Detailed information    

insolico Bioinformatically predicted

Overview


Name   amiF   Type   Regulator
Locus tag   K5J94_RS03365 Genome accession   NZ_CP081502
Coordinates   676586..677518 (+) Length   310 a.a.
NCBI ID   WP_159565000.1    Uniprot ID   -
Organism   Streptococcus halichoeri strain Shali_VAS-CPH     
Function   internalize XIP (predicted from homology)   
Competence regulation

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 620893..678299 676586..677518 within 0


Gene organization within MGE regions


Location: 620893..678299
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5J94_RS03085 - 620993..621256 (+) 264 WP_002311901.1 peptide-binding protein -
  K5J94_RS03090 - 621277..621360 (+) 84 WP_001814874.1 23S rRNA methyltransferase attenuation leader peptide -
  K5J94_RS03095 erm(B) 621485..622222 (+) 738 WP_010725603.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
  K5J94_RS03100 - 622392..622652 (+) 261 Protein_560 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
  K5J94_RS03105 - 622815..623480 (+) 666 Protein_561 IS1182-like element IS1182 family transposase -
  K5J94_RS03110 - 623516..623761 (+) 246 WP_000567888.1 single-stranded DNA-binding protein -
  K5J94_RS03115 - 623864..624733 (+) 870 WP_000228166.1 nucleotidyltransferase domain-containing protein -
  K5J94_RS03120 - 624714..625448 (+) 735 WP_000662263.1 bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG -
  K5J94_RS03125 - 625481..626344 (+) 864 WP_002294505.1 aminoglycoside nucleotidyltransferase ANT(6)-Ia -
  K5J94_RS03130 - 626388..626915 (+) 528 WP_002294507.1 phosphoribosyltransferase family protein -
  K5J94_RS03135 - 627047..627856 (+) 810 WP_002294509.1 ANT(9) family aminoglycoside nucleotidyltransferase Spw -
  K5J94_RS03140 - 628035..628325 (+) 291 WP_010733534.1 hypothetical protein -
  K5J94_RS03145 - 628376..628834 (-) 459 WP_002325145.1 hypothetical protein -
  K5J94_RS03150 - 628902..629480 (+) 579 WP_088186203.1 recombinase zinc beta ribbon domain-containing protein -
  K5J94_RS03155 lsa(E) 629923..631407 (+) 1485 WP_002294513.1 ABC-F type ribosomal protection protein Lsa(E) -
  K5J94_RS03160 lnu(B) 631461..632264 (+) 804 WP_002294514.1 lincosamide nucleotidyltransferase Lnu(B) -
  K5J94_RS03165 - 633045..633773 (+) 729 Protein_573 recombinase zinc beta ribbon domain-containing protein -
  K5J94_RS03170 - 633916..634140 (+) 225 WP_000205227.1 helix-turn-helix transcriptional regulator -
  K5J94_RS03175 - 634155..634550 (+) 396 Protein_575 nucleotidyltransferase domain-containing protein -
  K5J94_RS03180 - 634625..635305 (+) 681 WP_001015311.1 IS6-like element IS1216 family transposase -
  K5J94_RS03185 - 635478..636830 (+) 1353 WP_074626891.1 ISLre2 family transposase -
  K5J94_RS03190 rpmH 637257..637391 (+) 135 WP_000831903.1 50S ribosomal protein L34 -
  K5J94_RS03195 - 637960..638403 (-) 444 WP_159543286.1 MarR family winged helix-turn-helix transcriptional regulator -
  K5J94_RS03200 - 638548..640191 (+) 1644 WP_159564408.1 alpha-keto acid decarboxylase family protein -
  K5J94_RS09495 - 640548..642200 (-) 1653 WP_231700371.1 helix-turn-helix domain-containing protein -
  K5J94_RS03215 argF 642320..643258 (+) 939 WP_159543282.1 ornithine carbamoyltransferase -
  K5J94_RS03220 - 643302..644807 (+) 1506 WP_159543280.1 YfcC family protein -
  K5J94_RS03225 - 644820..645680 (+) 861 WP_159543496.1 dimethylarginine dimethylaminohydrolase family protein -
  K5J94_RS03230 - 645698..646678 (+) 981 WP_159564406.1 NAD(P)-dependent oxidoreductase -
  K5J94_RS03235 - 646920..647693 (+) 774 WP_159548592.1 TatD family hydrolase -
  K5J94_RS03240 rnmV 647686..648255 (+) 570 WP_221913502.1 ribonuclease M5 -
  K5J94_RS03245 rsmA 648535..649407 (+) 873 WP_159543274.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
  K5J94_RS09605 - 649700..651196 (+) 1497 WP_269437387.1 LPXTG cell wall anchor domain-containing protein -
  K5J94_RS03255 - 651319..652038 (-) 720 WP_221913504.1 DUF4811 domain-containing protein -
  K5J94_RS03260 - 652040..653539 (-) 1500 WP_221913505.1 MDR family MFS transporter -
  K5J94_RS03265 - 653745..654140 (+) 396 WP_221913507.1 hypothetical protein -
  K5J94_RS03270 rsgA 654357..655229 (+) 873 WP_221913509.1 ribosome small subunit-dependent GTPase A -
  K5J94_RS03275 rpe 655238..655900 (+) 663 WP_159548529.1 ribulose-phosphate 3-epimerase -
  K5J94_RS03280 - 655893..656525 (+) 633 WP_221913511.1 thiamine diphosphokinase -
  K5J94_RS03285 - 656593..657798 (+) 1206 WP_408022839.1 DNA recombination protein RmuC -
  K5J94_RS03290 - 657788..658726 (+) 939 WP_221913515.1 3'-5' exoribonuclease YhaM family protein -
  K5J94_RS03295 purR 658837..659649 (+) 813 WP_159564384.1 pur operon repressor -
  K5J94_RS03300 - 659673..662327 (+) 2655 WP_221913517.1 SEC10/PgrA surface exclusion domain-containing protein -
  K5J94_RS03305 rpsL 662500..662913 (+) 414 WP_159543253.1 30S ribosomal protein S12 -
  K5J94_RS03310 rpsG 662934..663404 (+) 471 WP_159543251.1 30S ribosomal protein S7 -
  K5J94_RS03315 fusA 663792..665870 (+) 2079 WP_159543249.1 elongation factor G -
  K5J94_RS03320 gap 666149..667162 (+) 1014 WP_159543247.1 type I glyceraldehyde-3-phosphate dehydrogenase -
  K5J94_RS03325 - 667735..668931 (+) 1197 WP_159564984.1 phosphoglycerate kinase -
  K5J94_RS03330 - 669260..669772 (+) 513 WP_159564986.1 aromatic acid exporter family protein -
  K5J94_RS03335 - 669850..670221 (+) 372 WP_159548541.1 MerR family transcriptional regulator -
  K5J94_RS03340 - 670257..671603 (+) 1347 WP_221913519.1 glutamine synthetase family protein -
  K5J94_RS03345 - 671838..673493 (+) 1656 WP_159543238.1 peptide ABC transporter substrate-binding protein -
  K5J94_RS03350 - 673572..674486 (+) 915 WP_159543236.1 ABC transporter permease -
  K5J94_RS03355 - 674496..675527 (+) 1032 WP_159543234.1 ABC transporter permease -
  K5J94_RS03360 oppD 675540..676586 (+) 1047 WP_159564990.1 ABC transporter ATP-binding protein Regulator
  K5J94_RS03365 amiF 676586..677518 (+) 933 WP_159565000.1 ABC transporter ATP-binding protein Regulator
  K5J94_RS03370 - 677760..677997 (+) 238 Protein_613 Blp family class II bacteriocin -
  K5J94_RS03375 - 678052..678258 (+) 207 WP_221913522.1 bacteriocin -

