Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   K5J94_RS02880 Genome accession   NZ_CP081502
Coordinates   575372..576733 (+) Length   453 a.a.
NCBI ID   WP_159548488.1    Uniprot ID   -
Organism   Streptococcus halichoeri strain Shali_VAS-CPH     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 563770..576733 575372..576733 within 0


Gene organization within MGE regions


Location: 563770..576733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5J94_RS02815 - 563770..564348 (-) 579 WP_221913469.1 GTP pyrophosphokinase -
  K5J94_RS02820 - 564350..565096 (-) 747 WP_221913471.1 helix-turn-helix transcriptional regulator -
  K5J94_RS02825 - 565266..565427 (+) 162 WP_221913472.1 hypothetical protein -
  K5J94_RS02830 - 565631..566158 (+) 528 WP_221913475.1 5-formyltetrahydrofolate cyclo-ligase -
  K5J94_RS02835 - 566155..566826 (+) 672 WP_159564432.1 rhomboid family intramembrane serine protease -
  K5J94_RS02840 galU 566960..567865 (-) 906 WP_159548476.1 UTP--glucose-1-phosphate uridylyltransferase GalU -
  K5J94_RS02845 - 567889..568905 (-) 1017 WP_221913477.1 NAD(P)H-dependent glycerol-3-phosphate dehydrogenase -
  K5J94_RS02850 - 569101..569550 (+) 450 WP_221913479.1 MarR family winged helix-turn-helix transcriptional regulator -
  K5J94_RS02855 - 569543..571255 (+) 1713 WP_221913481.1 ABC transporter ATP-binding protein -
  K5J94_RS02860 - 571255..573039 (+) 1785 WP_221913483.1 ABC transporter ATP-binding protein -
  K5J94_RS02865 - 573312..574082 (+) 771 WP_159560508.1 epoxyqueuosine reductase QueH -
  K5J94_RS02870 - 574156..574533 (+) 378 WP_159548485.1 YbaN family protein -
  K5J94_RS02875 - 574850..575296 (+) 447 WP_221913485.1 dUTP diphosphatase -
  K5J94_RS02880 radA 575372..576733 (+) 1362 WP_159548488.1 DNA repair protein RadA Machinery gene

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49413.84 Da        Isoelectric Point: 7.3878

>NTDB_id=597046 K5J94_RS02880 WP_159548488.1 575372..576733(+) (radA) [Streptococcus halichoeri strain Shali_VAS-CPH]
MAKKKASYVCQECGYRSPKYLGRCPNCSAWSSFVEEVEVKEVKNARVSLSGEKSKPVKLKDVENIHYARTKTDMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLADKGTVLYVSGEESAEQIKLRSDRLGNRDNAFYLYAETNMQEIRAEIA
TIQPDFLIVDSIQTMMSPELTGVQGSVSQVREVTAELMQLAKTNQIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLADATGSAVVVTLEGSRPILAEVQALVTPTVFGNAR
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKAAGGVKLDEPAIDLAVAVAIASSYKDRPTDPQTAFLGEIGLTGELR
RVTRIEQRIKEAAKLGFTQIYVPKTALHGLDLPTGIQVHGAATVGQVLKAIFK

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=597046 K5J94_RS02880 WP_159548488.1 575372..576733(+) (radA) [Streptococcus halichoeri strain Shali_VAS-CPH]
TTGGCGAAAAAGAAAGCAAGTTATGTTTGTCAGGAATGTGGTTACCGTTCCCCTAAATATTTGGGGCGTTGCCCCAATTG
TTCCGCTTGGTCTTCTTTTGTAGAAGAGGTCGAGGTTAAGGAAGTCAAAAATGCCCGAGTTAGCCTAAGTGGGGAAAAAA
GCAAGCCCGTTAAGCTCAAGGATGTCGAAAATATCCATTATGCCAGGACCAAGACCGACATGGAGGAATTTAACCGGGTC
TTAGGTGGCGGTGTGGTACCAGGCAGCTTAGTCTTGATTGGAGGCGATCCAGGTATTGGCAAGTCAACCTTGCTGCTGCA
AGTGTCCACTCAGCTAGCAGATAAGGGGACCGTTCTGTATGTTTCAGGCGAAGAATCAGCAGAGCAGATCAAACTACGGA
GCGACCGTTTGGGCAATCGCGACAATGCTTTTTACCTCTATGCAGAAACCAATATGCAAGAGATTCGAGCAGAGATAGCC
ACGATCCAACCTGATTTTTTAATCGTTGACTCTATCCAAACCATGATGAGTCCAGAACTAACCGGTGTGCAAGGCTCAGT
CAGTCAAGTCCGAGAAGTGACTGCAGAGTTAATGCAGCTGGCAAAAACAAACCAGATAGCGACCTTCATTGTAGGGCATG
TCACCAAGGAAGGGACCCTTGCTGGACCGCGGATGTTAGAGCATATGGTGGATACTGTGCTTTATTTTGAGGGCGAGCGC
CATCATACTTTTCGTATCTTGCGGGCTGTCAAGAACCGTTTTGGTTCGACCAATGAAATTGGAATTTTTGAGATGCAATC
AGGCGGCCTGGTTGAAGTGCTCAATCCCAGTCAGGTTTTTCTGGAGGAGCGCTTGGCTGATGCGACAGGCTCAGCCGTTG
TGGTCACGCTAGAAGGCAGTCGGCCTATTCTTGCAGAGGTGCAAGCCCTGGTCACCCCGACCGTATTTGGTAATGCGCGG
CGCACAACCACTGGCTTAGACTTCAACCGAGTTAGTCTGATTATGGCGGTGCTTGAAAAACGCTGTGGTCTCTTGCTACA
AAATCAAGACGCTTATTTGAAGGCGGCTGGTGGAGTTAAGCTAGATGAGCCAGCCATTGATCTAGCAGTAGCGGTAGCGA
TTGCCTCTAGCTATAAGGACCGTCCTACCGACCCGCAGACTGCCTTTTTGGGTGAGATTGGCTTGACTGGGGAGTTGCGG
CGGGTGACGCGGATTGAGCAGCGGATCAAAGAGGCTGCTAAACTTGGTTTTACACAGATTTATGTGCCAAAAACTGCCCT
GCATGGTCTTGATTTGCCTACTGGGATTCAGGTGCATGGCGCAGCCACCGTGGGCCAAGTCCTAAAGGCTATCTTCAAGT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(66-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

83.407

99.779

0.832
  radA Streptococcus pneumoniae D39

83.407

99.779

0.832
  radA Streptococcus pneumoniae R6

83.407

99.779

0.832
  radA Streptococcus pneumoniae TIGR4

83.407

99.779

0.832
  radA Streptococcus mitis SK321

83.186

99.779

0.83
  radA Streptococcus mitis NCTC 12261

82.965

99.779

0.828
  radA Bacillus subtilis subsp. subtilis str. 168

62.252

100

0.623