Detailed information    

insolico Bioinformatically predicted

Overview

Name   recP/tkt   Type   Machinery gene
Locus tag   NBY25_RS00470 Genome accession   NZ_CP098187
Coordinates   94535..96532 (+) Length   665 a.a.
NCBI ID   WP_001088081.1    Uniprot ID   A0A0H2Z511
Organism   Escherichia coli strain Z0117EC0117     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 89535..101532
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBY25_RS00445 (NBY25_00445) - 90185..90430 (-) 246 WP_001298603.1 hypothetical protein -
  NBY25_RS00450 (NBY25_00450) - 90505..90762 (-) 258 WP_000156431.1 hypothetical protein -
  NBY25_RS00455 (NBY25_00455) tssD 90759..91892 (-) 1134 Protein_80 type VI secretion system tube protein TssD -
  NBY25_RS00460 (NBY25_00460) hxlB 92377..92934 (+) 558 WP_000339041.1 6-phospho-3-hexuloisomerase -
  NBY25_RS00465 (NBY25_00465) ptsG 92976..94484 (+) 1509 WP_000037973.1 glucose-specific PTS transporter subunit IIBC -
  NBY25_RS00470 (NBY25_00470) recP/tkt 94535..96532 (+) 1998 WP_001088081.1 transketolase Machinery gene
  NBY25_RS00475 (NBY25_00475) - 96562..97407 (-) 846 WP_001060027.1 MurR/RpiR family transcriptional regulator -
  NBY25_RS00480 (NBY25_00480) - 97608..98513 (-) 906 WP_000470719.1 LysR substrate-binding domain-containing protein -
  NBY25_RS00485 (NBY25_00485) - 98591..99814 (-) 1224 WP_001227364.1 cytosine permease -
  NBY25_RS00490 (NBY25_00490) - 99840..100229 (-) 390 WP_000214035.1 glutamyl-tRNA amidotransferase -
  NBY25_RS00495 (NBY25_00495) arcC 100246..101202 (-) 957 WP_000240547.1 carbamate kinase -

Sequence

Protein


Download         Length: 665 a.a.        Molecular weight: 72974.63 Da        Isoelectric Point: 5.7465

>NTDB_id=597466 NBY25_RS00470 WP_001088081.1 94535..96532(+) (recP/tkt) [Escherichia coli strain Z0117EC0117]
MNSQLLANAIRMLSVDAIQKANSGHPGAPMGMADIAEVVWRRHLRHNPKNPQWFNRDRYIQSNGHGSMLIYALLHLTGYD
LSMDDIRDFRQLHSRTPGHPEYGYTPGVETTTGPLGQGVANAVGMAIAEKALAAEFNKPGFNIVDHHTWLFLGDGCLMEG
ISHEACGLAGTLKLGNLIAIWDDNGISIDGHVEGWFAEDTAARFRAYGWHVIEGVDGHDPEEVDAAVREAKSVTDKPSLL
CCKTIIGFGSPNKANSHDCHGSALGADEVALVRERLQWPYAPFEIPGEIYAAWDATEKGAQVQQEWDALFADYAKQWPEL
AAEFTRRMKGDLPAGWAENMQKYVHDLQSHPAALATRQVSQKCLNHFADMLPELMGGSADLSPSNLTRHQKSVDFTGENP
AGNYISYGVREFGMSAIMNGLALHGGFIPYGGTFLMFMEYARNALRMAALMKIRSVFVYTHDTIGLGEDGPTHQPVEQLA
SLRLTPNMETWRGCDQVEVAVAWQQAIERKDGPTSLVLTRQPLAQQPRTAAQLAEIARGGYVLSDCDGQPEMILISAGSE
IELVVSAAKALTEEGRKVRVVSMPCTERFDNQDAAYKESVLPKAVRKRLAVEASIAGFWERYVGLDGKVIGMTSFGESAP
ANVLFKHFGFTPENVLAQARELLNS

