Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NAG70_RS18645 Genome accession   NZ_CP097882
Coordinates   3798493..3798933 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli str. K-12 substr. MG1655 strain K-12     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3793493..3803933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NAG70_RS18625 aroP 3794491..3795864 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  NAG70_RS18630 ampE 3795907..3796761 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  NAG70_RS18635 ampD 3796758..3797309 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NAG70_RS18640 nadC 3797397..3798290 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NAG70_RS18645 pilA 3798493..3798933 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  NAG70_RS18650 pilB 3798943..3800328 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  NAG70_RS18655 hofC 3800318..3801520 (+) 1203 WP_000157266.1 protein transport protein HofC -
  NAG70_RS18660 guaC 3801555..3802598 (-) 1044 WP_001217338.1 GMP reductase -
  NAG70_RS18665 - 3802754..3802798 (-) 45 WP_120795372.1 protein YacM -
  NAG70_RS18670 coaE 3802823..3803443 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=596972 NAG70_RS18645 WP_000360904.1 3798493..3798933(+) (pilA) [Escherichia coli str. K-12 substr. MG1655 strain K-12]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=596972 NAG70_RS18645 WP_000360904.1 3798493..3798933(+) (pilA) [Escherichia coli str. K-12 substr. MG1655 strain K-12]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363