Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NBY25_RS04835 Genome accession   NZ_CP098187
Coordinates   1010693..1011133 (-) Length   146 a.a.
NCBI ID   WP_000360913.1    Uniprot ID   -
Organism   Escherichia coli strain Z0117EC0117     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1005693..1016133
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBY25_RS04810 (NBY25_04810) coaE 1006183..1006803 (-) 621 WP_001269524.1 dephospho-CoA kinase -
  NBY25_RS04815 (NBY25_04815) - 1006828..1006872 (+) 45 WP_120795372.1 protein YacM -
  NBY25_RS04820 (NBY25_04820) guaC 1007028..1008071 (+) 1044 WP_001217338.1 GMP reductase -
  NBY25_RS04825 (NBY25_04825) hofC 1008106..1009308 (-) 1203 WP_000157243.1 protein transport protein HofC -
  NBY25_RS04830 (NBY25_04830) pilB 1009298..1010683 (-) 1386 WP_001535814.1 type II secretion system protein GspE Machinery gene
  NBY25_RS04835 (NBY25_04835) pilA 1010693..1011133 (-) 441 WP_000360913.1 prepilin peptidase-dependent pilin Machinery gene
  NBY25_RS04840 (NBY25_04840) nadC 1011337..1012230 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NBY25_RS04845 (NBY25_04845) ampD 1012318..1012869 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NBY25_RS04850 (NBY25_04850) ampE 1012866..1013720 (+) 855 WP_000171984.1 beta-lactamase regulator AmpE -
  NBY25_RS04855 (NBY25_04855) aroP 1013763..1015133 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15634.80 Da        Isoelectric Point: 4.5237

>NTDB_id=597484 NBY25_RS04835 WP_000360913.1 1010693..1011133(-) (pilA) [Escherichia coli strain Z0117EC0117]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSIVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=597484 NBY25_RS04835 WP_000360913.1 1010693..1011133(-) (pilA) [Escherichia coli strain Z0117EC0117]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCATCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363