Detailed information    

insolico Bioinformatically predicted

Overview

Name   recP/tkt   Type   Machinery gene
Locus tag   NAG71_RS06245 Genome accession   NZ_CP097883
Coordinates   1303200..1305203 (-) Length   667 a.a.
NCBI ID   WP_000087280.1    Uniprot ID   P33570
Organism   Escherichia coli str. K-12 substr. MG1655 strain K-12     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1298200..1310203
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NAG71_RS06230 aegA 1299314..1301293 (+) 1980 WP_001078880.1 formate-dependent uric acid utilization protein AegA -
  NAG71_RS06235 nudK 1301361..1301936 (+) 576 WP_001300814.1 GDP-mannose pyrophosphatase NudK -
  NAG71_RS06240 ypfG 1302062..1303105 (+) 1044 WP_001270542.1 DUF1176 domain-containing protein -
  NAG71_RS06245 recP/tkt 1303200..1305203 (-) 2004 WP_000087280.1 transketolase Machinery gene
  NAG71_RS06250 tal 1305223..1306173 (-) 951 WP_001003709.1 transaldolase -
  NAG71_RS06255 maeB 1306462..1308741 (+) 2280 WP_000342644.1 NADP-dependent oxaloacetate-decarboxylating malate dehydrogenase -
  NAG71_RS06260 eutS 1309034..1309369 (+) 336 WP_000356956.1 ethanolamine utilization microcompartment protein EutS -
  NAG71_RS06265 eutP 1309382..1309861 (+) 480 WP_000820763.1 ethanolamine utilization acetate kinase EutP -

Sequence

Protein


Download         Length: 667 a.a.        Molecular weight: 73042.72 Da        Isoelectric Point: 6.2536

>NTDB_id=597022 NAG71_RS06245 WP_000087280.1 1303200..1305203(-) (recP/tkt) [Escherichia coli str. K-12 substr. MG1655 strain K-12]
MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFLKHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYD
LPLEELKNFRQLHSKTPGHPEIGYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGHDPQAVKEAILEAQSVKDKPSLI
ICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKLGWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQL
AEEFTRRMSGGLPKDWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGSVSLKEDPA
GNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALMKARQIMVYTHDSIGLGEDGPTHQAVEQLAS
LRLTPNFSTWRPCDQVEAAVGWKLAVERHNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEM
EITLQAAEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGLKGAIVGMTGYGESAPA
DKLFPFFGFTAENIVAKAHKVLGVKGA

Nucleotide


Download         Length: 2004 bp        

>NTDB_id=597022 NAG71_RS06245 WP_000087280.1 1303200..1305203(-) (recP/tkt) [Escherichia coli str. K-12 substr. MG1655 strain K-12]
ATGTCCCGAAAAGACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGG
CGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGT
ATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGAC
CTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGG
CGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGG
CGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGT
ATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTC
CATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGCAAAACGTTTTGAAGCCTATCACTGGCATGTGATCCATG
AAATCGACGGTCACGATCCGCAGGCGGTGAAGGAAGCGATCCTTGAAGCGCAAAGCGTGAAAGATAAGCCGTCGCTGATT
ATCTGCCGTACGGTGATTGGCTTTGGTTCGCCGAATAAAGCAGGTAAGGAAGAGGCGCACGGCGCACCACTGGGGGAAGA
AGAAGTGGCGCTGGCACGGCAAAAACTGGGCTGGCACCATCCGCCATTTGAGATCCCTAAAGAGATTTATCACGCCTGGG
ATGCCCGTGAAAAAGGCGAAAAAGCGCAGCAGAGCTGGAATGAGAAGTTTGCCGCCTATAAAAAGGCTCATCCGCAACTG
GCAGAAGAGTTTACCCGACGGATGAGCGGTGGTTTACCGAAGGACTGGGAGAAAACGACTCAGAAATATATCAATGAGTT
ACAGGCAAATCCGGCGAAAATCGCTACCCGTAAGGCTTCGCAAAATACGCTTAACGCTTACGGGCCGATGCTGCCTGAGT
TGCTCGGCGGTTCGGCGGATCTGGCTCCCAGCAACCTGACCATCTGGAAAGGTTCTGTTTCGCTGAAGGAAGATCCAGCG
GGCAACTACATTCACTACGGGGTGCGTGAATTTGGCATGACCGCTATCGCCAACGGCATCGCGCACCACGGCGGCTTTGT
GCCGTATACCGCGACGTTCCTGATGTTTGTTGAATACGCCCGTAACGCCGCGCGGATGGCGGCACTGATGAAAGCGCGGC
AGATTATGGTTTATACCCACGACTCAATTGGCCTGGGCGAAGATGGTCCGACGCACCAGGCTGTTGAGCAACTGGCCAGC
CTGCGCTTAACGCCAAATTTCAGCACCTGGCGACCGTGCGATCAGGTGGAAGCGGCGGTGGGCTGGAAGCTGGCGGTTGA
GCGCCACAACGGACCGACGGCACTGATCCTCTCAAGGCAGAATCTGGCCCAGGTGGAACGTACGCCGGATCAGGTTAAAG
AGATTGCTCGTGGCGGTTATGTGCTGAAAGACAGCGGCGGTAAGCCAGATATTATTCTGATTGCCACCGGTTCAGAGATG
GAAATTACCCTGCAAGCGGCAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATAT
TTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTA
TTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTGTCGGGATGACGGGTTACGGGGAATCTGCTCCGGCG
GATAAGCTGTTCCCGTTCTTTGGCTTTACCGCCGAGAATATTGTGGCAAAAGCGCATAAGGTGCTGGGAGTGAAAGGTGC
CTGA

Domains

Predicted by InterProScan.

Transketolase, thiamine diphosphate binding domain (Transketolase_N)

(3-333)

Transketolase, pyrimidine binding domain (Transket_pyr)

(353-523)

Transketolase, C-terminal domain (Transketolase_C)

(539-654)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P33570

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recP/tkt Streptococcus pneumoniae TIGR4

47.496

98.801

0.469