Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   K4756_RS12725 Genome accession   NZ_CP081458
Coordinates   2471975..2472385 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis subsp. subtilis strain ps4060     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2466975..2477385
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K4756_RS12695 (K4756_12625) - 2467062..2467202 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  K4756_RS12700 (K4756_12630) yqeF 2467508..2468239 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  K4756_RS12705 (K4756_12635) cwlH 2468491..2469243 (-) 753 WP_015483449.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  K4756_RS12710 (K4756_12640) yqeD 2469430..2470056 (+) 627 WP_015384137.1 TVP38/TMEM64 family protein -
  K4756_RS12715 (K4756_12645) gnd 2470075..2470968 (-) 894 WP_015384138.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  K4756_RS12720 (K4756_12650) yqeB 2471220..2471942 (+) 723 WP_015384139.1 hypothetical protein -
  K4756_RS12725 (K4756_12655) nucA/comI 2471975..2472385 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  K4756_RS12730 (K4756_12660) sigK 2472581..2473309 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  K4756_RS12735 (K4756_12665) - 2473391..2473582 (-) 192 Protein_2463 recombinase family protein -
  K4756_RS12740 (K4756_12670) - 2473540..2473704 (-) 165 WP_015483452.1 hypothetical protein -
  K4756_RS12745 (K4756_12675) psiE 2474244..2474660 (-) 417 WP_003246154.1 phosphate-starvation-inducible protein PsiE -
  K4756_RS12750 (K4756_12680) yrkQ 2474706..2476004 (-) 1299 WP_015483453.1 two-component system sensor histidine kinase YrkQ -
  K4756_RS12755 (K4756_12685) yrkP 2475991..2476686 (-) 696 WP_015384143.1 two-component response regulator YrkP -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=596516 K4756_RS12725 WP_009967785.1 2471975..2472385(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain ps4060]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=596516 K4756_RS12725 WP_009967785.1 2471975..2472385(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain ps4060]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGTGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529