Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   K5P74_RS04260 Genome accession   NZ_CP081846
Coordinates   863209..865026 (+) Length   605 a.a.
NCBI ID   WP_010746499.1    Uniprot ID   R2NTZ5
Organism   Enterococcus raffinosus strain CX012922     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 858209..870026
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5P74_RS04235 (K5P74_04235) - 858535..859254 (-) 720 WP_222226584.1 HAD family hydrolase -
  K5P74_RS04240 (K5P74_04240) spxA 860033..860431 (+) 399 WP_010739854.1 transcriptional regulator SpxA -
  K5P74_RS04245 (K5P74_04245) - 860467..861072 (-) 606 WP_010746502.1 HAD family hydrolase -
  K5P74_RS04250 (K5P74_04250) - 861296..861940 (+) 645 WP_010746501.1 adaptor protein MecA -
  K5P74_RS04255 (K5P74_04255) - 862078..863076 (+) 999 WP_010746500.1 competence protein CoiA -
  K5P74_RS04260 (K5P74_04260) pepF 863209..865026 (+) 1818 WP_010746499.1 oligoendopeptidase F Regulator
  K5P74_RS04265 (K5P74_04265) - 865341..866519 (+) 1179 WP_222226585.1 class C sortase -
  K5P74_RS04270 (K5P74_04270) - 866521..867699 (+) 1179 WP_222226586.1 class C sortase -
  K5P74_RS04275 (K5P74_04275) - 867734..868378 (-) 645 WP_010746496.1 DsbA family protein -
  K5P74_RS04280 (K5P74_04280) - 868375..868983 (-) 609 WP_222226587.1 CYTH domain-containing protein -
  K5P74_RS04285 (K5P74_04285) - 869189..869854 (+) 666 WP_010746494.1 GTP pyrophosphokinase family protein -

Sequence

Protein


Download         Length: 605 a.a.        Molecular weight: 69295.85 Da        Isoelectric Point: 4.6163

>NTDB_id=597704 K5P74_RS04260 WP_010746499.1 863209..865026(+) (pepF) [Enterococcus raffinosus strain CX012922]
MSEAKQLPKRQEVPTELTWDLTKIFTDDAAFETKFQALSDELTEVDKIKGSLGKGAAEFLTAIEYVLDIYRKAEVIYVYA
HLKNDEDTANTTNQALYARASSLIAQVSEAVSWFEPEMLELSDQEIWSFFEAEPKLELYRHFVEQIISERAHVLPAEQEA
LLAGASEIFSSSADTFSVMNNADLKFPVVQNEAGEDVQLTHGVYGQLLESTDRKVREQAFKALYSVYHQFRNTFASTLST
HIKGHNYKAKIRGYASARAAALSGNHIPEDVYETLTLVVNQYLPLLHRYVELRKELLGLEELHMYDLYTPLLAEAPVKFT
YEEAQAKALEALKPLGEEYLEVVKKAFDERWIDVVENQGKRSGAYSSGAYDTQPYILLNWHDRLDELYTLVHEMGHSVHS
YFTRHTQPYVYGDYSIFLAEIASTTNENLLTSYLLETEKDPKIRAYIINHFLDGFKGTIFRQTQFAEFEHFIHTEDAAGN
PLTSEHLSKFYGELNAKYYGPIVVNDEEIKDEWSRIPHFYYNYYVYQYATGFSAASALAKRIVEGEPDALEKYLTYLKSG
SSDYPIEVMKKAGVDMTQPTYIEDAMEIFALRLDELEQLVQELKA