Nucleotide


Download         Length: 1998 bp        

>NTDB_id=597466 NBY25_RS00470 WP_001088081.1 94535..96532(+) (recP/tkt) [Escherichia coli strain Z0117EC0117]
ATGAATTCACAATTACTGGCTAACGCCATCCGAATGTTAAGTGTTGATGCGATCCAGAAAGCGAACTCCGGTCATCCGGG
CGCGCCGATGGGGATGGCGGATATTGCCGAAGTGGTCTGGCGTCGTCACCTGCGTCATAACCCAAAAAATCCGCAGTGGT
TTAACCGCGACCGCTATATTCAGTCGAACGGGCACGGTTCCATGTTGATTTACGCTTTGCTGCATCTTACAGGCTACGAC
CTGTCGATGGATGATATTCGCGACTTTCGTCAGCTCCACTCCCGCACGCCAGGTCATCCAGAATATGGTTATACGCCGGG
TGTTGAAACCACCACCGGCCCGTTGGGGCAGGGCGTAGCTAATGCGGTGGGGATGGCGATTGCGGAGAAGGCGCTGGCGG
CAGAATTTAACAAACCGGGCTTTAATATTGTTGATCATCACACATGGCTATTTTTGGGTGATGGCTGCCTGATGGAAGGG
ATCTCCCACGAGGCCTGTGGTCTTGCAGGAACGCTGAAGCTCGGCAATCTGATTGCTATCTGGGACGATAACGGTATTTC
GATCGATGGTCACGTAGAAGGCTGGTTTGCTGAAGATACGGCTGCCCGTTTTCGTGCCTACGGCTGGCATGTCATTGAAG
GCGTTGATGGTCACGATCCAGAAGAGGTAGATGCTGCGGTGCGTGAAGCGAAAAGCGTCACGGATAAGCCCAGTCTGCTG
TGCTGCAAAACGATTATCGGTTTTGGTTCGCCAAATAAAGCCAACAGCCACGATTGCCACGGCTCGGCGCTGGGTGCAGA
TGAAGTGGCGCTGGTGCGTGAACGTCTGCAATGGCCGTATGCCCCTTTCGAAATTCCTGGTGAAATTTATGCCGCATGGG
ATGCGACCGAGAAAGGCGCGCAGGTTCAACAGGAATGGGATGCACTGTTTGCTGACTACGCGAAGCAGTGGCCGGAACTG
GCGGCGGAGTTTACTCGTCGCATGAAGGGTGATTTGCCTGCTGGCTGGGCCGAGAATATGCAGAAATATGTGCACGATCT
GCAATCCCATCCGGCGGCGCTGGCAACCCGTCAGGTCAGCCAGAAATGCCTCAATCACTTCGCGGATATGCTGCCGGAAC
TGATGGGCGGCTCGGCGGACTTGTCGCCCTCTAACCTGACTCGCCATCAGAAATCGGTTGATTTCACCGGGGAAAATCCG
GCGGGGAATTACATCTCCTATGGCGTGCGTGAATTTGGTATGTCGGCCATCATGAACGGTCTTGCGCTGCATGGTGGGTT
TATCCCTTACGGCGGTACTTTCCTGATGTTTATGGAATATGCGCGTAATGCACTGCGCATGGCGGCGCTGATGAAGATCC
GCTCGGTGTTTGTTTATACCCACGATACCATCGGCCTGGGCGAAGACGGCCCGACACATCAGCCCGTTGAGCAACTGGCG
TCGCTGCGTCTGACGCCGAATATGGAAACCTGGCGCGGTTGCGATCAGGTGGAAGTGGCGGTAGCCTGGCAACAGGCGAT
TGAGCGCAAAGATGGCCCAACGTCGTTGGTATTAACGCGCCAACCTCTGGCGCAACAGCCGCGCACCGCTGCACAGTTGG
CAGAGATTGCGCGTGGTGGTTATGTACTGAGTGATTGTGACGGTCAGCCTGAGATGATCCTCATTTCTGCGGGTTCAGAG
ATTGAGTTAGTGGTCAGCGCCGCAAAAGCGTTAACGGAAGAAGGGCGTAAAGTTCGCGTGGTTTCCATGCCGTGTACCGA
GCGTTTCGATAATCAGGATGCGGCGTACAAAGAGTCGGTATTGCCAAAAGCTGTACGCAAACGTCTGGCGGTAGAAGCCA
GCATCGCCGGATTCTGGGAACGCTACGTCGGCCTGGACGGTAAAGTGATCGGCATGACGAGCTTTGGTGAGTCTGCTCCG
GCGAATGTGTTGTTCAAACACTTTGGTTTTACGCCGGAAAATGTCCTGGCGCAGGCGCGTGAGCTACTGAACAGCTAA

Domains

Predicted by InterProScan.

Transketolase, pyrimidine binding domain (Transket_pyr)

(354-524)

Transketolase, C-terminal domain (Transketolase_C)

(540-655)

Transketolase, thiamine diphosphate binding domain (Transketolase_N)

(4-333)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z511

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recP/tkt Streptococcus pneumoniae TIGR4

47.041

99.098

0.466