Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NBY28_RS04035 Genome accession   NZ_CP098178
Coordinates   856403..856843 (-) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain Z0117EC0148     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 851403..861843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBY28_RS04010 (NBY28_04010) coaE 851893..852513 (-) 621 WP_001269527.1 dephospho-CoA kinase -
  NBY28_RS04015 (NBY28_04015) - 852538..852582 (+) 45 WP_120795372.1 protein YacM -
  NBY28_RS04020 (NBY28_04020) guaC 852738..853781 (+) 1044 WP_001217338.1 GMP reductase -
  NBY28_RS04025 (NBY28_04025) hofC 853816..855018 (-) 1203 WP_000157234.1 protein transport protein HofC -
  NBY28_RS04030 (NBY28_04030) pilB 855008..856393 (-) 1386 WP_001470984.1 type II secretion system protein GspE Machinery gene
  NBY28_RS04035 (NBY28_04035) pilA 856403..856843 (-) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  NBY28_RS04040 (NBY28_04040) nadC 857046..857939 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NBY28_RS04045 (NBY28_04045) ampD 858027..858578 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NBY28_RS04050 (NBY28_04050) ampE 858575..859429 (+) 855 WP_000172006.1 beta-lactamase regulator AmpE -
  NBY28_RS04055 (NBY28_04055) aroP 859472..860845 (-) 1374 WP_000969914.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=597323 NBY28_RS04035 WP_000360895.1 856403..856843(-) (pilA) [Escherichia coli strain Z0117EC0148]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=597323 NBY28_RS04035 WP_000360895.1 856403..856843(-) (pilA) [Escherichia coli strain Z0117EC0148]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363