Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   K5X77_RS05650 Genome accession   NZ_CP081833
Coordinates   1175763..1177133 (-) Length   456 a.a.
NCBI ID   WP_168413290.1    Uniprot ID   A0AAE9XII1
Organism   Vagococcus lutrae strain BN31     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1170763..1182133
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5X77_RS05630 (K5X77_05630) cysS 1170838..1172256 (-) 1419 WP_168413288.1 cysteine--tRNA ligase -
  K5X77_RS05635 (K5X77_05635) epsC 1172240..1172845 (-) 606 WP_272158218.1 serine O-acetyltransferase EpsC -
  K5X77_RS05640 (K5X77_05640) gltX 1173052..1174518 (-) 1467 WP_222316880.1 glutamate--tRNA ligase -
  K5X77_RS05645 (K5X77_05645) - 1174614..1175744 (-) 1131 WP_126762357.1 PIN/TRAM domain-containing protein -
  K5X77_RS05650 (K5X77_05650) radA 1175763..1177133 (-) 1371 WP_168413290.1 DNA repair protein RadA Machinery gene
  K5X77_RS05655 (K5X77_05655) - 1177238..1177771 (-) 534 WP_222316881.1 dUTP diphosphatase -
  K5X77_RS05660 (K5X77_05660) - 1177887..1178144 (-) 258 WP_126762350.1 metal-sensing transcriptional repressor -
  K5X77_RS05665 (K5X77_05665) - 1178159..1179805 (-) 1647 WP_222316882.1 FAD-dependent oxidoreductase -
  K5X77_RS05670 (K5X77_05670) - 1179806..1180120 (-) 315 WP_023607060.1 rhodanese-like domain-containing protein -
  K5X77_RS05675 (K5X77_05675) - 1180234..1180953 (-) 720 WP_222316883.1 pseudouridine synthase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50116.58 Da        Isoelectric Point: 6.1973

>NTDB_id=597656 K5X77_RS05650 WP_168413290.1 1175763..1177133(-) (radA) [Vagococcus lutrae strain BN31]
MAKKQRTQFECQACGYISPKYLGRCPNCGAWNEMMEVKIQEEPDRHSRVSLAGKRTTAKRLTEVAMEKEPRVKTQMDELN
RVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSQQLNQADGVVLYVSGEESAEQIKMRAERLGIKGNDFYIYPETDMSQIRQ
TIEELQPDYVIIDSIQTMMQPEIASASGSVSQVRETTAELMQIAKTNHIAIFIVGHVTKEGALAGPRMLEHMVDTVLYFE
GDRHHTFRILRAVKNRFGSTNEIGIFEMVESGLQEVLNPSEVFLEERLDGATGSAIVVSMEGSRPILAEVQALVSPTLFG
NAKRTATGLDYNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLDEPAIDLAIAMSIASSYKDTGTSATECFIGEIGLTG
EVRRVNRIDQRIREAQKLGFKKVYVPKNNLHGYTPPADIEVVGIATLSEALRKVFP

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=597656 K5X77_RS05650 WP_168413290.1 1175763..1177133(-) (radA) [Vagococcus lutrae strain BN31]
GTGGCGAAAAAGCAACGTACACAATTTGAATGTCAAGCATGCGGTTACATTTCTCCTAAATATTTAGGTAGATGCCCTAA
CTGCGGTGCTTGGAATGAAATGATGGAAGTGAAGATACAAGAAGAACCTGACCGTCATAGTCGCGTCAGCCTAGCTGGCA
AGCGAACAACGGCTAAGCGATTAACAGAAGTGGCGATGGAAAAAGAACCCCGTGTAAAAACACAAATGGACGAGCTTAAT
CGTGTGTTGGGTGGTGGCGTTGTTCCCGGGTCACTTGTTTTAATTGGTGGAGACCCAGGGATTGGGAAATCTACGTTATT
ACTACAAGTTTCGCAACAGCTTAATCAAGCTGATGGCGTAGTTTTATACGTGTCTGGTGAGGAAAGTGCCGAGCAGATAA
AAATGCGTGCGGAGCGTTTAGGGATTAAAGGCAATGATTTTTATATTTATCCAGAAACAGATATGTCTCAGATACGTCAA
ACTATCGAGGAATTACAACCAGACTATGTCATCATTGATTCCATCCAGACGATGATGCAACCAGAGATAGCCAGTGCATC
AGGTAGTGTGAGCCAAGTCCGAGAAACGACTGCTGAGTTGATGCAAATTGCTAAAACTAATCATATTGCTATTTTTATCG
TAGGCCATGTGACAAAGGAAGGCGCCTTAGCAGGTCCTAGAATGTTAGAACATATGGTAGATACGGTGTTGTATTTTGAA
GGGGATCGTCATCATACCTTTCGAATTTTGCGAGCGGTAAAAAACCGTTTTGGATCGACAAATGAGATTGGGATATTTGA
GATGGTGGAGTCGGGCTTACAGGAAGTCTTAAATCCTTCTGAAGTCTTCTTAGAAGAGCGATTAGACGGTGCGACTGGCT
CGGCGATTGTGGTGTCGATGGAAGGCAGTCGTCCGATTTTAGCAGAAGTGCAAGCTCTTGTATCTCCTACTTTATTTGGA
AACGCAAAGCGGACAGCGACTGGGTTAGATTATAACCGGGTGTCACTGATTATGGCAGTGTTAGAAAAACGAGCCGGTCT
TTTGTTGCAAAATCAAGATGCGTATTTAAAAGCTGCTGGTGGTGTTAAGCTAGATGAACCGGCGATTGACTTAGCCATTG
CGATGAGTATCGCTTCGAGCTACAAAGATACGGGAACTAGTGCTACCGAGTGTTTTATTGGCGAAATTGGTTTGACCGGT
GAAGTGCGACGGGTGAATCGGATTGATCAACGCATTAGAGAAGCTCAAAAGTTAGGCTTTAAAAAAGTGTACGTACCAAA
AAACAATTTACATGGATATACACCGCCAGCTGATATTGAAGTGGTTGGAATTGCCACCTTATCAGAAGCGTTAAGAAAAG
TTTTTCCTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-34)

AAA domain (AAA_25)

(71-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.066

99.781

0.739
  radA Streptococcus mitis SK321

74.066

99.781

0.739
  radA Streptococcus pneumoniae Rx1

73.846

99.781

0.737
  radA Streptococcus pneumoniae D39

73.846

99.781

0.737
  radA Streptococcus pneumoniae R6

73.846

99.781

0.737
  radA Streptococcus pneumoniae TIGR4

73.846

99.781

0.737
  radA Bacillus subtilis subsp. subtilis str. 168

66.15

99.123

0.656