Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   K4756_RS01930 Genome accession   NZ_CP081458
Coordinates   363307..363756 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis subsp. subtilis strain ps4060     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 358307..368756
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K4756_RS01895 (K4756_01895) yckA 358452..359132 (-) 681 WP_015382730.1 amino acid ABC transporter permease -
  K4756_RS01900 (K4756_01900) yckB 359142..360005 (-) 864 WP_015482776.1 transporter substrate-binding domain-containing protein -
  K4756_RS01905 (K4756_01905) - 360167..360364 (+) 198 WP_144052033.1 hypothetical protein -
  K4756_RS01910 (K4756_01910) yckC 360414..360845 (+) 432 WP_046340202.1 RDD family protein -
  K4756_RS01915 (K4756_01915) yckD 360927..361259 (+) 333 WP_015252849.1 YckD family protein -
  K4756_RS01920 (K4756_01920) bglC 361412..362845 (+) 1434 WP_015382735.1 6-phospho-beta-glucosidase -
  K4756_RS01925 (K4756_01925) nin/comJ 362882..363280 (-) 399 WP_015382736.1 DNA-entry nuclease inhibitor Regulator
  K4756_RS01930 (K4756_01930) nucA/comI 363307..363756 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  K4756_RS01935 (K4756_01935) tlpC 363922..365643 (-) 1722 WP_041517645.1 methyl-accepting chemotaxis protein TlpC -
  K4756_RS01940 (K4756_01940) hxlB 365754..366311 (-) 558 WP_015482781.1 6-phospho-3-hexuloisomerase -
  K4756_RS01945 (K4756_01945) hxlA 366317..366949 (-) 633 WP_015482782.1 3-hexulose-6-phosphate synthase -
  K4756_RS01950 (K4756_01950) hxlR 367181..367543 (+) 363 WP_015482783.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=596473 K4756_RS01930 WP_014475770.1 363307..363756(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain ps4060]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=596473 K4756_RS01930 WP_014475770.1 363307..363756(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain ps4060]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGTCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGATATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987