucleoside-diphosphate kinase -
  ABWED_RS12570 (ABWED_2610) iscX 2596363..2596563 (-) 201 WP_004278360.1 Fe-S cluster assembly protein IscX -
  ABWED_RS12575 (ABWED_2611) - 2596651..2597202 (-) 552 WP_004278361.1 phosphatase PAP2 family protein -
  ABWED_RS12580 (ABWED_2612) - 2597223..2598503 (-) 1281 WP_005103246.1 glycosyltransferase family 1 protein -
  ABWED_RS12585 (ABWED_2613) - 2598745..2599155 (-) 411 WP_005103244.1 hypothetical protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30119.24 Da        Isoelectric Point: 9.2878

>NTDB_id=599495 ABWED_RS12555 WP_005103248.1 2593648..2594448(-) (pilF) [Acinetobacter lwoffii strain ED23-35]
MRNLTSKFALISTVCVSLVLVACQTPDLGQKDPEKATKVRTQLAVEYLRTGDLDAAKRALDQALESSPRDSQANMMMGVL
LQQEGSKLNLEKADHYFKRAISADPKNAQARNNYGTYLYQLERYNDAIEQFQIAGATLGYDQRFRALENMGRIYLKLGDT
ANAEKSFKQALQANRDSYISMLELAEIFYLNQQFPAATQMYQQFVRGVGQKNQGARALWVGIRTARAGGDQLGMQVLVNQ
LRALFPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=599495 ABWED_RS12555 WP_005103248.1 2593648..2594448(-) (pilF) [Acinetobacter lwoffii strain ED23-35]
TTGAGAAATCTAACATCTAAGTTTGCTTTGATTTCAACAGTATGTGTGTCTTTGGTTTTAGTCGCGTGTCAGACGCCAGA
TCTGGGTCAGAAAGACCCTGAAAAGGCCACCAAAGTCCGTACCCAATTGGCGGTAGAATATTTGAGAACAGGTGACCTGG
ATGCAGCTAAACGTGCGCTGGATCAGGCACTTGAAAGCAGTCCGCGTGATTCTCAAGCGAATATGATGATGGGCGTGCTA
TTGCAGCAGGAAGGTAGCAAGCTTAATCTCGAAAAAGCAGATCATTATTTCAAGCGTGCGATTTCGGCAGATCCGAAAAA
TGCACAGGCGCGAAATAATTACGGCACTTATTTATATCAGCTTGAACGCTATAATGATGCGATCGAGCAGTTTCAAATTG
CTGGTGCCACGTTGGGTTATGATCAGCGATTCCGTGCATTGGAAAATATGGGGCGGATTTACCTGAAACTGGGTGATACA
GCAAATGCTGAAAAATCATTCAAACAGGCGCTGCAAGCCAATCGCGATTCTTATATTTCAATGTTAGAGTTGGCAGAAAT
ATTTTATTTGAACCAGCAGTTCCCGGCGGCGACACAAATGTATCAACAGTTTGTGCGTGGTGTGGGACAGAAAAATCAGG
GTGCCCGTGCGCTCTGGGTCGGCATTCGCACTGCGCGTGCCGGGGGTGATCAGTTGGGTATGCAGGTATTGGTCAACCAG
TTACGTGCACTTTTTCCTGAAAGCCAGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(42-81)

Tetratricopeptide repeat (TPR_17)

(97-129)

Tetratricopeptide repeat (TPR_8)

(145-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9QCA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78.462

97.744

0.767
  pilF Acinetobacter baylyi ADP1

69.549

100

0.695