Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   M9O75_RS18455 Genome accession   NZ_CP097724
Coordinates   3813117..3813557 (+) Length   146 a.a.
NCBI ID   WP_000360913.1    Uniprot ID   -
Organism   Escherichia coli strain MS1494     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3808117..3818557
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9O75_RS18435 (M9O75_18435) aroP 3809117..3810487 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  M9O75_RS18440 (M9O75_18440) ampE 3810530..3811384 (-) 855 WP_000171984.1 beta-lactamase regulator AmpE -
  M9O75_RS18445 (M9O75_18445) ampD 3811381..3811932 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M9O75_RS18450 (M9O75_18450) nadC 3812020..3812913 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M9O75_RS18455 (M9O75_18455) pilA 3813117..3813557 (+) 441 WP_000360913.1 prepilin peptidase-dependent pilin Machinery gene
  M9O75_RS18460 (M9O75_18460) pilB 3813567..3814952 (+) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  M9O75_RS18465 (M9O75_18465) hofC 3814942..3816144 (+) 1203 WP_000157243.1 protein transport protein HofC -
  M9O75_RS18470 (M9O75_18470) guaC 3816179..3817222 (-) 1044 WP_001217338.1 GMP reductase -
  M9O75_RS18475 (M9O75_18475) - 3817378..3817422 (-) 45 WP_120795372.1 protein YacM -
  M9O75_RS18480 (M9O75_18480) coaE 3817447..3818067 (+) 621 WP_001269524.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15634.80 Da        Isoelectric Point: 4.5237

>NTDB_id=596372 M9O75_RS18455 WP_000360913.1 3813117..3813557(+) (pilA) [Escherichia coli strain MS1494]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSIVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=596372 M9O75_RS18455 WP_000360913.1 3813117..3813557(+) (pilA) [Escherichia coli strain MS1494]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCATCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363