Detailed information NTG0597957

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: K6T24_RS05600
• Genome accession: NZ_CP081872
• Coordinates: 1098030..1101755 (+)
• Length: 1241 a.a.
• NCBI ID: WP_085450062.1
• Uniprot ID: -
• Organism: Bacillus toyonensis strain UTDF19-29B
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1093030..1106755

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K6T24_RS05585 (K6T24_05570)	-	1093062..1093655 (+)	594	WP_000347508.1	TVP38/TMEM64 family protein	-
K6T24_RS05590 (K6T24_05575)	lepB	1093838..1094401 (+)	564	WP_000751916.1	signal peptidase I	-
K6T24_RS05595 (K6T24_05580)	addB	1094518..1098033 (+)	3516	WP_000058644.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
K6T24_RS05600 (K6T24_05585)	addA	1098030..1101755 (+)	3726	WP_085450062.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
K6T24_RS05605 (K6T24_05590)	-	1101779..1102057 (+)	279	WP_085450061.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
K6T24_RS05610 (K6T24_05595)	gerPF	1102146..1102361 (-)	216	WP_001141566.1	spore germination protein GerPF	-
K6T24_RS05615 (K6T24_05600)	-	1102404..1102790 (-)	387	WP_000902325.1	spore germination protein GerPE	-
K6T24_RS05620 (K6T24_05605)	gerPD	1102806..1103000 (-)	195	WP_001052804.1	spore germination protein GerPD	-
K6T24_RS05625 (K6T24_05610)	gerPC	1103007..1103621 (-)	615	WP_001070753.1	spore germination protein GerPC	-
K6T24_RS05630 (K6T24_05615)	gerPB	1103690..1103896 (-)	207	WP_001012514.1	spore germination protein GerPB	-
K6T24_RS05635 (K6T24_05620)	gerPA	1103911..1104132 (-)	222	WP_001111187.1	spore germination protein GerPA	-
K6T24_RS05640 (K6T24_05625)	-	1104229..1104408 (-)	180	WP_000462852.1	aspartyl-phosphate phosphatase Spo0E family protein	-
K6T24_RS05645 (K6T24_05630)	-	1104656..1105555 (+)	900	WP_001213082.1	fumarylacetoacetate hydrolase family protein	-
K6T24_RS05650 (K6T24_05635)	-	1105591..1105872 (-)	282	WP_000926856.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142808.96 Da
• Isoelectric Point: 4.8054

