Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   NAG70_RS00340 Genome accession   NZ_CP097882
Coordinates   71171..71839 (+) Length   222 a.a.
NCBI ID   WP_001350561.1    Uniprot ID   B1X972
Organism   Escherichia coli str. K-12 substr. MG1655 strain K-12     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 66171..76839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NAG70_RS00310 yicC 66191..67054 (-) 864 WP_000621340.1 YicC/YloC family endoribonuclease -
  NAG70_RS00315 rph 67181..67896 (+) 716 Protein_61 ribonuclease PH -
  NAG70_RS00320 pyrE 67962..68603 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  NAG70_RS00325 slmA 68640..69236 (-) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  NAG70_RS00330 dut 69343..69801 (-) 459 WP_000976073.1 dUTP diphosphatase -
  NAG70_RS00335 coaBC 69779..70999 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  NAG70_RS00340 radC 71171..71839 (+) 669 WP_001350561.1 RadC family protein Machinery gene
  NAG70_RS00345 rpmB 72056..72292 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  NAG70_RS00350 rpmG 72313..72480 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  NAG70_RS00355 mutM 72578..73387 (+) 810 WP_001114543.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  NAG70_RS00360 coaD 73426..73905 (-) 480 WP_001171866.1 pantetheine-phosphate adenylyltransferase -
  NAG70_RS00365 waaA 73913..75190 (-) 1278 WP_000891564.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  NAG70_RS00370 rfaQ 75596..76666 (+) 1071 WP_001350560.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25343.46 Da        Isoelectric Point: 8.4958

>NTDB_id=596915 NAG70_RS00340 WP_001350561.1 71171..71839(+) (radC) [Escherichia coli str. K-12 substr. MG1655 strain K-12]
MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHRRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=596915 NAG70_RS00340 WP_001350561.1 71171..71839(+) (radC) [Escherichia coli str. K-12 substr. MG1655 strain K-12]
GTGAAAAACAATTCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACCGGG
TTATAACGCATAGGCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

UPF0758 N-terminal (UPF0758_N)

(9-77)

RadC-like JAB domain (RadC)

(103-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B1X972

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496