Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NBY23_RS02145 Genome accession   NZ_CP098195
Coordinates   443691..445187 (+) Length   498 a.a.
NCBI ID   WP_001040689.1    Uniprot ID   -
Organism   Escherichia coli strain Z0117EC0098     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 438691..450187
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBY23_RS02115 (NBY23_02110) tusA 438938..439183 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  NBY23_RS02120 (NBY23_02115) zntA 439285..441483 (-) 2199 WP_000106510.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  NBY23_RS02125 (NBY23_02120) yhhN 441557..442183 (-) 627 WP_000964718.1 lysoplasmalogenase -
  NBY23_RS02130 (NBY23_02125) yhhM 442324..442683 (+) 360 WP_000042873.1 DUF2500 domain-containing protein -
  NBY23_RS02135 (NBY23_02130) yhhL 442686..442955 (-) 270 WP_001295207.1 DUF1145 family protein -
  NBY23_RS02140 (NBY23_02135) rsmD 442945..443541 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  NBY23_RS02145 (NBY23_02140) pilA 443691..445187 (+) 1497 WP_001040689.1 signal recognition particle-docking protein FtsY Machinery gene
  NBY23_RS02150 (NBY23_02145) ftsE 445190..445858 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  NBY23_RS02155 (NBY23_02150) ftsX 445851..446909 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  NBY23_RS02160 (NBY23_02155) rpoH 447154..448008 (+) 855 WP_063100092.1 RNA polymerase sigma factor RpoH -
  NBY23_RS02165 (NBY23_02160) livJ 448279..449382 (+) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  NBY23_RS02170 (NBY23_02165) ataR 449532..449798 (+) 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54685.47 Da        Isoelectric Point: 4.1468

>NTDB_id=597638 NBY23_RS02145 WP_001040689.1 443691..445187(+) (pilA) [Escherichia coli strain Z0117EC0098]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVIVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=597638 NBY23_RS02145 WP_001040689.1 443691..445187(+) (pilA) [Escherichia coli strain Z0117EC0098]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAAACGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCTGAACAAGCCGTCGAAG
AGCAGCCGCAGGCGCATACTGAGGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAACCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAAGCGGTGATAGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTTGAAGAAATTGCCCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATCATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
CCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTAGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGGCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCTGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378