>NTDB_id=597957 K6T24_RS05600 WP_085450062.1 1098030..1101755(+) (addA) [Bacillus toyonensis strain UTDF19-29B]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERMIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVRFQLETAEQHI
RKATELAMLPDGPAPRVETLQADAALLGMLLSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDIVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGSGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVLEGEEGTEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKDAPLEEEQELHEKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLESERKEEVYDRLLWKYGYEEATSHRAKQSVTEIKRNYQSEDGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITAETLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKNGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVKIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=597957 K6T24_RS05600 WP_085450062.1 1098030..1101755(+) (addA) [Bacillus toyonensis strain UTDF19-29B]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCCGTTGTAGCGAATGGACG
TGACATTTTAGTCGCAGCAGCAGCTGGATCGGGAAAAACAGCAGTATTAGTTGAACGTATGATTAAAAAGATTATTAACG
AGGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAATCGAATT
GGAGAAGCGTTAGAAAAAGTACTAATTGATGAGCCAGGATCTCAGCATGTAAGAAAACAGCTGAGCCTATTAAATAAAGC
ATCTATTTCAACGATCCATTCATTTTGTTTACAAGTAATTAGAGGGTATTACTACATGCTTGATGTTGATCCTCGTTTTC
GTATTGCAAATCAAACAGAAAATGAGTTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGAATCGAA
GATAATAGTATTTTCTTTGAATTAGTTGATCGCTATACGAGTGACCGTAGTGATGATGACTTACAAAGAATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATACGACGTGGAAGGAA
AGACAATTGAAGATTTAGTGTATGCTTCTTACTTATTAGAAGATGTGAGATTCCAGCTTGAAACAGCGGAACAACATATT
CGTAAAGCAACCGAACTCGCAATGCTTCCTGACGGCCCGGCGCCTCGCGTTGAAACCCTGCAAGCGGATGCAGCTTTACT
TGGAATGTTATTATCAGCAGCTCGTGAATCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGCATTAAGAAAAGTGATTATAATGAAGATATTGTAAAACAAGTCGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGCTATTTAGCCGCAGACCAGAAAGCTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTACAGCTCGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGCGAGCAAAGTGAAGACGGTGAAATGAAGCCGTCCGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCTGAGGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAGGAATCGATTATTAAATTTGT
AACGAAAGATTCTGAGAGTGAAGGGAACTTGTTCATGGTTGGTGACGTAAAACAGTCAATCTACCGCTTCCGACTAGCAG
AACCAGGCTTATTCTTAGGAAAGTATAAACGCTTCACGCAAGAAGGATCGGGCGGCGGAATGAAGATTGATTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTACTAGCAGGTACGAACTTTATTTTTAAACAAATTATGGGCGAAGAAGTTGGAGAGAT
TGACTACGACGCTGACGCTGAATTAAAACTAGGCGCTAGTTATCCAGAAGGTGAAGATGTAGCGGCTGAATTATTATGCA
TTCAGCAAACGGAAGAAGAAGTGCTAGAGGGTGAAGAAGGTACGGAAGTCGAAAAGGCGCAACTGGAAGCTCGTCTTATG
GCGCAGCGCATTAAAGCGATGGTCGATTCAGGTTATGAAGTGTATGACCGAAAAACGGATAGTATGCGACCAGTGCAATA
TCGTGATTTCGTTATTTTACTTCGCTCTATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAACTACAAGGAATTCCAG
TATATGCAGACCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCACTTGCAGCAGTACTTCGTTCACCGATCGTTGGATTAAATGATGAAGAACTTGCGACGCTTCG
TGCTCACGGAAAGAAAGGGTCATTTTATGAAGTAATGAGCTCATTCTTAAAAGATGCACCGCTTGAAGAAGAACAAGAAC
TTCATGAAAAACTAGAATGGTTTTATAACTTACTGCAAGGATGGCGTGAATTCGCGCGCCAACAATCACTTTCTGATTTA
ATTTGGAAAGTGTATGGTGAGACAGGGTATTATGACTTTGTCGGCGGTTTACCAGCTGGAAAGCAAAGGCAGGCAAACTT
GCGCGTATTATATGACCGCGCAAGGCAATATGAAGCAACATCATTTAGAGGATTATTCCGCTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGACGATATGGGGACGGCGAGAGCTCTTGGTGAACAAGAAGATGTTGTTCGCATTATGACGATT
CATAAAAGTAAGGGACTTGAGTTCCCGGTCGTATTTGTAGCTGGACTCGGTCGTCGTTTTAATACACAAGATTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCACAGTTCATTGATCCGCGTAAACGAATTAAATATACGACATTAT
CACAGCTTGCAATTAAACGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTATTATACGTAGCTTTAACGCGT
GCAAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTCGATGCGAGGGAACA
TAGTGAATGGTTATTACCAGATCACATACGTGCTGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ACCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGTATTCCAGATGAAATTTACGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTGGACGGTAACACGCTACTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACTTCG
TGAGAAAAAGGCTGTTCCGTTAGAAAGTGAACGAAAAGAAGAAGTGTACGACAGATTACTGTGGAAGTACGGATATGAGG
AAGCAACCTCTCACCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAACTATCAATCAGAAGATGGTAGTGATAACGCC
TTTATTAAAAAACTACGTGCACCAATTAAAACGCGTCCTCGTTTCATGGAGAAAAAAGGGTTAACGTACGCAGAGCGCGG
AACAGCAGTCCATGCCGTTATGCAGCATGTAGATTTGAAGAAACCGATTACGGCCGAAACGCTACAAGAACAAATCGCAG
GAATGGTAAATAAGGAATTATTAACATTCGAGCAGGCGGAAGAAATAGCCATTGAAAAAGTAATTTCATTCTTTGACAGT
GACTTAGGTAAAAGGGTATTAGCGGCAAAAAGTGTAGAGCGCGAAGTACCATTTACGATGATGCTTGCAGCTGAAGAAGC
GTATCAAGATTGGCAAGGGAAGAACGGCGAATCGATTCTTGTCCAAGGGGTTATTGACTGCATGATTGAAGAGGAAGACG
GCATTACGTTAATCGACTTTAAAACGGATACGATTGAAGGAAAGTTCCCAGGGGGATTCGATCAAGCGAAACCAATTTTA
GAGGATCGATATAAAGTGCAGCTTTCGTTATATGCAAAAGCACTCGAAAAAAGCTTACAACATCCTGTGAAAGAGAAATG
CTTATACTTCTTTGATGGGAATCATGTTGTGAAAATTGAGGAATAG

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.488	100	0.537