Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NAG72_RS21590 Genome accession   NZ_CP097884
Coordinates   4425257..4425697 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli str. K-12 substr. MG1655 strain K-12     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4420257..4430697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NAG72_RS21570 aroP 4421255..4422628 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  NAG72_RS21575 ampE 4422671..4423525 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  NAG72_RS21580 ampD 4423522..4424073 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NAG72_RS21585 nadC 4424161..4425054 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NAG72_RS21590 pilA 4425257..4425697 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  NAG72_RS21595 pilB 4425707..4427092 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  NAG72_RS21600 hofC 4427082..4428284 (+) 1203 WP_000157266.1 protein transport protein HofC -
  NAG72_RS21605 guaC 4428319..4429362 (-) 1044 WP_001217338.1 GMP reductase -
  NAG72_RS21610 - 4429518..4429562 (-) 45 WP_120795372.1 protein YacM -
  NAG72_RS21615 coaE 4429587..4430207 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=597152 NAG72_RS21590 WP_000360904.1 4425257..4425697(+) (pilA) [Escherichia coli str. K-12 substr. MG1655 strain K-12]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=597152 NAG72_RS21590 WP_000360904.1 4425257..4425697(+) (pilA) [Escherichia coli str. K-12 substr. MG1655 strain K-12]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363