Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AW27_RS08985 Genome accession   NZ_CP097800
Coordinates   2225118..2226332 (-) Length   404 a.a.
NCBI ID   WP_037928316.1    Uniprot ID   -
Organism   Streptomyces sp. PCS3-D2 isolate P1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2220118..2231332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AW27_RS08965 (AW27_008945) - 2220397..2220735 (-) 339 WP_037928327.1 P-II family nitrogen regulator -
  AW27_RS08970 (AW27_008950) - 2220732..2222075 (-) 1344 WP_037928324.1 ammonium transporter -
  AW27_RS08975 (AW27_008955) - 2222438..2223928 (-) 1491 WP_037928321.1 hypothetical protein -
  AW27_RS08980 (AW27_008960) - 2224376..2225044 (+) 669 WP_037928724.1 bifunctional DNA primase/polymerase -
  AW27_RS08985 (AW27_008965) pilA 2225118..2226332 (-) 1215 WP_037928316.1 signal recognition particle-docking protein FtsY Machinery gene
  AW27_RS08990 (AW27_008970) - 2226642..2227628 (+) 987 WP_037928313.1 LLM class flavin-dependent oxidoreductase -
  AW27_RS08995 (AW27_008975) - 2227670..2229103 (-) 1434 WP_037928308.1 sugar porter family MFS transporter -

Sequence

Protein


Download         Length: 404 a.a.        Molecular weight: 42209.32 Da        Isoelectric Point: 4.4268

>NTDB_id=596499 AW27_RS08985 WP_037928316.1 2225118..2226332(-) (pilA) [Streptomyces sp. PCS3-D2 isolate P1]
MDILILAVVIALVAVGVISGLVVSSRKKKQLPPPAPQSTPTITAPPAEPQVGEDAVETAEEPRRTIEEVGLPGAGAPVEA
PAAEPEAVPAAPEIEVPEPAAGRLVRLRARLARSQNSLGKGLLTLLSREHLDEDTWEEIEETLLVADVGVVPTQELVERL
RERVKVLGTRTPADLRALLKEELLTLVGTDFDRAVKTESGEDTPGVIMVVGVNGTGKTTTTGKLARVLVADGRSVVLGAA
DTFRAAAADQLQTWGERVGARTVRGPEGGDPASIAYDAVKEGIAEGADVVLIDTAGRLHTKTGLMDELGKVKRVVEKHGP
LDEILLVLDATTGQNGLTQARVFAEVVDITGIVLTKLDGTAKGGIVVAVQRELGVPVKLVGLGEGADDLAPFEPEAFVDA
LIGD

Nucleotide


Download         Length: 1215 bp        

>NTDB_id=596499 AW27_RS08985 WP_037928316.1 2225118..2226332(-) (pilA) [Streptomyces sp. PCS3-D2 isolate P1]
ATGGACATCCTCATCCTTGCTGTAGTCATCGCCCTGGTCGCGGTCGGCGTGATCAGCGGGCTCGTGGTCAGCAGCCGCAA
GAAGAAGCAGCTGCCGCCCCCGGCACCGCAGAGCACGCCGACCATCACTGCCCCGCCCGCCGAGCCGCAGGTGGGGGAGG
ACGCCGTAGAGACGGCGGAAGAGCCGCGCCGCACGATCGAGGAGGTCGGGCTCCCGGGAGCCGGGGCTCCCGTCGAAGCG
CCGGCCGCCGAGCCGGAGGCGGTCCCGGCCGCGCCCGAGATCGAGGTGCCCGAGCCCGCCGCCGGCCGCCTGGTCCGGCT
CCGCGCGCGTCTCGCCCGTTCGCAGAACTCCCTCGGCAAGGGACTGCTCACGCTGCTCTCGCGCGAGCACCTCGACGAGG
ACACCTGGGAGGAGATCGAGGAGACCCTCCTCGTCGCCGACGTCGGCGTCGTGCCCACCCAGGAGCTCGTCGAGCGGCTC
CGTGAGCGGGTCAAGGTCCTCGGCACCCGCACTCCGGCCGATCTGCGCGCCCTGCTCAAGGAAGAGCTGCTGACCCTGGT
CGGCACCGACTTCGACCGGGCCGTGAAGACGGAGAGCGGCGAGGACACCCCCGGCGTGATCATGGTCGTCGGCGTCAACG
GCACCGGCAAGACCACCACCACCGGCAAGCTGGCCCGGGTGCTCGTCGCGGACGGCCGCAGCGTGGTGCTCGGCGCGGCC
GACACCTTCCGCGCCGCGGCCGCCGACCAGCTGCAGACCTGGGGCGAGCGCGTCGGCGCCCGTACCGTCCGCGGCCCCGA
GGGCGGCGACCCGGCCTCGATCGCCTACGACGCGGTCAAGGAGGGCATCGCCGAGGGTGCCGACGTCGTGCTCATCGACA
CCGCCGGCCGACTGCACACCAAGACCGGCCTCATGGACGAGCTCGGCAAGGTCAAGCGCGTCGTCGAGAAGCACGGCCCG
CTGGACGAGATCCTGCTGGTCCTGGACGCCACCACGGGGCAGAACGGCCTGACCCAGGCGCGTGTCTTCGCCGAGGTCGT
CGACATCACCGGCATCGTGCTCACCAAGCTCGACGGCACGGCCAAGGGCGGCATCGTCGTCGCCGTCCAGCGCGAACTGG
GCGTCCCGGTCAAGCTCGTCGGTCTCGGCGAGGGTGCGGACGACCTGGCTCCGTTCGAGCCCGAGGCGTTCGTGGACGCC
CTGATCGGCGACTGA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(118-183)

SRP54-type protein, GTPase domain (SRP54)

(205-402)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

42.391

91.089

0.386