Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NBY24_RS19730 Genome accession   NZ_CP098192
Coordinates   4065348..4065788 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain Z0117EC0116     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4060348..4070788
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBY24_RS19710 (NBY24_19715) aroP 4061346..4062719 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  NBY24_RS19715 (NBY24_19720) ampE 4062762..4063616 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  NBY24_RS19720 (NBY24_19725) ampD 4063613..4064164 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NBY24_RS19725 (NBY24_19730) nadC 4064252..4065145 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NBY24_RS19730 (NBY24_19735) pilA 4065348..4065788 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  NBY24_RS19735 (NBY24_19740) pilB 4065798..4067183 (+) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  NBY24_RS19740 (NBY24_19745) hofC 4067173..4068375 (+) 1203 WP_000157227.1 protein transport protein HofC -
  NBY24_RS19745 (NBY24_19750) guaC 4068410..4069453 (-) 1044 WP_001217342.1 GMP reductase -
  NBY24_RS19750 (NBY24_19755) - 4069609..4069653 (-) 45 WP_120795372.1 protein YacM -
  NBY24_RS19755 (NBY24_19760) coaE 4069678..4070298 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=597610 NBY24_RS19730 WP_000360895.1 4065348..4065788(+) (pilA) [Escherichia coli strain Z0117EC0116]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=597610 NBY24_RS19730 WP_000360895.1 4065348..4065788(+) (pilA) [Escherichia coli strain Z0117EC0116]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363