Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NBY18_RS19920 Genome accession   NZ_CP098206
Coordinates   4063169..4063609 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain Z0117EC0054     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4058169..4068609
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBY18_RS19900 (NBY18_19890) aroP 4059169..4060539 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  NBY18_RS19905 (NBY18_19895) ampE 4060582..4061436 (-) 855 WP_000171983.1 beta-lactamase regulator AmpE -
  NBY18_RS19910 (NBY18_19900) ampD 4061433..4061984 (-) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NBY18_RS19915 (NBY18_19905) nadC 4062072..4062965 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NBY18_RS19920 (NBY18_19910) pilA 4063169..4063609 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  NBY18_RS19925 (NBY18_19915) pilB 4063619..4065004 (+) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  NBY18_RS19930 (NBY18_19920) hofC 4064994..4066196 (+) 1203 WP_000157254.1 protein transport protein HofC -
  NBY18_RS19935 (NBY18_19925) guaC 4066231..4067274 (-) 1044 WP_001217338.1 GMP reductase -
  NBY18_RS19940 (NBY18_19930) - 4067430..4067474 (-) 45 WP_120795372.1 protein YacM -
  NBY18_RS19945 (NBY18_19935) coaE 4067499..4068119 (+) 621 WP_001269535.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=598024 NBY18_RS19920 WP_000360914.1 4063169..4063609(+) (pilA) [Escherichia coli strain Z0117EC0054]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=598024 NBY18_RS19920 WP_000360914.1 4063169..4063609(+) (pilA) [Escherichia coli strain Z0117EC0054]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363