Nucleotide


Download         Length: 1818 bp        

>NTDB_id=597704 K5P74_RS04260 WP_010746499.1 863209..865026(+) (pepF) [Enterococcus raffinosus strain CX012922]
ATGAGTGAAGCAAAACAATTGCCAAAACGGCAGGAAGTACCCACTGAACTGACTTGGGACTTAACTAAAATTTTTACAGA
TGACGCCGCCTTTGAAACAAAATTCCAGGCCTTATCTGATGAACTGACTGAAGTGGACAAGATCAAAGGGAGTTTAGGAA
AAGGTGCGGCCGAATTTTTAACGGCGATCGAATATGTCTTAGATATTTATCGTAAGGCGGAAGTCATTTATGTATATGCT
CATTTAAAAAATGATGAAGATACCGCCAATACGACGAACCAAGCATTGTATGCGCGAGCTTCTAGCTTAATTGCACAAGT
GAGTGAAGCGGTTAGCTGGTTTGAGCCAGAAATGCTGGAGCTTTCTGACCAAGAAATCTGGTCATTTTTCGAAGCAGAGC
CCAAATTAGAATTATATCGTCATTTTGTTGAGCAGATCATTTCAGAGCGAGCGCATGTCTTGCCGGCTGAACAGGAAGCC
TTATTAGCAGGAGCCAGTGAAATTTTCAGCAGCTCAGCAGATACTTTTTCTGTCATGAACAACGCCGATCTTAAATTTCC
AGTGGTACAAAATGAAGCAGGCGAAGATGTTCAATTAACACACGGTGTGTATGGTCAATTATTGGAAAGCACGGATCGAA
AGGTGAGAGAACAAGCCTTTAAGGCGCTGTACTCAGTGTACCATCAATTCCGTAACACCTTTGCTTCAACCTTAAGCACG
CATATTAAAGGGCATAATTACAAGGCGAAGATTCGCGGATATGCCTCTGCGCGGGCGGCTGCACTTAGCGGAAATCATAT
CCCGGAAGATGTTTATGAAACATTGACGCTAGTAGTCAACCAATATTTACCTCTATTGCATCGTTATGTGGAATTACGGA
AAGAACTGCTCGGTTTAGAAGAACTGCACATGTATGATTTGTACACACCGCTTTTAGCAGAAGCGCCAGTGAAGTTCACT
TACGAAGAAGCACAGGCAAAAGCGTTAGAAGCATTGAAACCACTAGGTGAGGAATACTTAGAAGTCGTTAAAAAAGCCTT
CGATGAACGCTGGATTGATGTTGTGGAAAATCAAGGGAAGCGCAGCGGGGCCTACTCTTCTGGTGCCTATGACACACAGC
CATATATTTTATTAAACTGGCATGATCGCCTAGATGAATTGTATACATTGGTACATGAGATGGGTCATAGTGTTCATAGC
TATTTCACACGACACACGCAACCTTATGTTTACGGTGATTACTCCATCTTCTTAGCCGAAATTGCTTCAACAACCAATGA
GAATCTTTTGACCAGCTATCTATTGGAAACAGAAAAAGATCCCAAAATTCGGGCCTATATCATTAATCATTTCTTAGATG
GCTTTAAGGGAACGATTTTCCGTCAGACGCAATTCGCGGAGTTTGAGCACTTTATTCATACAGAAGATGCTGCAGGAAAT
CCATTAACTAGCGAACACCTAAGCAAGTTTTACGGGGAACTAAACGCGAAGTATTATGGACCAATCGTAGTGAATGACGA
AGAAATCAAGGATGAATGGTCAAGAATTCCGCATTTCTACTATAATTACTATGTTTACCAATATGCGACTGGTTTTTCTG
CAGCAAGTGCCTTAGCGAAACGAATCGTAGAAGGCGAGCCAGATGCATTAGAAAAATACTTAACCTACTTAAAATCCGGC
AGCAGTGATTACCCAATCGAGGTCATGAAGAAGGCAGGGGTTGATATGACTCAGCCAACCTATATTGAAGATGCGATGGA
AATTTTTGCGCTACGATTAGATGAGCTGGAACAACTTGTCCAAGAACTAAAGGCATAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(207-587)

Oligopeptidase F (Peptidase_M3_N)

(117-186)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R2NTZ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

55.051

98.182

0.541