Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   K5A08_RS05575 Genome accession   NZ_CP081480
Coordinates   1084842..1085801 (+) Length   319 a.a.
NCBI ID   WP_002875425.1    Uniprot ID   -
Organism   Campylobacter jejuni strain NCTC12658     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1065138..1089844 1084842..1085801 within 0


Gene organization within MGE regions


Location: 1065138..1089844
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5A08_RS05480 (K5A08_05480) pglI 1065138..1066067 (-) 930 WP_002890470.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  K5A08_RS05485 (K5A08_05485) pglH 1066060..1067139 (-) 1080 WP_002859237.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  K5A08_RS05490 (K5A08_05490) pglK 1067136..1068830 (-) 1695 WP_002890469.1 ABC-type lipopolysaccharide transporter PglK -
  K5A08_RS05495 (K5A08_05495) galE 1068824..1069810 (-) 987 WP_002853529.1 UDP-glucose 4-epimerase GalE -
  K5A08_RS05500 (K5A08_05500) - 1069868..1070662 (-) 795 WP_002853812.1 3'-5' exonuclease -
  K5A08_RS05505 (K5A08_05505) waaC 1070728..1071756 (+) 1029 WP_002855693.1 lipopolysaccharide heptosyltransferase I -
  K5A08_RS05510 (K5A08_05510) htrB 1071746..1072633 (+) 888 WP_002890465.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  K5A08_RS05515 (K5A08_05515) - 1072630..1074177 (+) 1548 WP_002858148.1 glycosyltransferase family 2 protein -
  K5A08_RS05520 (K5A08_05520) - 1074181..1075353 (+) 1173 WP_221808001.1 glycosyltransferase family 2 protein -
  K5A08_RS05525 (K5A08_05525) - 1075337..1076332 (-) 996 WP_002858358.1 capsular polysaccharide synthesis protein -
  K5A08_RS05530 (K5A08_05530) - 1076390..1077559 (+) 1170 WP_002858320.1 glycosyltransferase family 2 protein -
  K5A08_RS05535 (K5A08_05535) wlaN 1077548..1078458 (-) 911 Protein_1080 beta-1,3 galactosyltransferase -
  K5A08_RS05540 (K5A08_05540) cstIII 1078513..1079397 (+) 885 WP_002858290.1 alpha-2,3-sialyltransferase -
  K5A08_RS05545 (K5A08_05545) neuB1 1079378..1080409 (+) 1032 WP_002858213.1 sialic acid synthase -
  K5A08_RS05550 (K5A08_05550) neuC 1080406..1081521 (+) 1116 WP_002858400.1 UDP-N-acetylglucosamine 2-epimerase -
  K5A08_RS08570 (K5A08_05555) - 1081518..1082456 (+) 939 Protein_1084 beta-1,4-N-acetylgalactosaminyltransferase -
  K5A08_RS08575 (K5A08_05560) - 1082466..1083128 (+) 663 WP_002881429.1 cytidylyltransferase domain-containing protein -
  K5A08_RS05565 (K5A08_05565) - 1083123..1083975 (-) 853 Protein_1086 glycosyltransferase -
  K5A08_RS05570 (K5A08_05570) waaV 1083959..1084783 (-) 825 WP_002858096.1 glycosyltransferase family 2 protein -
  K5A08_RS05575 (K5A08_05575) waaF 1084842..1085801 (+) 960 WP_002875425.1 lipopolysaccharide heptosyltransferase II Regulator
  K5A08_RS05580 (K5A08_05580) gmhA 1085782..1086342 (-) 561 WP_002858021.1 D-sedoheptulose 7-phosphate isomerase -
  K5A08_RS05585 (K5A08_05585) rfaE1 1086339..1087724 (-) 1386 WP_002890180.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  K5A08_RS05590 (K5A08_05590) rfaD 1087717..1088670 (-) 954 WP_002852832.1 ADP-glyceromanno-heptose 6-epimerase -
  K5A08_RS05595 (K5A08_05595) gmhB 1088671..1089231 (-) 561 WP_002878815.1 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  K5A08_RS05600 (K5A08_05600) - 1089313..1089615 (+) 303 WP_002852762.1 c-type cytochrome -
  K5A08_RS05605 (K5A08_05605) ccoS 1089638..1089844 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -

Sequence

Protein


Download         Length: 319 a.a.        Molecular weight: 36734.30 Da        Isoelectric Point: 10.2412

>NTDB_id=596769 K5A08_RS05575 WP_002875425.1 1084842..1085801(+) (waaF) [Campylobacter jejuni strain NCTC12658]
MKIFIHLPTWLGDTVMASPALYTIKEHFKDAQFILYGSFVSTALFKEFPNSKIIIENKLSRYKQALSLRKELGKIDLSFA
FRSAFSSKIILHILKTKQRYFFDKNKYKEEHQVLKYLYFIENSLSIKAHFKDLKLPFKLKFQNPLILRNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHDILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHLSAVYKVKTVAIFGPTKFTQTSPWQNENAKLVHLDLACMPCMQKTCPLKHHKCMKDLKPEKVIEQAKNLLKNSHL

Nucleotide


Download         Length: 960 bp        

>NTDB_id=596769 K5A08_RS05575 WP_002875425.1 1084842..1085801(+) (waaF) [Campylobacter jejuni strain NCTC12658]
ATGAAAATTTTTATACATCTTCCCACCTGGTTAGGCGATACGGTAATGGCTTCACCTGCTTTATACACTATAAAAGAACA
TTTTAAAGATGCTCAGTTTATCCTTTATGGTTCTTTTGTTTCTACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACTATCCCGTTATAAACAAGCTCTATCTTTACGCAAAGAACTTGGTAAAATCGATCTTAGCTTTGCT
TTTAGATCTGCGTTCTCTTCTAAGATTATCTTGCATATCCTTAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGTA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAATTCACTTAGTATAAAAGCTCATTTTAAGGACTTAA
AGCTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTATCTTAAGAAATGGTAAAAAAATTCTAGGACTCAACCCTGGTGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGATATTTT
AATCTTTGGTGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACCACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGACCTATGCACCTAAGTGCGGTTTATAAGGTAAAAACCGTAGCTATTTTTGGCCCTACGAAATTTACTCAAACTTCACC
TTGGCAAAATGAAAATGCAAAATTAGTGCATTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATCTAAAGCCTGAAAAAGTGATAGAACAGGCTAAAAATTTACTTAAAAACTCTCATCTATGA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(94-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

100

100

1