Sequence


Protein


Download         Length: 310 a.a.        Molecular weight: 35046.17 Da        Isoelectric Point: 7.0889

>NTDB_id=597049 K5J94_RS03365 WP_159565000.1 676586..677518(+) (amiF) [Streptococcus halichoeri strain Shali_VAS-CPH]
MRDEGKKLVQLKNVSLTFNKGKKNEVKAIDNVSFDIYEGEVFGLVGESGSGKTTVGRAILKLYDISEGEIIFNGESISHL
KGKALHDFRKNAQMIFQDPQASLNGRMKIRDIVAEGLDIHKLTKNKAEREARVQELLDLVGLNKDHVTRYPHEFSGGQRQ
RIGIARALAVKPRFIIADEPISALDVSIQAQVVNLMQKLQHERGLTYLFIAHDLSMVKYISDRIGVMHWGKMLEIGTAED
VYNHPIHPYTKSLLSAIPEPDPAKERLRTADIYDPSQELDGQEREMREITPGHFVLSTEEEAMTYKTRIL

Nucleotide


Download         Length: 933 bp        

>NTDB_id=597049 K5J94_RS03365 WP_159565000.1 676586..677518(+) (amiF) [Streptococcus halichoeri strain Shali_VAS-CPH]
ATGCGTGATGAAGGCAAGAAATTAGTCCAACTAAAGAATGTGTCGCTAACCTTTAATAAGGGCAAGAAAAACGAAGTCAA
AGCTATTGATAATGTTAGTTTTGATATCTATGAAGGGGAAGTCTTTGGCCTTGTGGGAGAGTCAGGCTCTGGTAAGACCA
CTGTGGGCCGTGCTATTTTGAAATTGTATGATATTAGTGAGGGGGAAATCATTTTTAATGGCGAGTCTATTTCTCACTTA
AAAGGCAAAGCCTTACATGATTTTCGAAAAAATGCCCAGATGATTTTTCAAGATCCGCAAGCCAGTTTAAATGGTCGGAT
GAAAATTCGCGATATTGTCGCTGAAGGTTTGGACATTCATAAATTAACTAAGAATAAGGCTGAGCGTGAAGCACGAGTCC
AAGAATTACTTGACTTGGTTGGTTTGAATAAGGACCATGTTACCCGTTATCCACATGAATTTTCCGGTGGTCAACGCCAA
CGGATTGGCATTGCTCGCGCCCTAGCGGTTAAACCAAGATTTATCATTGCGGACGAACCGATTTCAGCATTAGATGTATC
CATTCAGGCGCAGGTGGTTAACTTGATGCAAAAATTGCAGCATGAACGTGGCTTAACCTATCTCTTTATTGCGCATGACC
TTTCCATGGTCAAATACATTTCTGATCGCATTGGGGTCATGCATTGGGGGAAGATGCTGGAAATTGGCACCGCAGAAGAT
GTCTATAATCACCCGATTCATCCGTATACGAAGAGCTTACTCTCAGCGATCCCAGAGCCTGATCCAGCAAAAGAGCGGCT
GCGGACAGCAGACATTTACGACCCAAGCCAAGAGCTGGATGGCCAAGAACGCGAAATGCGTGAGATTACACCAGGGCACT
TTGTCTTATCTACCGAAGAAGAAGCCATGACTTATAAAACCCGTATTCTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiF Streptococcus thermophilus LMD-9

55.446

97.742

0.542

  amiF Streptococcus thermophilus LMG 18311

55.116

97.742

0.539

  amiF Streptococcus salivarius strain HSISS4

54.785

97.742

0.535

  oppD Streptococcus mutans UA159

39.437

91.613

0.361