Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NBY27_RS03790 Genome accession   NZ_CP098183
Coordinates   815581..816021 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain Z0117EC0133     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 810581..821021
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBY27_RS03765 (NBY27_03765) coaE 811071..811691 (-) 621 WP_001269527.1 dephospho-CoA kinase -
  NBY27_RS03770 (NBY27_03770) - 811716..811760 (+) 45 WP_120795372.1 protein YacM -
  NBY27_RS03775 (NBY27_03775) guaC 811916..812959 (+) 1044 WP_001217338.1 GMP reductase -
  NBY27_RS03780 (NBY27_03780) hofC 812994..814196 (-) 1203 WP_000157235.1 protein transport protein HofC -
  NBY27_RS03785 (NBY27_03785) pilB 814186..815571 (-) 1386 WP_001025150.1 type II secretion system protein GspE Machinery gene
  NBY27_RS03790 (NBY27_03790) pilA 815581..816021 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  NBY27_RS03795 (NBY27_03795) nadC 816224..817117 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NBY27_RS03800 (NBY27_03800) ampD 817205..817756 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NBY27_RS03805 (NBY27_03805) ampE 817753..818607 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  NBY27_RS03810 (NBY27_03810) aroP 818650..820023 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=597401 NBY27_RS03790 WP_000360904.1 815581..816021(-) (pilA) [Escherichia coli strain Z0117EC0133]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=597401 NBY27_RS03790 WP_000360904.1 815581..816021(-) (pilA) [Escherichia coli strain Z0117EC0133]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363