Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   K5K99_RS04530 Genome accession   NZ_CP081461
Coordinates   868204..869589 (+) Length   461 a.a.
NCBI ID   WP_114288852.1    Uniprot ID   A0A369B1J0
Organism   Vagococcus fluvialis strain 36B2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 863204..874589
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5K99_RS04515 (K5K99_04500) serC 864157..865227 (-) 1071 WP_167799225.1 3-phosphoserine/phosphohydroxythreonine transaminase -
  K5K99_RS04520 (K5K99_04505) - 865457..867475 (+) 2019 WP_114288962.1 KUP/HAK/KT family potassium transporter -
  K5K99_RS04525 (K5K99_04510) - 867581..868105 (+) 525 WP_114288851.1 dUTP diphosphatase -
  K5K99_RS04530 (K5K99_04515) radA 868204..869589 (+) 1386 WP_114288852.1 DNA repair protein RadA Machinery gene
  K5K99_RS04535 (K5K99_04520) - 869593..870690 (+) 1098 WP_114288853.1 PIN/TRAM domain-containing protein -
  K5K99_RS04540 (K5K99_04525) gltX 870798..872261 (+) 1464 WP_114288854.1 glutamate--tRNA ligase -
  K5K99_RS04545 (K5K99_04530) epsC 872519..873031 (+) 513 WP_086342017.1 serine O-acetyltransferase EpsC -
  K5K99_RS04550 (K5K99_04535) cysS 873040..874449 (+) 1410 WP_207059125.1 cysteine--tRNA ligase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 51117.54 Da        Isoelectric Point: 7.4011

>NTDB_id=596588 K5K99_RS04530 WP_114288852.1 868204..869589(+) (radA) [Vagococcus fluvialis strain 36B2]
MAKKKKNEFVCQECGYISPKYLGRCPNCGNWNTLVEEIIQEQATRHSRVSLTGESTKPQKLNQVTFKTEKRVQTDLEELN
RVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSQQLSKIDDNVLYVSGEESAEQIKMRAERLDSENNNFYVYAETDMEIIKQ
TIEQLRPNYVVIDSIQTMIHPEIKSAAGSVSQVRETTAELMQIAKTNQITIFIVGHVTKEGSLAGPRMLEHMVDTVLYFE
GERHHTFRILRAVKNRFGSTNEIGIFEMRQEGLVEVKNPSEAFLEERLADATGSAIVCSMEGTRPILAEVQALITPSVFG
NANRTAAGLDRHRVSLIMAVLEKRSGLLLQNQDAYLKAAGGVKLDEPAIDLAIAVSIASSYKDQGTSPHECFIGEIGLTG
EVRRVNRIDQRVQEAKKLGFKKIYVPKNNLQGWKVPTGIDIIGVSTVSETLRKVFNQEGRK

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=596588 K5K99_RS04530 WP_114288852.1 868204..869589(+) (radA) [Vagococcus fluvialis strain 36B2]
ATGGCAAAGAAAAAGAAAAATGAGTTTGTTTGTCAAGAATGTGGCTATATTTCACCTAAGTATTTAGGGAGATGTCCTAA
TTGTGGTAATTGGAATACTCTCGTTGAAGAAATTATACAAGAGCAAGCAACTCGCCATAGTAGAGTGAGCTTAACAGGTG
AATCAACAAAACCACAAAAATTAAATCAAGTAACCTTCAAAACTGAAAAAAGGGTGCAAACAGATTTAGAAGAATTAAAT
AGAGTTCTAGGTGGTGGCGTGGTTCCTGGCTCTATGGTGTTAATTGGGGGAGACCCTGGTATTGGTAAGTCAACCTTACT
CTTACAAGTCTCACAACAATTAAGTAAAATTGATGACAATGTGCTTTATGTTTCAGGTGAGGAAAGTGCTGAGCAGATAA
AAATGCGCGCTGAACGATTAGATTCTGAAAATAATAATTTTTATGTTTATGCTGAAACAGATATGGAAATTATTAAACAA
ACTATTGAGCAACTGCGTCCTAACTATGTAGTTATTGATTCGATTCAAACCATGATACACCCAGAAATTAAAAGTGCCGC
AGGAAGTGTTAGCCAAGTAAGAGAAACAACAGCTGAGTTGATGCAGATTGCTAAGACTAACCAAATTACGATTTTTATTG
TTGGGCATGTAACAAAAGAAGGCTCTCTTGCTGGACCAAGAATGTTAGAGCATATGGTAGATACAGTCCTTTATTTTGAA
GGAGAGCGTCATCATACTTTCAGAATTTTAAGAGCTGTAAAAAATCGGTTTGGTTCAACGAATGAAATTGGTATTTTTGA
GATGCGACAAGAAGGACTGGTTGAAGTGAAAAATCCTTCTGAAGCCTTTTTAGAAGAGCGACTAGCAGATGCCACAGGTT
CTGCTATTGTCTGTTCAATGGAAGGAACGCGTCCGATATTAGCTGAGGTACAAGCTTTAATTACACCATCAGTTTTTGGA
AATGCTAATAGAACCGCAGCGGGGCTTGATCGTCACCGAGTTTCACTGATTATGGCTGTTTTAGAAAAAAGATCTGGTCT
TTTACTACAAAATCAAGATGCTTATCTGAAAGCAGCAGGTGGTGTGAAGTTAGATGAGCCAGCGATTGACTTAGCGATTG
CTGTTAGTATTGCTTCAAGCTACAAAGATCAAGGGACCTCACCTCATGAGTGTTTCATTGGAGAGATTGGTTTGACTGGT
GAAGTTAGACGAGTTAATCGTATTGATCAACGTGTTCAAGAGGCTAAAAAATTAGGTTTTAAAAAAATCTATGTACCAAA
AAATAATTTACAAGGTTGGAAAGTACCAACAGGAATTGATATAATCGGAGTTTCCACAGTTTCAGAGACACTGAGAAAAG
TCTTTAATCAAGAAGGGAGGAAATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

AAA domain (AAA_25)

(69-221)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A369B1J0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

72.308

98.698

0.714
  radA Streptococcus pneumoniae D39

72.308

98.698

0.714
  radA Streptococcus pneumoniae R6

72.308

98.698

0.714
  radA Streptococcus pneumoniae TIGR4

72.308

98.698

0.714
  radA Streptococcus mitis SK321

72.308

98.698

0.714
  radA Streptococcus mitis NCTC 12261

72.308

98.698

0.714
  radA Bacillus subtilis subsp. subtilis str. 168

66.741

97.831

0.653