Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NBY14_RS22605 Genome accession   NZ_CP098217
Coordinates   4725215..4726711 (-) Length   498 a.a.
NCBI ID   WP_001551783.1    Uniprot ID   -
Organism   Escherichia coli strain Z0117EC0032     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4720215..4731711
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBY14_RS22580 (NBY14_22580) panM 4720437..4720820 (+) 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
  NBY14_RS22585 (NBY14_22585) livJ 4721020..4722123 (-) 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  NBY14_RS22590 (NBY14_22590) rpoH 4722394..4723248 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  NBY14_RS22595 (NBY14_22595) ftsX 4723493..4724551 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  NBY14_RS22600 (NBY14_22600) ftsE 4724544..4725212 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  NBY14_RS22605 (NBY14_22605) pilA 4725215..4726711 (-) 1497 WP_001551783.1 signal recognition particle-docking protein FtsY Machinery gene
  NBY14_RS22610 (NBY14_22610) rsmD 4726861..4727457 (+) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  NBY14_RS22615 (NBY14_22615) yhhL 4727447..4727716 (+) 270 WP_001295207.1 DUF1145 family protein -
  NBY14_RS22620 (NBY14_22620) yhhM 4727719..4728078 (-) 360 WP_000042886.1 DUF2500 domain-containing protein -
  NBY14_RS22625 (NBY14_22625) yhhN 4728219..4728845 (+) 627 WP_000964718.1 lysoplasmalogenase -
  NBY14_RS22630 (NBY14_22630) zntA 4728919..4731117 (+) 2199 WP_001551784.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  NBY14_RS22635 (NBY14_22635) tusA 4731219..4731464 (-) 246 WP_000130615.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54701.53 Da        Isoelectric Point: 4.1660

>NTDB_id=598376 NBY14_RS22605 WP_001551783.1 4725215..4726711(-) (pilA) [Escherichia coli strain Z0117EC0032]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPAKASEQAVEEQPQAHTEAEAETFAAEVVEVTEQVAE
SEKAQPEAEVVAQPELVAEETPEPVAIEREELPLPEDVNAEEVSPEEWRAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=598376 NBY14_RS22605 WP_001551783.1 4725215..4726711(-) (pilA) [Escherichia coli strain Z0117EC0032]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGCGAAGGCTTCGGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAGGCCGAAGCGGAAACTTTTGCTGCCGAAGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACTGGTCGCAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCGGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCAGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTAGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACTAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATAATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTAGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATTCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

49.864

74.096

0.369