Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   K5P74_RS00655 Genome accession   NZ_CP081846
Coordinates   127026..128399 (+) Length   457 a.a.
NCBI ID   WP_010747276.1    Uniprot ID   R2NRU4
Organism   Enterococcus raffinosus strain CX012922     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 122026..133399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5P74_RS00635 (K5P74_00635) - 122249..122995 (+) 747 WP_222226237.1 TIGR03915 family putative DNA repair protein -
  K5P74_RS00640 (K5P74_00640) - 123117..124073 (+) 957 WP_222226238.1 cation diffusion facilitator family transporter -
  K5P74_RS00645 (K5P74_00645) - 124498..126297 (+) 1800 WP_222226239.1 glycerophosphoryl diester phosphodiesterase membrane domain-containing protein -
  K5P74_RS00650 (K5P74_00650) - 126489..126995 (+) 507 WP_010747277.1 hypothetical protein -
  K5P74_RS00655 (K5P74_00655) radA 127026..128399 (+) 1374 WP_010747276.1 DNA repair protein RadA Machinery gene
  K5P74_RS00660 (K5P74_00660) - 128477..129631 (+) 1155 WP_010747275.1 PIN/TRAM domain-containing protein -
  K5P74_RS00665 (K5P74_00665) gltX 129729..131186 (+) 1458 WP_222226240.1 glutamate--tRNA ligase -
  K5P74_RS00670 (K5P74_00670) epsC 131806..132339 (+) 534 WP_010747273.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 50036.52 Da        Isoelectric Point: 7.7029

>NTDB_id=597690 K5P74_RS00655 WP_010747276.1 127026..128399(+) (radA) [Enterococcus raffinosus strain CX012922]
MAKKSKTQFICQECGYVSPKYLGRCPNCGNWNTMVEEIEQNHPEDRRNRVSLTGERATPTKLKDVVFKKETRVQTELAEL
NRVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSHQLAATGGTVLYVSGEESAQQIKLRAERLGSSETDFYLYAETDMGEIS
RTIENLKPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKLAKTNGIAIFIVGHVTKEGNIAGPRMLEHMVDTVLYF
EGEKHHSFRILRAVKNRFGSTNEIGIFEMRERGLVEVANPSQVFLEERLEGATGSAIVVAMEGSRPILVEVQALVTPTVF
GNAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAVAVAIASSYKENGTEATECFIGEIGLT
GEIRRVNSIEQRVKEASKLGFKKIYLPKNNMGDWKPPKGIELVPVATLGETLRKVFR

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=597690 K5P74_RS00655 WP_010747276.1 127026..128399(+) (radA) [Enterococcus raffinosus strain CX012922]
TTGGCAAAAAAATCCAAGACACAATTTATCTGCCAAGAATGTGGTTATGTTTCGCCTAAGTATTTAGGGCGTTGTCCTAA
CTGCGGAAATTGGAACACGATGGTAGAGGAGATTGAGCAAAATCATCCCGAAGATCGACGCAACCGTGTCAGTTTAACCG
GAGAGCGTGCGACACCTACCAAATTAAAGGATGTCGTCTTCAAAAAGGAAACGCGCGTTCAGACAGAATTAGCTGAATTG
AATCGCGTTCTTGGCGGCGGAGTGGTTCCAGGTTCATTGGTATTAATCGGTGGGGACCCAGGTATTGGGAAATCCACCTT
ACTATTGCAAGTATCCCATCAATTAGCGGCAACCGGCGGCACAGTTTTGTATGTTTCTGGAGAAGAAAGTGCGCAGCAGA
TAAAATTGCGTGCGGAGCGCCTGGGATCAAGTGAGACAGATTTTTATTTATATGCGGAAACAGACATGGGAGAAATCAGC
CGAACGATTGAAAATCTGAAGCCGGATTATGTGATCATCGACTCGATCCAAACCATGACACAACCAGATATCACTAGTGT
AGCGGGCTCGGTCAGCCAGGTTCGGGAGACCACCGCAGAGCTATTGAAGCTGGCGAAGACGAACGGCATTGCGATTTTTA
TCGTAGGTCATGTGACCAAAGAGGGAAATATCGCAGGTCCGCGAATGCTGGAGCATATGGTGGACACTGTTTTATACTTT
GAAGGCGAGAAGCATCACAGCTTTCGAATCTTACGAGCGGTAAAAAATCGTTTCGGTTCCACTAATGAAATCGGAATTTT
CGAGATGAGAGAACGGGGGCTGGTCGAAGTGGCCAATCCTTCACAGGTTTTCCTAGAGGAACGCCTAGAAGGGGCGACCG
GATCAGCAATCGTGGTTGCCATGGAAGGCAGCCGACCAATTTTGGTTGAGGTGCAGGCATTGGTAACCCCCACTGTTTTC
GGCAACGCGAAGCGCACAACGACCGGCTTAGATTTCAACCGTGTTTCTTTGATCATGGCTGTTTTGGAAAAACGCGCCGG
GTTGTTGCTGCAAAATCAAGACGCCTACTTAAAGGCGGCAGGCGGGGTAAAGTTAAATGAACCGGCCATTGATTTAGCGG
TGGCTGTGGCGATTGCCTCCAGCTACAAAGAAAATGGCACGGAAGCCACCGAATGCTTTATCGGTGAAATTGGTTTGACG
GGTGAAATTCGTCGGGTCAATAGCATTGAGCAGCGGGTGAAAGAAGCCAGCAAATTGGGCTTCAAAAAAATCTATCTGCC
TAAGAACAATATGGGCGATTGGAAACCGCCAAAAGGAATCGAATTGGTTCCTGTGGCAACCTTAGGGGAAACCTTAAGAA
AAGTATTTCGTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(57-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R2NRU4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.123

99.781

0.74
  radA Streptococcus mitis SK321

73.904

99.781

0.737
  radA Streptococcus pneumoniae Rx1

73.904

99.781

0.737
  radA Streptococcus pneumoniae D39

73.904

99.781

0.737
  radA Streptococcus pneumoniae R6

73.904

99.781

0.737
  radA Streptococcus pneumoniae TIGR4

73.904

99.781

0.737
  radA Bacillus subtilis subsp. subtilis str. 168

64.967

98.687

0.641